Direct Tissue Blotting for Detection of Tomato Spotted Wilt Virus inImpatiens

The Comparison of Antibodies Raised Against PLRV with Two Different Approaches - Viral Particles Purification and Recombinant Production of CP

“…The similar result detected by the Indirect ELISA had a greater sensitivity than either DAS-or cocktail-ELISA. The observed high sensitivity of indirect ELISA compared with DAS-ELISA could be due to the binding of more virus antigen on plates that were not percolated with specific antibodies, as suggested by Hsu and Aebig and cited by Hsu and Lawson [48]. Moreover, when evaluating the sensitivity of indirect and DAS-ELISA, their relative ability for accurate quantitation of viral antigens in crude extracts should be considered.…”

Serology Applied to Plant Virology

“…The tissue immunoblotting assay (TIBA) can be used to detect virus antigens in plant tissues such as leaf, stem, bulb, tuber, root and fruit or insect vectors of plant viruses. The tissues are cut with razor blades and pressed on the membrane to transfer the virus particles or protein (Hsu & Lawson, 1991;Makkouk et al, 1993;Navot et al, 1989;Polston et al, 1991;Purcifull et al, 2001). The detection of the virus antigens applied on the membrane is accomplished by protocols similar to those used for indirect ELISA or PTA-ELISA (Figure 3) and for DIBA.…”

Bread wheat x Agrotricum crosses as a source of immunity and resistance to the PAV strain of barley yellow dwarf luteovirus

“…In F 4 a small subsample of 10 wild and 10 wheat-like phenotypes was used for cytogenetic verification. From F 3 to F 5 , the only selection pressure applied was from mechanical threshing, as the Agrotricum-like phenotypes were more difficult to thresh than the wheat-like phenotypes, and therefore more subject to mechanical elimination (Comeau et al, 1993a (Sutula et al, 1986 The TBIA procedure was as previously reported (Hsu et al, 1990;Hsu and Lawson, 1991;Makkouk et al, 1993). Young or old stems were cut by a single-edge razor blade and directly blotted onto a nitrocellulose membrane (no BA85, 0.85 μm, Schleischer and Schuell).…”