Discharge properties of mitral/tufted cells in the olfactory bulb of cats

Motokizawa, Fumiaki; Ogawa, Yoichi

doi:10.1016/s0006-8993(97)00511-8

Cited by 8 publications

(3 citation statements)

References 12 publications

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“…Similar long bursts had previously been described in the miniature pig (Reinhardt et al. ), and similar bursts were later described in cats anesthetized with an initial dose of ketamine hydrochloride and maintained under anesthesia with intravenous urethane–chloralose (Motokizawa and Ogawa ). These authors also reported another feature of mitral cell activity for the first time – that the distribution of interspike intervals was often bimodal, with an early mode that reflects the frequent occurrence of spikes separated by very short intervals (<10 msec).…”

Section: Introductionsupporting

confidence: 78%

Discharge patterning in rat olfactory bulb mitral cells in vivo

et al. 2014

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Here we present a detailed statistical analysis of the discharge characteristics of mitral cells of the main olfactory bulb of urethane‐anesthetized rats. Neurons were recorded from the mitral cell layer, and antidromically identified by stimuli applied to the lateral olfactory tract. All mitral cells displayed repeated, prolonged bursts of action potentials typically lasting >100 sec and separated by similarly long intervals; about half were completely silent between bursts. No such bursting was observed in nonmitral cells recorded in close proximity to mitral cells. Bursts were asynchronous among even adjacent mitral cells. The intraburst activity of most mitral cells showed strong entrainment to the spontaneous respiratory rhythm; similar entrainment was seen in some, but not all nonmitral cells. All mitral cells displayed a peak of excitability at ~25 msec after spikes, as reflected by a peak in the interspike interval distribution and in the corresponding hazard function. About half also showed a peak at about 6 msec, reflecting the common occurrence of doublet spikes. Nonmitral cells showed no such doublet spikes. Bursts typically increased in intensity over the first 20–30 sec of a burst, during which time doublets were rare or absent. After 20–30 sec (in cells that exhibited doublets), doublets occurred frequently for as long as the burst persisted, in trains of up to 10 doublets. The last doublet was followed by an extended relative refractory period the duration of which was independent of train length. In cells that were excited by application of a particular odor, responsiveness was apparently greater during silent periods between bursts than during bursts. Conversely in cells that were inhibited by a particular odor, responsiveness was only apparent when cells were active. Extensive raw (event timing) data from the cells, together with details of those analyses, are provided as supplementary material, freely available for secondary use by others.

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Section: Introductionsupporting

confidence: 78%

Discharge patterning in rat olfactory bulb mitral cells in vivo

et al. 2014

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“…Olfactory bulb circuits generate robust spontaneous spike activity in the resting, unstimulated state (Walsh, 1956;Chaput and Holley, 1979;Doving, 1987;Wellis et al, 1989;Motokizawa and Ogawa, 1997;Kay and Laurent, 1999;Rinberg et al, 2006). Spontaneous spiking is also strongly manifested in olfactory bulb neurons in vitro (Isaacson, 1999;Carlson et al, 2000) being driven by intrinsic pacemaker action in specific cell types (Hayar et al, 2004;Puopolo et al, 2005).…”

Section: Nitric Oxide Gradients In Mouse Olfactory Bulb Slices In Vitromentioning

confidence: 99%

Tonic and stimulus-evoked nitric oxide production in the mouse olfactory bulb

Lowe

Buerk

et al. 2008

Neuroscience

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Nitric oxide (NO) has been long assumed to play a key role in mammalian olfaction. This was based largely on circumstantial evidence, i.e. prominent staining for nitric oxide synthase (NOS) and cyclic GMP or soluble guanylyl cyclase, an effector enzyme activated by NO, in local interneurons of the olfactory bulb. Here we employ innovative custom-fabricated NO micro-sensors to obtain the first direct, time-resolved measurements of NO signaling in the olfactory bulb. In 400 μm thick mouse olfactory bulb slices, we detected a steady average basal level of 87 nM NO in the extracellular space of mitral or granule cell layers. This NO 'tone' was sensitive to NOS substrate manipulation (200 μM L-arginine, 2 mM L-NAME) and Mg 2+ modulation of NMDA receptor conductance. Electrical stimulation of olfactory nerve fibers evoked transient (peak at 10 s) increments in NO levels 90 -100 nM above baseline. In the anesthetized mouse, NO micro-sensors inserted into the granule cell layer detected NO transients averaging 55 nM in amplitude and peaking at 3.4 sec after onset of a 5 sec odorant stimulation. These findings suggest dual roles for NO signaling in the olfactory bulbtonic inhibitory control of principal neurons, and regulation of circuit dynamics during odor information processing.

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“…In addition, even in the absence of such background stimuli, many types of neurons exhibit spontaneous activity. For example, mitral cells in the mammalian olfactory bulb show patterned spontaneous activity in the absence of odor stimuli 2 , 3 . This spontaneous activity could also be regarded as a type of prior activity.…”

Section: Introductionmentioning

confidence: 99%

Prior activity of olfactory receptor neurons is required for proper sensory processing and behavior in Drosophila larvae

Utashiro

Williams

Parrish

et al. 2018

Sci Rep

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Animal responses to their environment rely on activation of sensory neurons by external stimuli. In many sensory systems, however, neurons display basal activity prior to the external stimuli. This prior activity is thought to modulate neural functions, yet its impact on animal behavior remains elusive. Here, we reveal a potential role for prior activity in olfactory receptor neurons (ORNs) in shaping larval olfactory behavior. We show that prior activity in larval ORNs is mediated by the olfactory receptor complex (OR complex). Mutations of Orco, an odorant co-receptor required for OR complex function, cause reduced attractive behavior in response to optogenetic activation of ORNs. Calcium imaging reveals that Orco mutant ORNs fully respond to optogenetic stimulation but exhibit altered temporal patterns of neural responses. These findings together suggest a critical role for prior activity in information processing upon ORN activation in Drosophila larvae, which in turn contributes to olfactory behavior control.

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Discharge properties of mitral/tufted cells in the olfactory bulb of cats

Cited by 8 publications

References 12 publications

Discharge patterning in rat olfactory bulb mitral cells in vivo

Discharge patterning in rat olfactory bulb mitral cells in vivo

Tonic and stimulus-evoked nitric oxide production in the mouse olfactory bulb

Prior activity of olfactory receptor neurons is required for proper sensory processing and behavior in Drosophila larvae

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