Direct ultrasensitive electrochemical detection of breast cancer biomarker-miRNA-21 employing an aptasensor based on a microgel nanoparticle composite

Hou, Zhipeng; Zheng, Jun; Zhang, Chengfang; T, Li; Chen, Die; Hu, Liang; Hu, Jun-Rui; Xiong, Bing; Ye, Huarong; Jaffrézic‐Renault, Nicole; Guo, Zhenzhong

doi:10.1016/j.snb.2022.132067

Cited by 25 publications

(9 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…HP3 is opened in the same way as HP2. Subsequently, the freed toehold sequence of HP3 hybridizes with HP1 to form tripedal DNA walker, where the number of miR‐21 complementary bases decreases, thus miR‐21 loses its binding affinity and is therefore released for the next round of reactions [16] . This cyclic process continues autonomously until all HPs are assembled into tripedal DNA walkers equipped with three ssDNA ends that recognize the hairpins on AuNPs.…”

Section: Resultsmentioning

confidence: 99%

Ultrasensitive miRNA Detection by AuNP‐based 3D DNA Walker and Catalytic Hairpin Assembly (CHA) Cascade Amplification for Early Cancer Diagnosis

2023

Chemistry An Asian Journal

View full text Add to dashboard Cite

By combining exquisitely designed hairpins with the catalytic hairpin assembly (CHA) to form tripedal DNA walkers driven by enzyme, we constructed a 3D DNA walker with accordingly complementary hairpins attached on gold nanoparticles (AuNPs) and sensitive fluorescence sensing system for the sensitive detection of target miRNA-21 (miR-21). The presence of miR-21 triggers the CHA among three hairpins (HP1, HP2, and HP3), which lead to the formation of the tripedal DNA walkers. For the walking trajectories, FAMlabeled hairpins (HP4) were attached to the surface of AuNPs, the fluorescence of which was initially quenched due to its close proximity to AuNPs. After the binding/cleaving/moving process of tripedal DNA walkers with HP4 driven by Exonuclease III (Exo III), a number of single-stranded DNAs (ssDNAs) will be released with FAM fluorescence recovered. Benefiting from the DNA walker and CHA cascade amplification, the proposed sensing strategy showed remarkable improvement in sensitivity with the LOD of 42 aM. Owing to the precise design of the system, this method exhibited excellent specificity to distinguish miR-21 from its single-, double-mismatched sequences and non-complementary sequences, showing great versatility and potential for the biological analysis and early disease diagnosis.

show abstract

Section: Resultsmentioning

confidence: 99%

Ultrasensitive miRNA Detection by AuNP‐based 3D DNA Walker and Catalytic Hairpin Assembly (CHA) Cascade Amplification for Early Cancer Diagnosis

2023

Chemistry An Asian Journal

View full text Add to dashboard Cite

show abstract

“…A highly sensitive porous and highly biocompatible microgel nanocomposite based on AuNPs, polyacrylic acid, and N-isopropylacrylamide was used and allowed the covalent immobilization of the amino-modified oligonucleotide capture probe complementary to the target. An optimized DPV procedure was applied to monitor the hybridization of miRNA-21 with the capture probe in serum [ 72 ].…”

Section: Biomarkers Involved In Inflammatory Diseasesmentioning

confidence: 99%

Point-of-care electrochemical testing of biomarkers involved in inflammatory and inflammatory-associated medical conditions

et al. 2022

View full text Add to dashboard Cite

Recent years have shown that the diagnosis and monitoring of biomarkers involved in inflammatory-associated medical conditions such as cancer, neurological disorders, viral infections, or daily physical activities offer real benefits in increasing the quality of medical care and patient life quality. In this context, the use of integrated and portable platforms as point-of-care testing devices for biomedical analysis to enable early disease diagnosis and monitoring, which can be successfully used even at the patient’s bed, is an emergency nowadays. The development of low-cost, miniaturized, and portable, user-friendly devices that provide an answer in a timely manner, such as electrochemical sensors, is relevant for the elaboration of point-of-care testing devices. This review focuses on the recent progress in bioanalysis of both specific biomarkers and inflammatory-associated biomarkers present in several diseases like neoplasia, severe neurological disorders, viral infections, and usual physical activity and provides an overview of the state of the art over the most recent electrochemical (bio)sensors for the detection of inflammation-related biomarkers. Future perspectives of point-of-care testing to improve healthcare management are also discussed. Graphical abstract

show abstract

“…The analytical performance was superior to much reported methods (Table 2). [43][44][45][46][47][48][49][50][51] 3.5. Specificity, stability and reproducibility Two miRNA sequences (miRNA-199a and miRNA-141) and three mismatched base sequences (NC, smRNA, and tmRNA) were selected to study the specificity of the sensor.…”

Section: Performance Analysismentioning

confidence: 99%

Superior performance of a graphdiyne self-powered biosensor with exonuclease III-assisted signal amplification for sensitive detection of microRNAs

Shi

Lin

Qin

et al. 2022

Analyst

View full text Add to dashboard Cite

show abstract

Direct ultrasensitive electrochemical detection of breast cancer biomarker-miRNA-21 employing an aptasensor based on a microgel nanoparticle composite

Cited by 25 publications

References 35 publications

Ultrasensitive miRNA Detection by AuNP‐based 3D DNA Walker and Catalytic Hairpin Assembly (CHA) Cascade Amplification for Early Cancer Diagnosis

Ultrasensitive miRNA Detection by AuNP‐based 3D DNA Walker and Catalytic Hairpin Assembly (CHA) Cascade Amplification for Early Cancer Diagnosis

Point-of-care electrochemical testing of biomarkers involved in inflammatory and inflammatory-associated medical conditions

Superior performance of a graphdiyne self-powered biosensor with exonuclease III-assisted signal amplification for sensitive detection of microRNAs

Contact Info

Product

Resources

About