Direct tissue analysis using matrix‐assisted laser desorption/ionization mass spectrometry: practical aspects of sample preparation

Schwartz, Sarah; Reyzer, Michelle L.; Caprioli, Richard M.

doi:10.1002/jms.505

Cited by 584 publications

(696 citation statements)

References 16 publications

(33 reference statements)

Supporting

Mentioning

692

Contrasting

Unclassified

Order By: Relevance

“…For stigmatic MALDI experiments, tissues were briefly washed in cold 70% ethanol and dried at room temperature before matrix deposition. 43 Matrix Deposition. ME-SIMS matrix was applied using electrospray deposition as described previously.…”

Section: Methodsmentioning

confidence: 99%

Gold-Enhanced Biomolecular Surface Imaging of Cells and Tissue by SIMS and MALDI Mass Spectrometry

et al. 2005

View full text Add to dashboard Cite

Surface metallization by plasma coating enhances desorption/ionization of membrane components such as lipids and sterols in imaging time-of-flight secondary ion mass spectrometry (TOF-SIMS) of tissues and cells. High-resolution images of cholesterol and other membrane components were obtained for neuroblastoma cells and revealed subcellular details (resolving power 1.5 µm). Alternatively, in matrix-enhanced SIMS, 2,5-dihydroxybenzoic acid electrosprayed on neuroblastoma cells allowed intact molecular ion imaging of phosphatidylcholine and sphingomyelin at the cellular level. Gold deposition on top of matrix-coated rat brain tissue sections strongly enhanced image quality and signal intensity in stigmatic matrixassisted laser desorption/ionization imaging mass spectrometry. High-quality total ion count images were acquired, and the neuropeptide vasopressin was localized in the rat brain tissue section at the hypothalamic area around the third ventricle. Although the mechanism of signal enhancement by gold deposition is under debate, the results we have obtained for cells and tissue sections illustrate the potential of this sample preparation technique for biomolecular surface imaging by mass spectrometry.Unraveling the spatial distribution of cellular membrane components is an important research topic in current molecular cell biology. Understanding the behavior and function of the major constituents of these membranes, i.e., lipids and sterols, has been hampered by methodological limitations, despite their relatively simple structures. Most of the current knowledge on lipid localization has been obtained using fluorescence imaging techniques.

show abstract

Section: Methodsmentioning

confidence: 99%

Gold-Enhanced Biomolecular Surface Imaging of Cells and Tissue by SIMS and MALDI Mass Spectrometry

et al. 2005

View full text Add to dashboard Cite

show abstract

“…Previous studies have highlighted the need to consider sample preparation as a critical component in developing MALDI IMS applications [4,8,39]. However, in spite of the effort to exert control over environmental factors affecting matrix applications, instrument variation during the course of data collection, and inherent cellular heterogeneity within the samples themselves, there is inevitably some contribution of each of these effects to the image.…”

Section: Processing Of Imaging Mass Spectrometry Datamentioning

confidence: 99%

Processing MALDI mass spectra to improve mass spectral direct tissue analysis

Norris¹,

Cornett

Mobley

et al. 2007

International Journal of Mass Spectrometry

Self Cite

190

188

View full text Add to dashboard Cite

Profiling and imaging biological specimens using MALDI mass spectrometry has significant potential to contribute to our understanding and diagnosis of disease. The technique is efficient and high-throughput providing a wealth of data about the biological state of the sample from a very simple and direct experiment. However, in order for these techniques to be put to use for clinical purposes, the approaches used to process and analyze the data must improve. This study examines some of the existing tools to baseline subtract, normalize, align, and remove spectral noise for MALDI data, comparing the advantages of each. A preferred workflow is presented that can be easily implemented for data in ASCII format. The advantages of using such an approach are discussed for both molecular profiling and imaging mass spectrometry.

show abstract

“…After washing, the tissue sections were allowed to dry for 30 min after which the matrix was°applied° [41]°using°a°TLC°sprayer°[42,°43]°(30 mg/mL of 2,5-dihydroxybenzoic acid in 50:50 ethanol/ water and 0.1% trifluoroacetic acid). The sample was then covered with a 3 nm layer of gold using a plasma sputter coater (Quorum Technologies, Newhaven, United Kingdom).…”

Section: Tissue Sectionsmentioning

confidence: 99%

Imaging of peptides in the rat brain using MALDI-FTICR mass spectrometry

Taban

Altelaar

Burgt

et al. 2007

J. Am. Soc. Mass Spectrom.

148

136

View full text Add to dashboard Cite

Analytical methods are pursued to measure the identity and location of biomolecules down to the subcellular ( m) level. Available mass spectrometric imaging methods either compromise localization accuracy or identification accuracy in their analysis of surface biomolecules. In this study, imaging FTICR-MS is applied for the spatially resolved mass analysis of rat brain tissue with the aim to optimize protein identification by the high mass accuracy and online MS/MS capabilities of the technique. Mass accuracies up to 6 ppm were obtained in the direct MALDI-analysis of the tissue together with a spatial resolution of 200 m. The spatial distributions of biomolecules differing in mass by less than 0.1 Da could be resolved, and are shown to differ significantly. Online MS/MS analysis of selected ions was demonstrated. A comparison of the FTICR-MS imaging results with stigmatic TOF imaging on the same sample is presented. To reduce the extended measuring times involved, it is recommended to restrict the FTICR-MS analyses to areas of interest as can be preselected by other, faster imaging methods. (J Am Soc Mass Spectrom 2007, 18, 145-151)

show abstract

Direct tissue analysis using matrix‐assisted laser desorption/ionization mass spectrometry: practical aspects of sample preparation

Cited by 584 publications

References 16 publications

Gold-Enhanced Biomolecular Surface Imaging of Cells and Tissue by SIMS and MALDI Mass Spectrometry

Gold-Enhanced Biomolecular Surface Imaging of Cells and Tissue by SIMS and MALDI Mass Spectrometry

Processing MALDI mass spectra to improve mass spectral direct tissue analysis

Imaging of peptides in the rat brain using MALDI-FTICR mass spectrometry

Contact Info

Product

Resources

About