Direct shoot regeneration from leaf explants of Digitalis lamarckii, an endemic medicinal species

Verma, Sandeep Kumar; Yücesan, Bahtiyar Buhara; Cingoz, Gunce Sahin; Gürel, Songül; Gürel, Ekrem

doi:10.3906/bot-1101-42

Cited by 9 publications

(4 citation statements)

References 40 publications

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“…The results of this study agree with the results of Paul et al (2012), showing the superiority of BAP supplemented with low concentrations of NAA in the MS medium, the results of Adsul et al (2019) study on Ceropegia mohanramii, and the results of Verma et al (2011) study on direct organ regeneration from explants of Digitalis lamarckii Ivan.…”

Section: The Effect Of the Benzylaminopurine (Bap)-with-auxin Interac...supporting

confidence: 88%

Effects of basic media and plant growth regulators on Direct-somatic embryogenesis induction in vegetative strains obtained from the tissue culture of digitalis purpurea

2023

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This study aimed to investigate the effects of certain nutrient medium types enhanced with plant-growth regulators on the direct inductionof somatic embryogenesis in vegetative strains produced and propagated from a tissue culture of Digitalis purpurea. The results showthat MS medium was superior to other tested nutrient media-B5, LS, and 5C01—in terms of the days required to initiate direct somaticembryogenesis induction and induction rate. In contrast, the LS medium resulted in the highest number of embryos. Moreover, 2 mg/LBA (benzyl adenine) was superior to similar concentrations of BAP (benzyl aminopurine) and Kin (kinetin) in the MS medium, whereasBAP performed better in LS and B5. On the other hand, auxin 2.4D (dichlorophenoxy acetic acid) inhibited somatic embryogenesis, as didNAA (naphthalene acetic acid) and IAA (indole acetic acid) when added individually. Regarding the type of explant, the stems showedthe induction rates and numbers of direct-somatic embryos produced in all the basic media.Keywords: Digitalis purpurea; Vegetative strains; Direct-somatic embryogenesis; Explant; Basic media

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Section: The Effect Of the Benzylaminopurine (Bap)-with-auxin Interac...supporting

confidence: 88%

Effects of basic media and plant growth regulators on Direct-somatic embryogenesis induction in vegetative strains obtained from the tissue culture of digitalis purpurea

2023

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“…Similar concentrations have been recommended for many other medicinal plants, such as Phalaenopsis cv. [38], Digitalis lamarckii, an endemic medicinal species [39] and Salvia sclarea L. [40]. Increasing concentration of auxins (up to 1.50 mg/L) decreased root formation frequency.…”

Section: Discussionmentioning

confidence: 99%

In vitro Rapid Regeneration of Bitter Melon (Momordica charantia L.) Using Direct Organogenesis in Kenya

Naitchede¹,

Runo²,

Nyende³

2022

ujar

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“…There were many reports for effects of the growth regulators on shoot multiplication in dendrobium micropropagation [20,21,22] . In this study, beside studying the effects of growth regulators on shoot multiplication and biomass, the authors also evaluated the chemical composition in DO in vitro biomass to make a judgment in the extraction of DO biomass for medicinal and food purposes.The in vitro biomass productions of Dendrobium officinale were analyzed to determine the main chemical components and the results are shown in Table 3.…”

Section: Chemical Parameter Of Biomass Of In Vitro Dendrobium Officinalementioning

confidence: 99%

Effects of culture medium composition on shoot multiplication and biomass production by in vitro culture of Dendrobium officinale (Kimura et Migo)

Hong Van-Nguyen,

Ung Quang-Le,

Tho Huu- Nguyen

2023

Magna Sci. Adv. Res. Rev.

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Dendrobium officinale (Kimura et Migo) is one of the most valuable and rare medicinal orchids exhausted in nature. The production of artificial plant biomass in this orchid is really necessary to prevent this risk. This study aimed to evaluate the effect of growth regulators (BAP, NAA, and GA3) on in vitro biomass production of Dendrobium officinale. Experiments were arranged in a completely randomized design (CRD) with three replicates. The results showed that MS medium supplemented with sucrose 30 gL-1, agar 6 gL-1, BAP gL-1, and NAA 0,5 gL-1 was the most suitable medium for in vitro biomass production. In vitro biomass of orchids cultured on the medium contained chemical components commonly found in wild dendrobium such as: Ca, Mg, protein, polysaccharides, and alkaloids. The success of this study is the basis for in vitro biomass production in order to supply materials for the medicine and food industries.

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Direct shoot regeneration from leaf explants of Digitalis lamarckii, an endemic medicinal species

Cited by 9 publications

References 40 publications

Effects of basic media and plant growth regulators on Direct-somatic embryogenesis induction in vegetative strains obtained from the tissue culture of digitalis purpurea

Effects of basic media and plant growth regulators on Direct-somatic embryogenesis induction in vegetative strains obtained from the tissue culture of digitalis purpurea

In vitro Rapid Regeneration of Bitter Melon (Momordica charantia L.) Using Direct Organogenesis in Kenya

Effects of culture medium composition on shoot multiplication and biomass production by in vitro culture of Dendrobium officinale (Kimura et Migo)

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