Abstract:One of the objectives of the cassava-breeding project at CIAT is the identification of clones with special root quality characteristics. A large number of self-pollinations have been made in search of useful recessive traits. During 2006 harvests an S 1 plant produced roots that stained brownish-red when treated with an iodine solution, suggesting that it had lower-than-normal levels of amylose in its starch. Colorimetric and DSC measurements indicated low levels (3.4%) and an absence of amylose in the starch,… Show more
“…Starch is a major component of cassava and has important food and industrial uses (Ceballos et al, 2007). In addition to its widespread application in the food industry, starch is also used in the textile, paper, metallurgy, pharmacy, and plastic industries (Aryee et al, 2006;Emmanuel et al, 2012).…”
ABSTRACT. The aim of this study was to estimate the genetic parameters and predict the genotypic values of root quality traits in cassava (Manihot esculenta Crantz) using restricted maximum likelihood (REML) and best linear unbiased prediction (BLUP). A total of 471 cassava accessions were evaluated over two years of cultivation. The evaluated traits included amylose content (AML), root dry matter (DMC), cyanogenic compounds (CyC), and starch yield (StYi). Estimates of the individual broad-sense heritability of AML were low ( 2 g h = 0.07 ± 0.02), medium for StYi and DMC, and high for CyC. The heritability of AML was substantially improved based on mean of accessions ( 2 m h = 0.28), indicating that some strategies such as increasing the number of repetitions can be used to increase the selective efficiency. In general, the observed genotypic values were very close to the predicted average of the improved population, most likely due to the high accuracy (>0.90), especially for DMC, CyC, and StYi. Gains via selection of the 30 best genotypes for each trait were 4.8 and 3.2% for an increase and decrease for AML, respectively, an increase of 10.75 and 74.62% for DMC for StYi, respectively, and a decrease of 89.60% for CyC in relation to the overall mean of the genotypic values. Genotypic correlations between the quality traits of the cassava roots collected were generally favorable, although they were low in magnitude. The REML/BLUP method was adequate for estimating genetic parameters and predicting the genotypic values, making it useful for cassava breeding.
“…Starch is a major component of cassava and has important food and industrial uses (Ceballos et al, 2007). In addition to its widespread application in the food industry, starch is also used in the textile, paper, metallurgy, pharmacy, and plastic industries (Aryee et al, 2006;Emmanuel et al, 2012).…”
ABSTRACT. The aim of this study was to estimate the genetic parameters and predict the genotypic values of root quality traits in cassava (Manihot esculenta Crantz) using restricted maximum likelihood (REML) and best linear unbiased prediction (BLUP). A total of 471 cassava accessions were evaluated over two years of cultivation. The evaluated traits included amylose content (AML), root dry matter (DMC), cyanogenic compounds (CyC), and starch yield (StYi). Estimates of the individual broad-sense heritability of AML were low ( 2 g h = 0.07 ± 0.02), medium for StYi and DMC, and high for CyC. The heritability of AML was substantially improved based on mean of accessions ( 2 m h = 0.28), indicating that some strategies such as increasing the number of repetitions can be used to increase the selective efficiency. In general, the observed genotypic values were very close to the predicted average of the improved population, most likely due to the high accuracy (>0.90), especially for DMC, CyC, and StYi. Gains via selection of the 30 best genotypes for each trait were 4.8 and 3.2% for an increase and decrease for AML, respectively, an increase of 10.75 and 74.62% for DMC for StYi, respectively, and a decrease of 89.60% for CyC in relation to the overall mean of the genotypic values. Genotypic correlations between the quality traits of the cassava roots collected were generally favorable, although they were low in magnitude. The REML/BLUP method was adequate for estimating genetic parameters and predicting the genotypic values, making it useful for cassava breeding.
“…Water or oil absorption index was calculated as g water or vegetable oil absorbed by 1 g dry sample. Paste clarity was determined according to Ceballos et al (2007), by boiling 1% aqueous solution of starch at 93°C with repeated shaking for 30 min. The solution was cooled and then transferred into a cuvette and transmittance was measured at 650 nm using a spectrophotometer.…”
Section: Characterization Of Physicochemical Properties Of Starchmentioning
Sweet potato starch has limited uses in food industry, but modification of its properties may make it more suitable for use especially for starch based food such as bihon type noodle. The objective of this research was to study the effect of heat moisture treated starch from 4 varieties of sweet potato on the quality of its bihon type noodle. Four different varieties of sweet potato i.e. white, yellow, orange and purple tuber were treated with heat moisture treatment (HMT). The HMT was performed by exposing the starch to high temperature (110°C for 3 hours) at a moisture content of 25%. The HMT modified sweet potato starch was then processed into bihon type noodle. As a control, a commercial bihon type noodle made from rice starch was used. Parameters evaluated were the modified starch and bihon properties. The results showed that the modification of starch with HMT could increase the peak viscosity, setback viscosity, final viscosity and gelatinization temperature but lower the value of the breakdown viscosity. The best bihon from the four varieties of sweet potatoes was that made from white sweet potato starch because it has low cooking losses, low fat, but has high color/brightness and is preferred by panelist.Keywords: bihon, heat moisture treatment, starch, sweet potatoes
ABSTRAK
Keterbatasan pemanfaatan pati alami di dalam industri pangan dapat diatasi dengan cara modifikasi pati sehingga dihasilkan pati dengan karakteristik yang diinginkan terutama pada produk pangan berbahan dasar pati seperti bihun instan. Penelitian ini bertujuan untuk mempelajari pengaruh varietas ubi jalar terhadap karakteristik bihun instan yang dibuat dari pati ubi jalar yang dimodifikasi dengan
“…To date, only two starch mutants have been reported: a natural mutation in a granule-bound starch synthase (gbss) gene resulting in the production of amylose-free starch and a gamma irradiation-induced mutation in an isoamylase gene resulting in high-amylose starch (Ceballos et al, 2007;Ceballos et al, 2008). Because most mutations affecting starch metabolism are recessive, moving useful genetic variants into the appropriate genetic background via traditional breeding is arduous.…”
Several key genes involved in starch biosynthesis have been identified in cassava. However, while phosphorylation has been recognized as an essential step in starch metabolism in higher plants, the underlying gene(s) in cassava have not been isolated so far. To gain insights into starch phosphorylation in cassava, we produced three genomic clones encoding a fragment of an α-glucan, water dikinase (GWD), the primary enzyme required for starch phosphorylation. Sequence analysis showed that each of the clones contained the nucleotide-binding domain of the C-terminus of plant GWDs. The three genomic gwd clones had 98.8% homology at both nucleotide and amino acid levels and represented two distinct allelic sequences with two amino acid substitutions. The shorter clone, pOYE308-1, was 561 base pairs long and encoded a polypeptide of 106 amino acids with a predicted molecular weight of 180.3kDa. Two putative introns were identified in each of the gwd clones. Phylogenetic analysis of the cassava GWD sequences with other plant GWDs revealed that the cassava GWD belongs to the same group as that of castor bean, potato, tomato and tobacco. These resources add to the current knowledge base of starch metabolism in cassava and expand the molecular tool box for starch modification in this important tropical root crop.
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