Discovery and characterization of miRNA during cellular senescence in bone marrow-derived human mesenchymal stem cells

Yoo, Jung Ki; Kim, Chang‐Hyun; Jung, Ho Yong; Lee, Dong Ryul; Kim, Jin Kyeoung

doi:10.1016/j.exger.2014.07.020

Cited by 40 publications

(30 citation statements)

References 47 publications

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“…Induction of premature senescence in HDFs was accompanied by upregulation of p16 INK4a ; an important downstream target of BMI1 and a major regulator of senescence [23]. It has been reported that miR-141-3p, miR-10a and miR-199b-5p regulate senescence in human mesenchymal stem cells by targeting ZMPSTE24, KLF4 or LAMC networks, respectively [24][25][26]. Thus, we focused on miRNA as a candidate regulator of ROCK1 during TSPC aging.…”

Section: Discussionmentioning

confidence: 99%

miR-135a modulates tendon stem/progenitor cell senescence via suppressing ROCK1

Chen

Wang

Liu

et al. 2015

Bone

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Section: Discussionmentioning

confidence: 99%

miR-135a modulates tendon stem/progenitor cell senescence via suppressing ROCK1

Chen

Wang

Liu

et al. 2015

Bone

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“…MicroRNAs (miRNAs) play important roles in the regulation of cellular senescence [23, 24] and the pathogenesis of PE [25]. MiR-495 was reported as an anti-tumor miRNA which could inhibit cell migration, invasion and proliferation, thus to play negative roles in the carcinogenesis [26-29].…”

Section: Introductionmentioning

confidence: 99%

MiR-495 Promotes Senescence of Mesenchymal Stem Cells by Targeting Bmi-1

Song

Liú

et al. 2017

Cell Physiol Biochem

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Background/Aims: Mesenchymal stem cells (MSCs) play an important role in regulating angiogenesis and immune balance. Abnormal proliferation and function of MSCs were reported at maternal fetal interface in patients with pre-eclampsia (PE). Micro-RNA-495 was known to be upregulated in the MSCs derived from patients with PE. However, it is not clear whether the up-regulated miR-495 is related to the pathogenesis of PE. Methods: We analyzed the expression of miR-495 in MSCs and umbilical cords derived from healthy pregnancies (NC) and PE, then we upregulated or downregulated the expression of miR-495 in MSCs derived from NC and tested the proliferation, apoptosis, migration, invasion, tube formation and senescence. Results: In the current study, we found that the expression of miR-495 was significantly increased in both umbilical cord tissues and MSCs in patients with severe PE. Overexpressing miR-495 arrested cell cycle in S phase and promoted cell apoptosis. The supernatants from miR-495-overexpressed-MSCs inhibited the migration of MSCs and HTR-8/SVneo, invasion of HTR-8/SVneo and tube formation of HUVEC, while si-miR-495 had the opposite effects. Furthermore, we analyzed the senescence related β-galactosidase activity and CD146 and found that miR-495 induced the senescence of MSCs. Molecular mechanism studies confirmed that Bmi-1 mediated these effects of miR-495 on MSCs. Conclusion: Taken together, our data demonstrated that miR-495 induced senescence of MSCs may be involved in the pathogenesis of PE.

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“…A large number of studies have shown that miR‐330‐5p plays an important role in the cell life cycle (Yoo, Kim, Jung, Lee, & Kim, ). Therefore, we speculate that miR‐330‐5p may begin to participate in other important life processes during the middle and later stages of differentiation, resulting in the ectopic expression of miR‐330‐5p.…”

Section: Discussionmentioning

confidence: 99%

MiR‐330‐5p negatively regulates ovine preadipocyte differentiation by targeting branched‐chain aminotransferase 2

Shi

Yan

Qiao

et al. 2018

Animal Science Journal

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The differentiation of preadipocytes into adipose tissues is tightly regulated by various factors including microRNAs and cytokines. This article aims to study the effect of miR-330-5p on expression of BCAT2 in ovine preadipocytes. Ovine preadipocytes were isolated, and we found that the miR-330-5p expression decreased gradually during the early differentiation of ovine preadipocytes, while BCAT2 expression increased. BCAT2 was identified as a direct target of miR-330-5p, ectopic expression of miR-330-5p could change the expression of both BCAT2 mRNA and protein. Silencing BCAT2 had the same inhibition effects as overexpressing miR-330-5p on the preadipocyte differentiation, but overexpressing BCAT2 had the converse effects. Taken together, we demonstrated that miR-330-5p is a negative regulator of differentiation by targeting BCAT2, and clarified the role of BCAT2 and miR-330-5p during preadipocyte differentiation.

show abstract

Discovery and characterization of miRNA during cellular senescence in bone marrow-derived human mesenchymal stem cells

Cited by 40 publications

References 47 publications

miR-135a modulates tendon stem/progenitor cell senescence via suppressing ROCK1

miR-135a modulates tendon stem/progenitor cell senescence via suppressing ROCK1

MiR-495 Promotes Senescence of Mesenchymal Stem Cells by Targeting Bmi-1

MiR‐330‐5p negatively regulates ovine preadipocyte differentiation by targeting branched‐chain aminotransferase 2

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