Direct SARS-CoV-2 Nucleic Acid Detection by Y-Shaped DNA Dual-Probe Transistor Assay

Kong, Dewen; Wang, Xuejun; Gu, Cheng; Guo, Mingquan; Wang, Yao; Ai, Zhaolin; Zhang, Shen; Chen, Yiheng; Li, Wentao; Wu, Yungen; Dai, Changhao; Guo, Qianying; Qu, Di; Zhu, Zhaoqin; Xie, Youhua; Liu, Yunqi; Wei, Dacheng

doi:10.1021/jacs.1c06325

Cited by 91 publications

(93 citation statements)

References 72 publications

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“…In biological buffer solutions, the charged protein molecules would be surrounded by oppositely charged ions due to electrostatic interaction, thus decreasing the effective charge of proteins. [ 28–41 ] This effect is known as Debye screening, and due to the Debye screening effect, the effective electrical field of FET biosensors could only be controlled within a distance called the Debye length (λ D ): [ 42,43 ]

{\lambda _{\rm{D}}} = \sqrt {\frac{{\varepsilon {k_{\rm{B}}}T}}{{2{N_{\rm{A}}}{q^2}I}}}

where ε is the dielectric constant of electrolyte. k B , T , and N A represent the Boltzmann's constant, temperature, and Avogadro's number, respectively.…”

Section: Resultsmentioning

confidence: 99%

Integrated Urinalysis Devices Based on Interface‐Engineered Field‐Effect Transistor Biosensors Incorporated With Electronic Circuits

et al. 2022

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BC diagnosis relies on the insertion of an optical endoscope into the bladder cavity through urethra to image the suspected lesions. [7,8] This process is highly invasive and would cause urethra and bladder injury, resulting in hematuria and even urinary bacterial infection within a few days after examinations. [9] Critically, cystoscopy diagnosis is plagued with the inherent bladder tumor heterogeneity, and therefore, limits the accuracy of early BC diagnosis. [10] Recently, noninvasive liquid biopsy emerges as an alternative to address the bottleneck of spatiotemporal tumor heterogeneity and to obtain disease-relevant molecular information for clinical cancer diagnosis and cancer status monitoring. [11][12][13][14][15][16][17][18][19][20] Bladder is a urine storage organ that has been recognized as the metabolic microenvironment for bladder tumor cells, and its carcinogenesis and progression could make a pivotal impact on urine. [21] In this regard, the development of a urine biopsy provides a powerful strategy toward noninvasive early diagnosis and prognosis of BC. Clinically, urinalysis has been routinely utilized to detect abnormal metabolic biomarkers in urine for the assessment of health status and preliminary screening of diseases. [22][23][24][25] However, the physiologically relevant biomarkers detected by routine urinalysis are generally limited to high concentration targets over the micromolar level. On the other hand, the concentration Urinalysis is attractive in non-invasive early diagnosis of bladder cancer compared with clinical gold standard cystoscopy. However, the trace bladder tumor biomarkers in urine and the particularly complex urine environment pose significant challenges for urinalysis. Here, a clinically adoptable urinalysis device that integrates molecular-specificity indium gallium zinc oxide field-effect transistor (IGZO FET) biosensor arrays, a device control panel, and an internet terminal for directly analyzing five bladder-tumor-associated proteins in clinical urine samples, is reported for bladder cancer diagnosis and classification. The IGZO FET biosensors with engineered sensing interfaces provide high sensitivity and selectivity for identification of trace proteins in the complex urine environment. Integrating with a machine-learning algorithm, this device can identify bladder cancer with an accuracy of 95.0% in a cohort of 197 patients and 75 non-bladder cancer individuals, distinguishing cancer stages with an overall accuracy of 90.0% and assessing bladder cancer recurrence after surgical treatment. The non-invasive urinalysis device defines a robust technology for remote healthcare and personalized medicine.

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{\lambda _{\rm{D}}} = \sqrt {\frac{{\varepsilon {k_{\rm{B}}}T}}{{2{N_{\rm{A}}}{q^2}I}}}

where ε is the dielectric constant of electrolyte. k B , T , and N A represent the Boltzmann's constant, temperature, and Avogadro's number, respectively.…”

Section: Resultsmentioning

confidence: 99%

Integrated Urinalysis Devices Based on Interface‐Engineered Field‐Effect Transistor Biosensors Incorporated With Electronic Circuits

et al. 2022

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“…FET can also assist genosensor in recognition of nucleic acids. Kong et al 159 constructed a graphene-based FET containing y-shaped DNA dual probes for highly sensitive recognition of ORF1ab and N genes of SARS-CoV-2 nucleic acids. In addition to conventional electrochemical signals, photoelectrochemical signals can also be used for detection.…”

Section: Keyword Analysis and Evolution Of The Fieldmentioning

confidence: 99%

The role of electrochemical biosensors in SARS-CoV-2 detection: a bibliometrics-based analysis and review

Mao

Yin

et al. 2022

RSC Adv.

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“…Therefore, for the accurate diagnosis of COVID-19 infection, NA testing is the most reliable method. Considering these issues and limitations of the RT-PCR method, electrochemical biosensors for screening SARS-CoV-2 NA were also developed to improve the clinical efficacy and reliability of COVID-19 diagnosis [104][105][106][107]. Kong et al constructed a NA assay using a Y-shaped dual DNA probe-modified graphene FET that could simultaneously target ORF1ab and N genes of SARS-CoV-2 NA (Figure 8a) [105].…”

Section: Electrochemical Nucleic Acid-based Detection Of Sars-cov-2 V...mentioning

confidence: 99%

Progress in Electrochemical Biosensing of SARS-CoV-2 Virus for COVID-19 Management

Rahman

2022

Chemosensors

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Rapid and early diagnosis of lethal coronavirus disease-19 (COVID-19) caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an important issue considering global human health, economy, education, and other activities. The advancement of understanding of the chemistry/biochemistry and the structure of the SARS-CoV-2 virus has led to the development of low-cost, efficient, and reliable methods for COVID-19 diagnosis over “gold standard” real-time reverse transcription-polymerase chain reaction (RT-PCR) due to its several limitations. This led to the development of electrochemical sensors/biosensors for rapid, fast, and low-cost detection of the SARS-CoV-2 virus from the patient’s biological fluids by detecting the components of the virus, including structural proteins (antigens), nucleic acid, and antibodies created after COVID-19 infection. This review comprehensively summarizes the state-of-the-art research progress of electrochemical biosensors for COVID-19 diagnosis. They include the detection of spike protein, nucleocapsid protein, whole virus, nucleic acid, and antibodies. The review also outlines the structure of the SARS-CoV-2 virus, different detection methods, and design strategies of electrochemical SARS-CoV-2 biosensors by highlighting the current challenges and future perspectives.

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Direct SARS-CoV-2 Nucleic Acid Detection by Y-Shaped DNA Dual-Probe Transistor Assay

Cited by 91 publications

References 72 publications

Integrated Urinalysis Devices Based on Interface‐Engineered Field‐Effect Transistor Biosensors Incorporated With Electronic Circuits

Integrated Urinalysis Devices Based on Interface‐Engineered Field‐Effect Transistor Biosensors Incorporated With Electronic Circuits

The role of electrochemical biosensors in SARS-CoV-2 detection: a bibliometrics-based analysis and review

Progress in Electrochemical Biosensing of SARS-CoV-2 Virus for COVID-19 Management

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