The Use of Probiotics during Rearing of Hermetia illucens: Potential, Caveats, and Knowledge Gaps

Gorrens, E.; Lecocq, Antoine; Smet, Jeroen De

doi:10.3390/microorganisms11020245

Cited by 5 publications

(3 citation statements)

References 82 publications

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“…The development of H. illucens larvae can be affected by various extrinsic factors such as temperature, gut microbes, food nutrition, and xenobiotics [ 23 , 54 , 55 ]. Intrinsic factors, such as the expression level of metabolic genes, also perform vital roles during the developmental process.…”

Section: Discussionmentioning

confidence: 99%

An Acyl Carrier Protein Gene Affects Fatty Acid Synthesis and Growth of Hermetia illucens

Peng

Liu

et al. 2023

Insects

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Acyl carrier protein (ACP) is an acyl carrier in fatty acid synthesis and is an important cofactor of fatty acid synthetase. Little is known about ACP in insects and how this protein may modulate the composition and storage of fatty acids. We used an RNAi-assisted strategy to study the potential function of ACP in Hermetia illucens (Diptera: Stratiomyidae). We identified a HiACP gene with a cDNA length of 501 bp and a classical conserved region of DSLD. This gene was highly expressed in the egg and late larval instars and was most abundant in the midgut and fat bodies of larvae. Injection of dsACP significantly inhibited the expression level of HiACP and further regulated the fatty acid synthesis in treated H. illucens larvae. The composition of saturated fatty acids was reduced, and the percentage of unsaturated fatty acids (UFAs) was increased. After interfering with HiACP, the cumulative mortality of H. illucens increased to 68.00% (p < 0.05). H. illucens growth was greatly influenced. The development duration increased to 5.5 days, the average final body weights of larvae and pupae were decreased by 44.85 mg and 14.59 mg, respectively, and the average body lengths of larvae and pupae were significantly shortened by 3.09 mm and 3.82 mm, respectively. The adult eclosion rate and the oviposition of adult females were also severely influenced. These results demonstrated that HiACP regulates fatty acid content and influences multiple biological processes of H. illucens.

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Section: Discussionmentioning

confidence: 99%

An Acyl Carrier Protein Gene Affects Fatty Acid Synthesis and Growth of Hermetia illucens

Peng

Liu

et al. 2023

Insects

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“…Gram-positive Bacillus, Enterococcus, Lactobacillus, Lactococcus, Pediococcus, and Streptococcus bacterial strains, as well as the yeasts Saccharomyces cerevisiae and Kluyveromyces, are the most used probiotic agents in feed supplements in the European Union (Anadón et al, 2006;Gorrens et al, 2023;.…”

Section: Probiotic Applications In Mass-rearing Environmentsmentioning

confidence: 99%

“…Among the microorganisms with potential probiotic activities for insects, Bacilli, Gammaproteobacteria and Actinomyceta have been proposed for improving the quality and the efficiency of waste conversion of Hermetia illucens (Gorrens et al, 2023).…”

Section: Probiotic Applications In Mass-rearing Environmentsmentioning

confidence: 99%

Effects of feed and probiotic bacteria on Tenebrio molitor performance and entomopathogen susceptibility

Savio

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Lactobacillus plantarum is available in several probiotic products due to its proven abilities in reducing inflammatory bowel disease, the irritable bowel syndrome and preventing diarrhea.Application to Diptera and Lepidoptera showed effects of Lb. plantarum on host development and performance raising questions about application to other insect Orders. Research objectives and outline of this thesisThe purpose of this thesis is to provide a broad overview of T. molitor symbionts, conduct experimental studies focused on potential application of beneficial symbionts such as Pediococcus pentosaceus KVL B19-01 and Lactobacillus plantarum WJB as potential probiotic strains for decreasing the risk of infections in mass-rearing conditions and for optimizing the quality of the chemical composition of the larvae as they may be consumed as feed and food, representing one of the solutions for upscaling the value of by-products along the food chain.Chapter 2 provides a review of the potential of probiotic applications in mass-reared insects. Methods of isolation, identification and production of probiotic bacterial species are provided, taking into consideration methods of their application in an insect mass-rearing context and the important connections between host-microbiota and provided feed in health preservation and disease management.Chapter 3, provides a review addressing the richness and the variety of interactions between the yellow mealworm and its symbionts, methods of pathogen detection and insights for reducing the risk of pathogen infection in rearing facilities.Chapter 4, is an experimental study focused on T. molitor susceptibility to the entomopathogen Bacillus thuringiensis sv. morrisoni biovar tenebrionis (4AA1) (Btt) in conditions resembling the mass-rearing system environment. The study highlights the direct correlation between entomopathogen load and the yellow mealworm larval growth and mortality. Larvae having higher body mass showed to be more resistant to the pathogen and were not compromised in feed conversion. The data also showed that Btt can persist in the tested conditions suggesting a certain risk for the rearing.Chapter 5, focus on the effects of diet composition and two potential probiotic strains, Pediococcus pentosaceus KVL B19-01 and Lactobacillus plantarum WJB, on performance and microbial community composition of T. molitor larvae affected by the entomopathogens Metarhizium brunneum KVL 12-30 and Bacillus thuringiensis sv. morrisoni biovar tenebrionis (4AA1) single or co-infection.The larvae presented increased growth and survival in case of co-infection when fed with diet supplemented with P. pentosaceus. The bacterial community composition of the larvae was similar in all treatments, indicating that the probiotic strain did not establish at a detectable level in the insect, however, its transient presence improved larval performance in its early developmental stages.In Chapter 6, the effects of diet composition and probiotic provision on T. molitor larval performance and chemical com...

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Framework for valorizing waste- and by-products through insects and their microbiomes for food and feed

Muurmann,

Banovic,

Gilbert

et al. 2024

Food Research International

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The Use of Probiotics during Rearing of Hermetia illucens: Potential, Caveats, and Knowledge Gaps

Cited by 5 publications

References 82 publications

An Acyl Carrier Protein Gene Affects Fatty Acid Synthesis and Growth of Hermetia illucens

An Acyl Carrier Protein Gene Affects Fatty Acid Synthesis and Growth of Hermetia illucens

Effects of feed and probiotic bacteria on Tenebrio molitor performance and entomopathogen susceptibility

Framework for valorizing waste- and by-products through insects and their microbiomes for food and feed

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