Direct somatic embryogenesis on leaf explants of Oncidium Gower Ramsey and subsequent plant regeneration

Chen, Jen‐Tsung; Chang, Ching-Jin; Chang, Wei‐Chin

doi:10.1007/s002990050724

Cited by 96 publications

(85 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The formed PLBs shot primordia after 18-24 weeks of culture ( Figure 3G), and shoots and roots regenerated after 32 weeks of culture ( Figure 3H). Similar results have been observed in Oncidium orchids [10], where the PLBs were directly differentiated from the leaf epidermal tissue.…”

Section: The Effect Of Plant Growth Regulator In Plb Inductionsupporting

confidence: 88%

Regeneration of Plantlets Through PLB (Protocorm-Like Body) Formation in Phalaenopsis ‘Join Angle X Sogo Musadian’

Meilasari¹,

Iriawati²

2016

j.math.fund.sci.

View full text Add to dashboard Cite

Abstract. Selection and regeneration of specific hybrids of Phalaenopsis typically takes a long time since this plant usually reproduces through seeds. This study was conducted to examine the best medium and explants for regeneration of Phalaenopsis 'Join Angle x Sogo Musadian'. In vitro and in vivo roots and leaves were used as explants, which were cultured in half-strength Murashige and Skoog medium supplemented with various combinations of plant growth regulators (TDZ, 2,4-D, NAA, BAP and IAA). The results showed that the in vitro roots produced the highest number of PLBs (49.3 PLBs) when they were cultured in medium containing 0.5 ppm NAA, 5 ppm BAP, and 0.5 ppm IAA. The in vitro leaf explants also regenerated PLBs, however, only two PLBs developed, i.e. when they were cultured in medium containing 1 ppm TDZ and 2 ppm 2,4-D. PLB germination into plantlets was performed by culturing each PLB on MS medium without plant growth regulators. Based on the results of the present study, it can be concluded that in vitro roots and half-strength MS medium supplemented with 0.5 ppm NAA, 5 ppm BAP, and 0.5 ppm IAA are the best explant and best medium respectively for plant regeneration through PLBs.

show abstract

Section: The Effect Of Plant Growth Regulator In Plb Inductionsupporting

confidence: 88%

Regeneration of Plantlets Through PLB (Protocorm-Like Body) Formation in Phalaenopsis ‘Join Angle X Sogo Musadian’

Meilasari¹,

Iriawati²

2016

j.math.fund.sci.

View full text Add to dashboard Cite

show abstract

“…Studies were being intensively made to develop a protocol for somatic embryogenesis. The process can be induced in tissue cultures of orchids either directly from the epidermal cells of explants [1][2][3] or indirectly via intervening callus [4]. In this study, somatic embryogenesis was induced indirectly through callus culture.…”

Section: Resultsmentioning

confidence: 99%

“…However, there is no report on plant regeneration through somatic embryogenesis in C. cristata. The process has been induced in tissue cultures of orchids either directly from the epidermal cells of explants [1][2][3] or indirectly via intervening callus [4].…”

Section: Introductionmentioning

confidence: 99%

Plant Regeneration Through Indirect Somatic Embryogenesis in Coelogyne Cristata Orchid

Naing¹,

Chung²,

Lim³

2011

AJPS

View full text Add to dashboard Cite

show abstract

“…Os resultados obtidos foram similares aos relatados por Chen et al (1999) com Oncidium 'Gower Ramsey'. Segmentos foliares produziram aglomerados de massas nodulares quando cultivados em meio de cultura MS/2 suplementado com 2,4-D e TDZ.…”

Section: Resultados E Discussão Efeito Dos Reguladores De Crescimentounclassified

“…Apesar da formulação salina de Knudson (1946) ser a mais empregada para o cultivo in vitro de orquídeas, bons resultados têm sido obtidos em meio de cultura MS (MURASHIGE & SKOOG, 1962) por vários autores (CHEN et al, 1999;CHEN & CHANG, 2002FARIA et al, 2004). Porém, torna-se necessário determinar as condições ótimas requeridas de nutrientes, pH do meio de cultura, reguladores e carboidratos para cada variedade (ARAÚJO et al, 2005).…”

Section: Introductionunclassified

Conversão in vitro de raízes e folhas de Miltonia flavescens Lindl. em protocormos e regeneração de plantas

et al. 2009

View full text Add to dashboard Cite

RESUMOObjetivou-se, no presente trabalho, avaliar o efeito do 2,4-D e do BAP na formação de protocormos a partir de regiões meristemáticas da raiz e segmentos foliares de Miltonia flavescens Lindl., bem como testar o efeito de concentrações de sacarose e níveis de pH no crescimento in vitro dos protocormos. Ápices radiculares e segmentos de folhas de plantas germinadas in vitro foram inoculados em meio de cultura MS/2 suplementado com 2,4-D e BAP onde permaneceram por 210 dias no escuro, sendo transferidos para a luz e inoculados em meio de cultura isento de reguladores, avaliando-se a porcentagem de explantes com protocormos e o número médio de protocormos regenerados por explante. A associação de 3 mg L -1 de 2,4-D e 1 ou 3 mg L -1 de BAP proporcionou os melhores resultados quanto à formação de protocormos. Esses foram cultivados em meio de cultura MS/2 onde testou-se o efeito da sacarose (0, 15, 30, 45 e 60 g L -1 ) e do pH (5.0, 5.8 e 6.6) sobre o seu crescimento. As concentrações de 15 e 30 g L -1 de sacarose independentes do pH, mostraram-se mais eficientes para o cultivo in vitro da espécie proporcionando a formação de um maior número e comprimento de raízes, altura de parte aérea e peso da matéria fresca. O pH só apresentou-se significativo quando em combinação com 15 g L -1 de sacarose para as variáveis comprimento da maior raiz e peso da matéria fresca.Termos para indexação: Orchidaceae, reguladores de crescimento, sacarose, pH, Miltonia flavescens. ABSTRACTThe aim of the present work was to evaluate the effect of 2,4-D and BAP on the protocorm formation from meristematic areas of the root and leaf segments of Miltonia flavescens Lindl., as well as to test the effect of sucrose concentrations and pH levels on the in vitro growth of protocorms. Root apexes and leaf segments of in vitro-germinated plants were cultured on MS/2 culture medium supplemented with 2,4-D and BAP and kept in the dark for 210 days. Explants were then transferred to light and cultured on culture medium lacking growth regulators. Percentage of explants with protocorms and average number of protocorms regenerated per explant were evaluated. The combination of 3 mg L -1 2,4-D and 1 or 3 mg L -1 BAP provided the best results for protocorm formation. Protocorms were cultured on MS/2 culture medium to test the effect of sucrose (0, 15, 30, 45, and 60 g L -1 ) and pH (5.0, 5.8, and 6.6) on their growth. The concentrations of 15 and 30 g L -1 sucrose, independently of the pH, were more efficient for the in vitro culture of this species, promoting an increase in the number and length of the roots, plant height, and fresh weight. The pH was significant only in combination with 15 g L -1 sucrose for the variables length of the largest root and fresh weight.

show abstract

Direct somatic embryogenesis on leaf explants of Oncidium Gower Ramsey and subsequent plant regeneration

Cited by 96 publications

References 8 publications

Regeneration of Plantlets Through PLB (Protocorm-Like Body) Formation in Phalaenopsis ‘Join Angle X Sogo Musadian’

Regeneration of Plantlets Through PLB (Protocorm-Like Body) Formation in Phalaenopsis ‘Join Angle X Sogo Musadian’

Plant Regeneration Through Indirect Somatic Embryogenesis in Coelogyne Cristata Orchid

Conversão in vitro de raízes e folhas de Miltonia flavescens Lindl. em protocormos e regeneração de plantas

Contact Info

Product

Resources

About