In the early spring of 1999, I was conducting fieldwork on agricultural development in a township in northeast Yunnan.This study is a result of my fieldwork carried out in February–March 1999 and again in December 1999 in the township where I conducted household surveys in three villages (20–30 households in each, depending on the size of village, by random sampling) and interviews with village leaders and officials at township and county levels. The township, which I call Banyan, is under the jurisdiction of a county-city (xianji shi) that acquired its urban status in 1994. One afternoon, as I was sitting by a kitchen fire interviewing a housewife in a village two kilometres from the township seat, some people from the neighbourhood walked in, and it did not take long before a crowd gathered in the room. No sooner had I concluded my interview when voices roared from the audience. Taken by the villagers for a reporter, I was bombarded with bitter accounts of the ongoing land expropriation in their community.

The goal of this study is to quantitatively intercompare the standard products of the Tropical Rainfall Measuring Mission (TRMM) Multisatellite Precipitation Analysis (TMPA) and its successor, the Global Precipitation Measurement (GPM) mission Integrated Multisatellite Retrievals for GPM (IMERG), with a dense gauge network over the midlatitude Ganjiang River basin in southeast China. In general, direct comparisons of the TMPA 3B42V7, 3B42RT, and GPM Day-1 IMERG estimates with gauge observations over an extended period of the rainy season (from May through September 2014) at 0.25° and daily resolutions show that all three products demonstrate similarly acceptable (~0.63) and high (0.87) correlation at grid and basin scales, respectively, although 3B42RT shows much higher overestimation. Both of the post-real-time corrections effectively reduce the bias of Day-1 IMERG and 3B42V7 to single digits of underestimation from 20+% overestimation of 3B42RT. The Taylor diagram shows that Day-1 IMERG and 3B42V7 are comparable at grid and basin scales. Hydrologic assessment with the Coupled Routing and Excess Storage (CREST) hydrologic model indicates that the Day-1 IMERG product performs comparably to gauge reference data. In many cases, the IMERG product outperforms TMPA standard products, suggesting a promising prospect of hydrologic utility and a desirable hydrologic continuity from TRMM-era product heritages to GPM-era IMERG products. Overall, this early study highlights that the Day-1 IMERG product can adequately substitute TMPA products both statistically and hydrologically, even with its limited data availability to date, in this well-gauged midlatitude basin. As more IMERG data are released, more studies to explore the potential of GPM-era IMERG in water, weather, and climate research are urgently needed.

BackgroundLong noncoding RNAs (lncRNAs) are an important class of pervasive genes involved in a variety of biological functions. They are aberrantly expressed in many types of cancers. In this study, we described lncRNAs profiles in 6 pairs of human renal clear cell carcinoma (RCCC) and the corresponding adjacent nontumorous tissues (NT) by microarray.Methodology/Principal FindingsWith abundant and varied probes accounting 33,045 LncRNAs in our microarray, the number of lncRNAs that expressed at a certain level could be detected is 17157. From the data we found there were thousands of lncRNAs that differentially expressed (≥2 fold-change) in RCCC tissues compared with NT and 916 lncRNAs differentially expressed in five or more of six RCCC samples. Compared with NT, many lncRNAs were significantly up-regulated or down-regulated in RCCC. Our data showed that down-regulated lncRNAs were more common than up-regulated ones. ENST00000456816, X91348, BC029135, NR_024418 were evaluated by qPCR in sixty-three pairs of RCCC and NT samples. The four lncRNAs were aberrantly expressed in RCCC compared with matched histologically normal renal tissues.Conclusions/SignificanceOur study is the first one to determine genome-wide lncRNAs expression patterns in RCCC by microarray. The results displayed that clusters of lncRNAs were aberrantly expressed in RCCC compared with NT samples, which revealed that lncRNAs differentially expressed in tumor tissues and normal tissues may exert a partial or key role in tumor development. Taken together, this study may provide potential targets for future treatment of RCCC and novel insights into cancer biology.

BackgroundThere is increasing evidence to suggest that miRNAs play an important role in predicting cancer survival. To identify a panel of miRNA signature that can divided tumor from normal bladder using miRNA expression levels, and to assess the prognostic value of this specific miRNA markers in bladder cancer (BCa).MethodsA comprehensive meta-review of published miRNA expression profiles that compared BCa and adjacent normal tissues was performed to determine candidate miRNAs as prognostic biomarkers for BCa. Vote-counting strategy and Robust Rank Aggregation method were used to identify significant meta-signature miRNAs.ResultsWe identified an eight-miRNA signature including three upregulated (miR-141, miR-200c, miR-21) and five downregulated (miR-145, miR-125, miR-199a, let-7c and miR-99a) miRNAs for the prediction of overall survival (OS) using TCGA dataset, and validated in our 48 BCa patients. X-tile plot was used to generate the optimum cut-off point and Kaplan-Meier method was used to calculate OS. A linear prognostic model of eight miRNAs was constructed and weighted by the importance scores from the supervised principal component method to divide patients into high- and low-risk groups. Patients assigned to the high-risk group were associated with poor OS compared with patients in the low-risk group (HR = 5.21, p < 0.001). Our validation cohort of 48 patients confirmed the panel of 8-miRNAs as a reliable prognostic tool for OS in patients with BCa (HR = 5.04, p < 0.001).ConclusionThe present meta-analysis identified eight highly significant and consistently dysregulated miRNAs from 19 datasets. We also constructed an eight-miRNA signature which provided predictive and prognostic value that complements traditional clinicopathological risk factors.Electronic supplementary materialThe online version of this article (doi:10.1186/s13046-015-0167-0) contains supplementary material, which is available to authorized users.

Background InformationDysregulated micro‐RNAs have been reported in many human cancers, including renal cell carcinoma. Recent studies indicated that miR‐490 is involved in tumour development and progression. However, the expression profile and function in renal cell carcinoma remains unknown.ResultsHerein, we showed that miR‐490‐5p was down‐regulated in renal cell carcinoma tissues and cells compared with the adjacent normal tissues and normal cells. We also provided evidence that miR‐490‐5p acts as a tumour suppressor in renal carcinoma in a variety of in vitro and in vivo assays. Mechanistically, miR‐490‐5p was verified to directly bind to 3′ UTR of the PIK3CA mRNA and reduce the expression of PIK3CA at both mRNA and protein levels, which further inhibits phosphatidylinositol 3‐kinase/Akt signalling pathway. We further showed that knockdown of PIK3CA can block the growth inhibitory effect of miR‐490‐5p, and over‐expression of PIK3CA can reverse the inhibitory effect of miR‐490‐5p on renal cancer cell tumourigenicity.ConclusionsTaken together, our results indicated for the first time that miR‐490‐5p functions as a tumour suppressor in renal carcinoma by targeting PIK3CA.SignificanceOur findings suggest that miR‐490‐5p may be a potential gene therapy target for the treatment of renal cell carcinoma.

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Retroperitoneal laparoscopic dismembered pyeloplasty is a minimally invasive, safe and effective therapy for ureteropelvic junction obstruction with low morbidity, shorter convalescence and excellent outcomes, and can be accomplished reasonably quickly in experienced hands.

In the early experience, our data suggest that RA, compared with LA, may be a safe and feasible option associated with less blood loss and shorter hospital stay when performed by experienced surgeons in selected patients.