Discovery of chemically induced mutations in rice by TILLING

Till, Bradley J.; Cooper, Jennifer L.; Tai, Thomas H.; Colowit, Peter M.; Greene, Elizabeth A.; Henikoff, Steven; Comai, Luca

doi:10.1186/1471-2229-7-19

Cited by 424 publications

(392 citation statements)

References 44 publications

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“…mainly GC to AT transition (Till et al 2007). Since the rice genome can tolerate a large number of point mutations, it is possible that each of the mutants carry multiple changes.…”

Section: Resultsmentioning

confidence: 99%

“…Therefore 0.6 to 1.0 % was considered the optimal level of EMS dosage for mutagen treatment and 0.8% was used for generation of mutant population in subsequent experiments. In contrast, more than 1.5% EMS has been found more effective in case of indica variety IR64 (Wu et al 2005;Till et al 2007). In Arabidopsis, EMS-induced mutants were generated in the genetic backgrounds of Columbia (Col) and Landsberg erecta (Ler).…”

Section: Resultsmentioning

confidence: 99%

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EMS Induced Mutants of Upland Rice Variety Nagina22: Generation and Characterization

Mohapatra¹,

Robin²,

Sarla³

et al. 2014

Proceedings of the Indian National Science Academy

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One of the approaches for understanding the functions of the genes predicted in the rice genome requires use of mutants. Mutations induced in a given genetic background provide opportunities to assign function to a given gene with a minimum of background genetic noise. This paper describes generation and initial characterization of a large set of Ethyl Methane Sulphonate (EMS) induced mutants in the upland rice variety Nagina22, through a National Initiative involving six Research Institutes namely National Research Centre on Plant Biotechnology, New Delhi; Indian Agricultural Research Institute, New Delhi; Tamil Nadu Agricultural University, Coimbatore; Directorate of Rice Research, Hyderabad; University of Agricultural Sciences, Bangalore and Punjab Agricultural University, Ludhiana, funded by the Department of Biotechnology (DBT), Government of India. The uniqueness of this collaborative effort is phenotyping for a range of traits that has led to identification of mutants for plant growth and architecture, flowering, maturity, grain number, shape and size, yield, phosphorus use efficiency, resistance to blast and bacterial leaf blight diseases, and tolerance to drought, salinity and herbicide. A set of 22, 292 mutagenised lines generated under this initiative and phenotyped for the traits enlisted above has resulted in the isolation of a few promising mutants which are being characterized. Shortly, these mutants will be registered and made available to the researchers in the country for use in studies in rice genetics, breeding and functional genomics. The mutant stock is expected to serve as a national resource for understanding rice biology as well as for use in genetic improvement of the crop.

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“…mainly GC to AT transition (Till et al 2007). Since the rice genome can tolerate a large number of point mutations, it is possible that each of the mutants carry multiple changes.…”

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

EMS Induced Mutants of Upland Rice Variety Nagina22: Generation and Characterization

Mohapatra¹,

Robin²,

Sarla³

et al. 2014

Proceedings of the Indian National Science Academy

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“…Advances in nutritional genomics [28 ] and efficient molecular marker techniques allow tracking complex traits along the breeding process. Powerful new techniques, such as TILLING -saturating metabolic pathways with mutations and subsequently identifying the genes involved [29] -add to the arsenal of molecular and genomic tools, such as the increasing number of fully sequenced genomes.…”

Section: Molecular Breeding and Transgenicsmentioning

confidence: 99%

Biofortified crops to alleviate micronutrient malnutrition

Mayer

Pfeiffer

Beyer

2008

Current Opinion in Plant Biology

426

268

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“…As in the case of the pi21 gene where good alleles exist in nature, one can expect to find interesting alleles of negative regulators by mutational approaches. With this respect, the Tilling technology (Till et al 2007) opens the possibility of finding agronomically interesting alleles of negative regulators.…”

Section: Conclusion and Recommendations For Future Transgenic Approamentioning

confidence: 99%

Potential Candidate Genes for Improving Rice Disease Resistance

Delteil

Zhang²,

Lessard³

et al. 2010

Rice

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Diseases caused by fungal and bacterial pathogens like Magnaporthe oryzae and Xanthomonas oryzae pv. oryzae are responsible for considerable yield loss. Up to now, in rice, the modification of the expression of more than 60 genes from diverse origins has shown beneficial effects with respect to disease resistance. In this paper, we review this large set of data to identify the best genes and strategies to achieve disease resistance by transgenic approaches. Altered expression of genes involved in signal transduction and transcription may lead to many unwanted side effects, like lesion mimic phenotypes. Moreover, modification of resistance to abiotic stress has been neglected and should be carefully examined in the future. Genes like resistance genes and pathogenesis-related genes can confer broad spectrum and high levels of resistance to several pathogens. Preformed expression of defense is often observed but does not necessarily lead to detrimental effects. Although examples of gene pyramiding are scarce, they suggest that this is a very promising strategy. More field evaluation of the transgenic plants is required to draw final conclusions on the usefulness of these genes for improving disease resistance.

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Discovery of chemically induced mutations in rice by TILLING

Cited by 424 publications

References 44 publications

EMS Induced Mutants of Upland Rice Variety Nagina22: Generation and Characterization

EMS Induced Mutants of Upland Rice Variety Nagina22: Generation and Characterization

Biofortified crops to alleviate micronutrient malnutrition

Potential Candidate Genes for Improving Rice Disease Resistance

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