Direct shoot organogenesis from shoot tip explants of a highly medicinal valued tree Pterocarpus marsupium Roxb.

Ahmad, Anees; Anis, Mohammad; Khanam, Mehrun Nisha; Alatar, Abdulrahman A.

doi:10.1007/s11627-020-10115-7

Cited by 11 publications

(11 citation statements)

References 42 publications

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“…Our results were similar to reports of other woody trees. A combination of a higher cytokinin-to-auxin ratio to the medium was beneficial to induce multiple shoots (Zhu et al 2019;Zhu et al 2010;Tippani et al 2013;Ahmad et al 2020). In our study, compared with CKs alone in the medium, exogenous application of high concentration CKs (KT and TDZ) along with a low dose of auxins did not significantly improve the adventitious bud induction parameters of Korean pine.…”

Section: De Novo Shoot Organogenesis From Korean Pine Sprouted Seeds Explantscontrasting

confidence: 52%

“…In addition, when treated with IBA singly, a small amount of callus appeared and resulted in poor rooting (very few or no roots). Similarly, the recent study of in vitro rooting from Pterocarpus marsupium also found that beyond the optimum dose of IBA induced undesirable callus at the basal end of microshoot, and no rooting was observed (Ahmad et al 2020). Otherwise, for applied PGRs in vitro rooting, there are some different results, supplementation with 1.2 uM NAA in the medium was effective for rooting induction in P. massoniana (Wang and Yao 2019b), most of the clones from Pinus densiflora rooted on medium supplemented with 0.2 mg • L −1 NAA (Zhu et al 2019).…”

Section: Rooting and Acclimatization Of Pinus Koraiensis Microshootsmentioning

confidence: 76%

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Direct in vitro organogenesis from sprouted seeds of a highly economical and ecological valued tree, Korean pine

Liang

Bai

et al. 2021

Plant Cell Tiss Organ Cult

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The de novo organogenesis system for Korean pine of this economically and ecologically coniferous species was successfully established using sprouted seeds as the original explants. After 30 days of incubation, 92.67% of explants produced direct shoots on Gupta and Durzan(DCR) medium containing 2 mg • L −1 kinetin (KT) in combination 0.5 mg • L −1 thidiazuron (TDZ) with a maximum of about 15 shoots per explant respectively. We also confirmed the organogenic regeneration pattern by scanning electron microscopy (SEM) observation. For shoot elongation and growth after 60 days of culture, we obtained the highest mean length of 34.99 mm from DCR basal media supplemented with 6-benzyladenine (6-BA; 0.2 mg • L −1 ), 1-Naphthaleneacetic acid (NAA; 0.1 mg • L −1 ), and activated charcoal (AC; 1 g • L −1 ). The highest rooting percentages of 20.74-21.48% were observed within two months in the 1/2 DCR medium (major elements halved) enriched with 0.05 mg•L −1 NAA and 0.5 or 1 mg • L −1 indole-3-butyric acid (IBA).Rooted plants showed a survival rate of 90.28% in perlite: peat: vermiculite = 1:1:1 after acclimatization. This protocol is a successful and efficient biotechnological approach to the micropropagation of Korean pine, and these data will be helpful to the clonal propagation and conservation of Korean pine.

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Section: De Novo Shoot Organogenesis From Korean Pine Sprouted Seeds Explantscontrasting

confidence: 52%

Section: Rooting and Acclimatization Of Pinus Koraiensis Microshootsmentioning

confidence: 76%

Direct in vitro organogenesis from sprouted seeds of a highly economical and ecological valued tree, Korean pine

Liang

Bai

et al. 2021

Plant Cell Tiss Organ Cult

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“…Shoot tip culture is the most appropriate method for obtaining both virus-free and true-to-type clones. Up to now, only a single report has been published on successful in vitro regeneration of P. marsupium through shoot tip explants [ 149 ]. The highest shoot bud differentiation was achieved in this investigation at 7.0 µM m T plus 1.0 µM NAA, whereas Das and Chatterjee [ 96 ] attempted to regenerate multiple shoots from the shoot tip of P. marsupium .…”

Section: Propagation Of Pterocarpus Marsupiummentioning

confidence: 99%

“…Cumulatively, the results of these studies are clear evidence that intact seedling cultures are greatly influenced by the formulation of the growth regulator and that excision of the explant is not necessary for in vitro shoot multiplication. In case of P. marsupium , Ahmad, Anis, Khanam and Alatar [ 149 ] reported only direct shoot multiplication from the intact seedling method. The optimum culture, containing 0.50 µM GA 3 , induced the maximum seed germination response along with better shoot growth when compared to cultures with TDZ.…”

Section: Propagation Of Pterocarpus Marsupiummentioning

confidence: 99%

“…However, it was found that the two hormones combined together at an optimal level (TDZ at 0.50 µM and GA 3 at 0.50 µM) resulted in the most effective treatment for germination response, shoot multiplication, and subsequent shoot elongation per explant. The authors [ 149 ] hypothesized that the improvement in protocol performance was due to the interaction of the two hormones, with successful regulation both in terms of biosynthesis and signal transduction.…”

Section: Propagation Of Pterocarpus Marsupiummentioning

confidence: 99%

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Biotechnological Advances in Pharmacognosy and In Vitro Manipulation of Pterocarpus marsupium Roxb.

Ahmad

Anis

et al. 2022

Plants

Self Cite

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Trees are vital resources for economic, environmental, and industrial growth, supporting human life directly or indirectly through a wide variety of therapeutic compounds, commodities, and ecological services. Pterocarpus marsupium Roxb. (Fabaceae) is one of the most valuable multipurpose forest trees in India and Sri Lanka, as it is cultivated for quality wood as well as pharmaceutically bioactive compounds, especially from the stem bark and heartwood. However, propagation of the tree in natural conditions is difficult due to the low percentage of seed germination coupled with overexploitation of this species for its excellent multipurpose properties. This overexploitation has ultimately led to the inclusion of P. marsupium on the list of endangered plant species. However, recent developments in plant biotechnology may offer a solution to the overuse of such valuable species if such advances are accompanied by technology transfer in the developing world. Specifically, techniques in micropropagation, genetic manipulation, DNA barcoding, drug extraction, delivery, and targeting as well as standardization, are of substantial concern. To date, there are no comprehensive and detailed reviews of P. marsupium in terms of biotechnological research developments, specifically pharmacognosy, pharmacology, tissue culture, authentication of genuine species, and basic gene transfer studies. Thus, the present review attempts to present a comprehensive overview of the biotechnological studies centered on this species and some of the recent novel approaches for its genetic improvement.

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Polyamines Mediated In Vitro Morphogenesis in Cotyledonary Node Explants of Mucuna pruriens (L.) DC.: A Natural Source of L-Dopa

Ansari

Ahmad

et al. 2023

J Plant Growth Regul

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Direct shoot organogenesis from shoot tip explants of a highly medicinal valued tree Pterocarpus marsupium Roxb.

Cited by 11 publications

References 42 publications

Direct in vitro organogenesis from sprouted seeds of a highly economical and ecological valued tree, Korean pine

Direct in vitro organogenesis from sprouted seeds of a highly economical and ecological valued tree, Korean pine

Biotechnological Advances in Pharmacognosy and In Vitro Manipulation of Pterocarpus marsupium Roxb.

Polyamines Mediated In Vitro Morphogenesis in Cotyledonary Node Explants of Mucuna pruriens (L.) DC.: A Natural Source of L-Dopa

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