Discovery, Identification and Comparative Analysis of Non-Specific Lipid Transfer Protein (nsLtp) Family in Solanaceae

Abstract

Plant non-specific lipid transfer proteins (nsLtps) have been reported to be involved in plant defense activity against bacterial and fungal pathogens. In this study, we identified 135 (122 putative and 13 previously identified) Solanaceae nsLtps, which are clustered into 8 different groups. By comparing with Boutrot’s nsLtp classification, we classified these eight groups into five types (I, II, IV, IX and X). We compared Solanaceae nsLtps with Arabidopsis and Gramineae nsLtps and found that (1) Types I, II and IV are shared by Solanaceae, Gramineae and Arabidopsis; (2) Types III, V, VI and VIII are shared by Gramineae and Arabidopsis but not detected in Solanaceae so far; (3) Type VII is only found in Gramineae whereas type IX is present only in Arabidopsis and Solanaceae; (4) Type X is a new type that accounts for 52.59% Solanaceae nsLtps in our data, and has not been reported in any other plant so far. We further built and compared the three-dimensional structures of the eight groups, and found that the major functional diversification within the nsLtp family could be predated to the monocot/dicot divergence, and many gene duplications and sequence variations had happened in the nsLtp family after the monocot/dicot divergence, especially in Solanaceae.

Genomic Identification and Comparative Expansion Analysis of the Non-Specific Lipid Transfer Protein Gene Family in Gossypium

“…S1). It has been speculated that Type VII nsLTPs appeared specifically in monocots while Type IX nsLTPs were unique to dicots39, and our results in Gossypium further confirmed this viewpoint. In addition, G. arboreum lost all the Type III and Type IX nsLTPs (Fig.…”

“…The system categorized 267 nsLTPs into nine types (Type I-IX) based on a genome-wide analysis of rice ( Oryza sativa ), wheat ( Triticum aestivum ) and Arabidopsis thaliana . Then, nsLTPs in other plant species were also grouped according to Boutrot’s method with slight modifications49101112, and novel types such as Type X9 and Type XI4 were identified.…”

Isoform identification, recombinant production and characterization of the allergen lipid transfer protein 1 from pear (Pyr c 3)

“…This analysis allowed us to identify 4 different sequences, named LTPa, LTPb, LTPc and LTPd, which were found 3, 2, 1 and 2 times, respectively. This result is consistent with LTP1 being encoded by multiple genes, as previously reported (Botton et al, 2002;Gao et al, 2005;Liu et al, 2010;Vignols et al, 1997). In Fig.…”

Genome-Wide Survey and Expression Analysis of the Putative Non-Specific Lipid Transfer Proteins in Brassica rapa L

“…Moreover, we found that only one type VIII and very few type III/V/VI nsLtp genes existed in B. rapa (Table 1), just like those in Arabidopsis and rice [17]. A previous study demonstrated that four of the types (III/V/VI/VIII) were not identified in six Solanaceae species thus far [18]. Our results also showed that type V/VIII BrnsLtps had no duplications (Fig.…”