Directed Evolution of Aptamer Discovery Technologies

Wu, Diana; Gordon, Chelsea K. L.; Shin, John H; Eisenstein, Michael; Soh, H. Tom

doi:10.1021/acs.accounts.1c00724

Cited by 38 publications

(31 citation statements)

References 68 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although aptamers have distinct advantages over Abs, their availability for various receptors is limited compared to Abs. Generating highly specific aptamers has proven challenging with conventional processes like SELEX (Systematic Evolution of Ligands by EXponential Enrichment) . Modern high-throughput screening platforms will be required to design and select aptamers for epitopes of multiplexed protein targets with optimal binding affinities. , …”

Section: Future Design Opportunities For Digital Ev Assaysmentioning

confidence: 99%

Future of Digital Assays to Resolve Clinical Heterogeneity of Single Extracellular Vesicles

Morales

2022

ACS Nano

View full text Add to dashboard Cite

Extracellular vesicles (EVs) are complex lipid membrane vehicles with variable expressions of molecular cargo, composed of diverse subpopulations that participate in the intercellular signaling of biological responses in disease. EV-based liquid biopsies demonstrate invaluable clinical potential for overhauling current practices of disease management. Yet, EV heterogeneity is a major needle-in-a-haystack challenge to translate their use into clinical practice. In this review, existing digital assays will be discussed to analyze EVs at a single vesicle resolution, and future opportunities to optimize the throughput, multiplexing, and sensitivity of current digital EV assays will be highlighted. Furthermore, this review will outline the challenges and opportunities that impact the clinical translation of single EV technologies for disease diagnostics and treatment monitoring.

show abstract

Section: Future Design Opportunities For Digital Ev Assaysmentioning

confidence: 99%

Future of Digital Assays to Resolve Clinical Heterogeneity of Single Extracellular Vesicles

Morales

2022

ACS Nano

View full text Add to dashboard Cite

show abstract

“…The last decade has witnessed the great progress in the development of aptasensors for malathion monitoring, which are usually coupled with colorimetric (Bala et al, 2016;Bala et al, 2017), fluorescent (Bala et al, 2018;Huang et al, 2021), electrochemical (Xu et al, 2019;Xu et al, 2021b), and chemiluminescent (Wu et al, 2021) techniques. Colorimetric detection methods have attracted special attention due to the instrument-free, easy-to-use, and low-cost features (Guo et al, 2021;Wu et al, 2022). It is thus of great importance to develop simple yet robust methods for malathion analysis.…”

Section: Introductionmentioning

confidence: 99%

Bio-inspired aptamers decorated gold nanoparticles enable visualized detection of malathion

Zhan

Tao

Xie

et al. 2023

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Biosensors always respond to the targets of interest in a specific manner, employing biological or bio-mimic recognition elements such as antibodies and aptamers. Inspired by target recognition in nature, an aptamer-mediated, gold nanoparticle-based sensing approach is developed in this work for effective determination of malathion. The sensing system consists of negatively charged aptamer probes, and polycationic proteins, protamine, as well as exceptional colorimetric nanoprobes, barely gold nanoparticles (AuNPs). Protamine molecules bound to aptamer probes hinder the aggregation of AuNPs, while no such inhibition is maintained when aptamer-specific malathion is introduced into the solution, thus leading to the solution colour change from red to blue observable by the naked eye. The assay is accomplished via a mix-and-measure step within 40 min with a detection limit as low as 1.48 μg/L (3σ/s rule). The assay method also exhibits high selectivity and good applicability for the quantification of malathion in tap water with recovery rates of 98.9%–109.4%. Additionally, the good detection accuracy is also confirmed by the high-performance liquid chromatography method. Therefore, the non-enzymatic, label- and device-free characteristics make it a robust tool for malathion assay in agricultural, environmental, and medical fields.

show abstract

“…Most aptamers were isolated in vitro from randomized libraries by systematic evolution of ligands by exponential enrichment (SELEX). [17][18][19] They can bind to a variety of targets with excellent specificity and affinity. DNA aptamers offer many advantages in biosensing, including high stability, programmable structures, and ease of incorporation of versatile signal reporting probes.…”

Section: Introductionmentioning

confidence: 99%

“…Aptamers are single‐stranded oligonucleotides that can selectively bind to target molecules. Most aptamers were isolated in vitro from randomized libraries by systematic evolution of ligands by exponential enrichment (SELEX) [17–19] . They can bind to a variety of targets with excellent specificity and affinity.…”

Section: Introductionmentioning

confidence: 99%

Salt‐Toggled Capture Selection of Uric Acid Binding Aptamers

Liu

2022

ChemBioChem

View full text Add to dashboard Cite

Uric acid is the end‐product of purine metabolism in humans and an important biomarker for many diseases. To achieve the detection of uric acid without using enzymes, we previously selected a DNA aptamer for uric acid with a Kd of 1 μM but the aptamer required multiple Na+ ions for binding. Saturated binding was achieved with around 700 mM Na+ and the binding at the physiological condition was much weaker. In this work, a new selection was performed by alternating Mg2+‐containing buffers with Na+ and Li+. After 13 rounds of selection, a new aptamer sequence named UA‐Mg‐1 was obtained. Isothermal titration calorimetry confirmed aptamer binding in both selection buffers, and the Kd was around 8 μM. The binding of UA‐Mg‐1 to UA required only Mg2+. This is an indicator of successful switching of metal dependency via the salt‐toggled selection method. The UA‐Mg‐1 aptamer was engineered into a fluorescent biosensor based on the strand‐displacement assay with a limit of detection of 0.5 μM uric acid in the selection buffer. Finally, comparison with the previously reported Na+‐dependent aptamer and a xanthine/uric acid riboswitch was also made.

show abstract

Directed Evolution of Aptamer Discovery Technologies

Cited by 38 publications

References 68 publications

Future of Digital Assays to Resolve Clinical Heterogeneity of Single Extracellular Vesicles

Future of Digital Assays to Resolve Clinical Heterogeneity of Single Extracellular Vesicles

Bio-inspired aptamers decorated gold nanoparticles enable visualized detection of malathion

Salt‐Toggled Capture Selection of Uric Acid Binding Aptamers

Contact Info

Product

Resources

About