Discovery and Characterization of Novel MicroRNAs During Endothelial Differentiation of Human Embryonic Stem Cells

Yoo, Jung Ki; Kim, Jumi; Choi, Seong–Jun; Noh, Hye Min; Kwon, Young Do; Yoo, Hai-Soo; Yi, Hyo seon; Chung, Hyung Min; Kim, Jin Kyeoung

doi:10.1089/scd.2011.0500

Cited by 70 publications

(48 citation statements)

References 46 publications

(54 reference statements)

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“…The miRNAs were cloned into vectors using the Dyna Express miRNA Cloning Kit (BioDynamics Laboratory Inc., Tokyo, Japan), according to the manufacturer's instructions with some modifications. 48,49 Transfection and mimics. The cell transfections were performed using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA), according to the manufacturer's protocol.…”

Section: Methodsmentioning

confidence: 99%

The novel miR-9500 regulates the proliferation and migration of human lung cancer cells by targeting Akt1

Jung

Lee

et al. 2014

Cell Death Differ

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MicroRNAs have crucial roles in lung cancer cell development. They regulate cell growth, proliferation and migration by mediating the expression of tumor suppressor genes and oncogenes. We identified and characterized the novel miR-9500 in human lung cancer cells. The miR-9500 forms a stem-loop structure and is conserved in other mammals. The expression levels of miR-9500 were reduced in lung cancer cells and lung cancer tissues compared with normal tissues, as verified by TaqMan miRNA assays. It was confirmed that the putative target gene, Akt1, was directly suppressed by miR-9500, as demonstrated by a luciferase reporter assay. The miR-9500 significantly repressed the protein expression levels of Akt1, as demonstrated via western blot, but did not affect the corresponding mRNA levels. Akt1 has an important role in lung carcinogenesis, and depletion of Akt1 has been shown to have antiproliferative and anti-migratory effects in previous studies. In the current study, the overexpression of miR-9500 inhibited cell proliferation and the expression of cell cycle-related proteins. Likewise, the overexpression of miR-9500 impeded cell migration in human lung cancer cells. In an in vivo assay, miR-9500 significantly suppressed Fluc expression compared with NC and ASO-miR-9500, suggesting that cell proliferation was inhibited in nude mice. Likewise, miR-9500 repressed tumorigenesis and metastasis by targeting Akt1. These data indicate that miR-9500 might be applicable for lung cancer therapy.

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Section: Methodsmentioning

confidence: 99%

The novel miR-9500 regulates the proliferation and migration of human lung cancer cells by targeting Akt1

Jung

Lee

et al. 2014

Cell Death Differ

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“…A single miRNA is able to regulate hundreds of different mRNA targets in various cellular functions, including metabolism, proliferation, apoptosis, senescence, and differentiation [60]. More than 45 miRNAs have been associated to replicative senescence of human MSCs [61][62][63]. The let-7 family of miRNAs and miRNAsbinding protein Argonaute 2 (AGO2) are critical for senescence induction by facilitating transcriptional gene silencing of E2F-regulated proliferation promoting genes [61,64].…”

Section: Non-coding Rnas Affect Senescence-from Short To Long Transcrmentioning

confidence: 99%

Epigenetic Modifications upon Senescence of Mesenchymal Stem Cells

2016

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Cellular senescence is a continuous and highly organized process that alters the intricate genomic network in order to maintain cellular homeostasis. It occurs in all primary cell cultures-including mesenchymal stem cells (MSCs), which are concurrently tested for a wide variety of clinical applications. Differentiation potential as well as paracrine secretion of MSCs is severely affected by cellular senescence. There is a growing perception that nuclear reorganization and epigenetic modifications contribute to trigger and maintain functional differences in long-term culture. In this review, we discuss molecular and epigenetic aspects that evoke functional changes in cellular aging-indicating that the underlying process is not only an accumulation of cellular defects, but rather epigenetically orchestrated.

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“…Among them, miR-5739 and miR-6087 modulate the expression of endoglin [139,140], miR-6078 targets the E-cadherin (Cdh) gene [140], while miR-7641 suppresses expression of CXCL1 [141].…”

Section: Other Mirnasmentioning

confidence: 99%

An updated view on the differentiation of stem cells into endothelial cells

Zhou

Yang

Chen

et al. 2014

Sci. China Life Sci.

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Endothelial cells form the internal barrier between circulating blood and the vessel wall. They regulate arterial activity and mediate pathological reactions to vascular injuries such as atherosclerosis and balloon angioplasty. The development and differentiation of endothelial cells is a complex and coordinated process involving multiple levels of signaling and transcriptional and post-transcriptional regulation. Elucidating the mechanism of endothelial differentiation will not only enhance our understanding of vascular disease pathogenesis, but also facilitate our ability to produce vessels cells from pluripotent stem cells for regeneration purposes. In this review, we discuss the current understanding of how stem cells differentiate into endothelial cells at the level of signaling, transcription and microRNA regulation.

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Discovery and Characterization of Novel MicroRNAs During Endothelial Differentiation of Human Embryonic Stem Cells

Cited by 70 publications

References 46 publications

The novel miR-9500 regulates the proliferation and migration of human lung cancer cells by targeting Akt1

The novel miR-9500 regulates the proliferation and migration of human lung cancer cells by targeting Akt1

Epigenetic Modifications upon Senescence of Mesenchymal Stem Cells

An updated view on the differentiation of stem cells into endothelial cells

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