Discovery of lunasin peptide in triticale (X Triticosecale Wittmack)

Nakurte, Ilva; Klavins, Kristaps; Kirhnere, Inga; Namniece, Jana; Adlere, Liene; Matvejevs, Jaroslavs; Kronberga, Arta; Kokare, Aina; Strazdiņa, Vija; Legzdiņa, Linda; Muceniece, Ruta

doi:10.1016/j.jcs.2012.04.004

Cited by 37 publications

(46 citation statements)

References 17 publications

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“…26,27 On the other hand, the application of MS to assess digests allowed, for the first time, to identify the peptides released from lunasin by the action of digestive enzymes. On one hand, this analysis allowed quantification of lunasin that remained intact after gastric and gastro-duodenal digestion.…”

Section: Discussionmentioning

confidence: 99%

The protective role of the Bowman-Birk protease inhibitor in soybean lunasin digestion: the effect of released peptides on colon cancer growth

et al. 2015

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Lunasin is a naturally-occurring peptide demonstrating chemopreventive, antioxidant and anti-inflammatory properties. To exhibit these activities, orally ingested lunasin needs to survive proteolytic attack of digestive enzymes to reach target tissues in active form/s. Preliminary studies suggested the protective role of protease inhibitors, such as the Bowman-Birk inhibitor and Kunitz-trypsin inhibitor, against lunasin's digestion by both pepsin and pancreatin. This work describes in depth the behaviour of lunasin under conditions simulating the transit through the gastrointestinal tract in the absence or presence of soybean Bowman-Birk isoinhibitor 1 (IBB1) in both active and inactive states. By liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS), the remaining lunasin at the end of gastric and gastro-duodenal phases was quantified. Protection against the action of pepsin was independent of the amount of IBB1 present in the analyzed samples, whereas an IBB1 dose-dependent protective effect against trypsin and chymotrypsin was observed. Peptides released from lunasin and inactive IBB1 were identified by MS/MS. The remaining lunasin and IBB1 as well as their derived peptides could be responsible for the anti-proliferative activity against colon cancer cells observed for the digests obtained at the end of simulated gastrointestinal digestion.

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Section: Discussionmentioning

confidence: 99%

The protective role of the Bowman-Birk protease inhibitor in soybean lunasin digestion: the effect of released peptides on colon cancer growth

et al. 2015

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“…In the last decade, the interest in bioactive peptides from natural products and food, such as cereals, has increased and in this area one of the most studied natural peptides was lunasin. Lunasin was identified by several research groups in different wheat products: kernels (Jeong et al., ; Nakuerte et al., ), whole wheat flour and dough (Rizzello, Nionelli, Coda, & Gobbetti, ). The presence of lunasin was also reported in rye (Jeong et al., ) triticale (Nakuerte et al., ) and barley (Jeong, Lam, & de Lumen, ).…”

Section: Introductionmentioning

confidence: 99%

“…Lunasin was identified by several research groups in different wheat products: kernels (Jeong et al., ; Nakuerte et al., ), whole wheat flour and dough (Rizzello, Nionelli, Coda, & Gobbetti, ). The presence of lunasin was also reported in rye (Jeong et al., ) triticale (Nakuerte et al., ) and barley (Jeong, Lam, & de Lumen, ). Several biological activities of lunasin extracted from wheat and other straw cereals, including cancer‐preventive properties and inhibition of core‐histone acetylation have been reported (Chang et al., ; Galvez & De Lumen, ; Jeong et al., ).…”

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of Wheat Derived Nonspecific Lipid Transfer Protein 2 (nsLTP2)

Bosi

Fiori

Dinelli

et al. 2018

Journal of Food Science

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Numerous studies support the protective role of bioactive peptides against cardiovascular diseases. Cereals represent the primary source of carbohydrates, but they also contain substantial amounts of proteins, therefore representing a potential dietary source of bioactive peptides with nutraceutical activities. The analysis of wheat extracts purified by chromatographic techniques by means of HPLC-UV/nanoLC-nanoESI-QTOF allowed the identification of a signal of about 7 kDa which, following data base searches, was ascribed to a nonspecific lipid-transfer protein (nsLTP) type 2 from Triticum aestivum (sequence coverage of 92%). For the first time nsLTP2 biological activities have been investigated. In particular, in experiments with human umbilical vein endothelial cells (HUVEC), nsLTP2 displayed antioxidant and cytoprotective activities, being able to significantly decrease reactive oxygen species (ROS) levels and to reduce lactate dehydrogenase (LDH) release, generated following oxidative (hydrogen peroxide) and inflammatory (tumor necrosis factor α, interleukin-1β, and lipopolysaccharide) stimulation. The obtained promising results suggest potential protective role of nsLTP2 in vascular diseases prevention. PRACTICAL APPLICATION: nsLTP 2 peptide is resistant to proteases throughout the gastrointestinal tract and exerts antioxidant and cytoprotective activities. These characteristics could be exploited in vascular diseases prevention.

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“…Its presence was detected in both cereals and non‐cereal plants with variable content. In cereals, this content ranged from 13 to 99 μg g −1 seed in barley, 200–300 μg g −1 seed in wheat, 45–150 μg g −1 seed in rye, 429–6458 μg g −1 grain in triticale, and 34–197 μg g −1 grain in oat . The content was reported to be mainly influenced by the genotype .…”

Section: Introductionmentioning

confidence: 99%

Updating the research on the chemopreventive and therapeutic role of the peptide lunasin

et al. 2017

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Chronic diseases have become the medical challenge of the 21st century because of their high incidence and mortality rates. Modulation of diet and lifestyle habits is considered as the best strategy for the prevention of these disorders. Health promoting benefits beyond their nutritional effects have been described for multiple dietary compounds. Among these compounds, the peptide lunasin is considered as one of the most promising. Naturally present in soybean, lunasin has been extensively studied in the last two decades because of its potential against chronic diseases such as cancer, cardiovascular and immunological disorders. The purpose of this article is to summarise the evidence on the presence of lunasin in soybean and derived foods, and its bioavailability once it is orally ingested. The protective and therapeutic effects of this peptide against cancer, oxidative stress, inflammation, and high cholesterol levels as well as the molecular mechanisms of action involved in these effects are also described in this review. © 2017 Society of Chemical Industry.

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Discovery of lunasin peptide in triticale (X Triticosecale Wittmack)

Cited by 37 publications

References 17 publications

The protective role of the Bowman-Birk protease inhibitor in soybean lunasin digestion: the effect of released peptides on colon cancer growth

The protective role of the Bowman-Birk protease inhibitor in soybean lunasin digestion: the effect of released peptides on colon cancer growth

Isolation and Characterization of Wheat Derived Nonspecific Lipid Transfer Protein 2 (nsLTP2)

Updating the research on the chemopreventive and therapeutic role of the peptide lunasin

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