Directional Changes in the Intestinal Bacterial Community in Black Soldier Fly (Hermetia illucens) Larvae

Li, Xinfu; Zhou, Shen; Zhang, Jing; Zhou, Zhihao; Xiong, Qiang

doi:10.3390/ani11123475

Cited by 13 publications

(14 citation statements)

References 62 publications

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“…to new resource patches and increase their abundance in the environment. We must also determine whether the most important factor affecting fungal composition is the insect diet ( Li et al 2021 ) or the environmental composition ( Cohen et al 2020 ). It seems that in the locations that were not dominant by the Candida (MA and KH) the fungal community composition was influenced by the fiber content of the compost.…”

Section: Discussionmentioning

confidence: 99%

Assessing Fungal Diversity and Abundance in the Black Soldier Fly and its Environment

Vitenberg

Opatovsky

2022

Journal of Insect Science

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Detritivorous insects that flourish in decaying environments encounter microorganisms throughout their life cycle. However, it is not clear whether the microbial composition of the decaying environment affects the microbial composition of the insect gut, or whether the opposite is true, with the microorganisms that are adapted to the insect’s digestive system being dispersed by the insects to new habitats, thereby becoming more and more common in the environment. To test these questions the fungal composition of the black soldier fly (BSF) (Stratiomyidae; Hermetia illucens Linnaeus) larval gut and its surrounding decaying environment (household compost bins) were analyzed using amplicon sequencing. Constancy in the dominance of the genus Candida (Debaryomycetaceae) in most of the environments and larval guts was found. This finding may suggest a ‘core’ structure to the fungal community of the BSF. In locations where nutrient composition of the environment had higher fiber content, the Candida was not dominant and the most common fungi were the genus Gibberella (Nectriaceae) and the family Dipodascaceae. The later was dominant also in the larval gut and the former was replaced with Meyerozyma (Debaryomycetaceae), which may suggest a selection process by the insect’s gut. Little is known about the ecological interactions of insects with eukaryotic microorganisms, such as yeast-like fungi. As their metabolic complexity and ability is intense, they have the potential to dramatically affect the physiological condition of the insect.

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Section: Discussionmentioning

confidence: 99%

Assessing Fungal Diversity and Abundance in the Black Soldier Fly and its Environment

Vitenberg

Opatovsky

2022

Journal of Insect Science

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“…to new resource patches and increase their abundance in the environment. We must also determine whether the most important factor affecting fungal composition is the insect diet (22) or the environmental composition (23). It seems that in the two locations that were not dominated by Candida , KH and MA, the fungal community composition was influenced by the fiber content of the compost.…”

Section: Discussionmentioning

confidence: 99%

The connection between the gut mycobiome community of natural black soldier fly populations and their environment

Vitenberg¹,

Martin²,

Opatovsky³

2022

Preprint

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In contrast to interactions between prokaryotic organisms (e.g. bacteria) and insects, an understanding of the interactions between eukaryotic microorganisms (e.g. fungi) and insects is lacking. In this study, black soldier fly larvae were collected from natural populations in household composts. The mycobiome composition of the larval gut and environment was identified using next generation sequencing. The nutrient composition of the larvae's environment was analyzed. The mycobiome composition was divided into three groups: the first included larvae from Kfar HaHoresh that comprised mostly Meyerozyma and were influenced by the fiber and carbohydrate composition of the compost; the second included the compost samples from Kfar HaHoresh and Misgav Am that mainly comprised the Dipodascaceae family and were influenced by the fiber composition of the compost. The third group contained all the remaining compost and larvae samples and mainly comprised the genus Candida. Candida spp. are the most common in the environment and may thus affect their abundance in the insect gut. However, this species may be adapted to the insect’s gut, providing the insect with a benefit that allows the insect to disperse Candida to new resource patches and thereby help increase its abundance in the environment. In cases where dominant species are not present, it seems that the important factor determining fungal composition in the insect gut is its diet. Understanding the metabolic effect of fungi, e.g. Candida, on the BSF larvae is still a black box which requires further study.

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“…In these studies, some genera, such as Proteus and Enterococcus , were frequently cultured regardless of the set-up; however, some genera, for instance Serratia and Rhodococcus , were isolated exclusively in one particular study, indicating that the isolation process influences the obtained strain collection. After isolation of potential probiotics, some studies have characterized their strain collection on phenotypic properties potentially relevant for in vivo activity, such as enzyme activity, production of exopolysaccharides, and carbon utilization, to confer ecological advantages to the host [ 38 , 43 , 46 ]. Besides these metabolic screening tests, Savio et al [ 30 ] listed a set of in vitro and in vivo screening techniques to characterize the probiotic potential of candidate strains for application in mass-reared insects environments such as tests focusing on adhesion properties.…”

Section: Origin Of the Probioticsmentioning

confidence: 99%

“…1069/2009 [ 56 ]. Interestingly, feeding substrates were autoclaved in a few studies [ 41 , 43 , 47 ], probably to support colonization of the probiotics in the diets as this method would limit competition with other microorganisms. Yet, these sterilized substrates also resulted in a slower development of BSFL [ 23 , 47 , 57 ].…”

Section: Set-up Of Inoculation Trials For Bsfl Rearingmentioning

confidence: 99%

The Use of Probiotics during Rearing of Hermetia illucens: Potential, Caveats, and Knowledge Gaps

2023

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Given the novelty of the industrial production of the edible insects sector, research has primarily focused on the zootechnical performances of black soldier fly larvae (BSFL) in response to different substrates and rearing conditions as a basis to optimize yield and quality. However recently, research has started to focus more on the associated microbes in the larval digestive system and their substrates and the effect of manipulating the composition of these communities on insect performance as a form of microbiome engineering. Here we present an overview of the existing literature on the use of microorganisms during rearing of the BSFL to optimize the productivity of this insect. These studies have had variable outcomes and potential explanations for this variation are offered to inspire future research that might lead to a better success rate for microbiome engineering in BSFL.

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Directional Changes in the Intestinal Bacterial Community in Black Soldier Fly (Hermetia illucens) Larvae

Cited by 13 publications

References 62 publications

Assessing Fungal Diversity and Abundance in the Black Soldier Fly and its Environment

Assessing Fungal Diversity and Abundance in the Black Soldier Fly and its Environment

The connection between the gut mycobiome community of natural black soldier fly populations and their environment

The Use of Probiotics during Rearing of Hermetia illucens: Potential, Caveats, and Knowledge Gaps

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