Isoform identification, recombinant production and characterization of the allergen lipid transfer protein 1 from pear (Pyr c 3)

Expression and characterization of a new isoform of the 9 kDa allergenic lipid transfer protein from tomato (variety San Marzano)

“…It results in an emission curve (registered in the 280e400 nm range) with a maximum at 305 nm (Supplementary material, Fig. 3S), similar to those observed for other LTPs (Ramazzina et al, 2012). In Fig.…”

Electrospray MS and MALDI imaging show that non‐specific lipid‐transfer proteins (LTPs) in tomato are present as several isoforms and are concentrated in seeds

“…As shown, all the four proteins had a molecular weight close to the one already found for other plant LTPs, such as Pru P 3 from peach (9135 Da), LTPa and LTPb from pear (9252 and 9250 Da), LTPa and LTPb from pomegranate (9342 and 9467 Da), LTP from maize (9046 Da), and Mal d 3 from apple (9076 Da) . Quite interestingly, all the four potential tomato LTP isoforms co‐eluted in two chromatographic peaks (as shown in Fig.…”

Identification of gibberellin‐regulated protein as a new allergen in orange allergy

“…Previous reports also indicated a lack of correlation between IgE reactivity for purified protein and patient symptoms, possibly caused by differences between the methodologies used. Ramazzina et al reported a study of pear allergy where IgE ELISA demonstrated considerable immunoreactivity of allergens to patient sera, although allergic reactions after the ingestion of pears were not reported in the clinical history of the patients . In addition, Ebo et al reported the enhanced sensitivity and specificity of BAT for the diagnosis of oral allergy syndrome caused by apple .…”

“…Previous reports also indicated a lack of correlation between IgE allergic reactions after the ingestion of pears were not reported in the clinical history of the patients. 31 In addition, Ebo et al reported the enhanced sensitivity and specificity of BAT for the diagnosis of oral allergy syndrome caused by apple. 32 Comparison between subjects with oral allergy syndrome caused by apple and healthy controls showed sensitivities and specificities of 96% and 100% for apple IgE and 88% and 100% for the apple SPT, respectively.…”