Direct recovery of cyclodextringlycosyltransferase from Bacillus cereus using aqueous two-phase flotation

Lin, Yu; Show, Pau Loke; Yap, Yee Jiun; Tan, Chin Ping; Ng, Eng-Poh; Ariff, Arbakariya; Annuar, Mohamad Suffian Mohamad; Ling, Tau Chuan

doi:10.1016/j.jbiosc.2015.04.013

Cited by 26 publications

(8 citation statements)

References 19 publications

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“…A similar approach utilizing polymer-based LBS was employed for the recovery of lignin peroxidase from Amauroderma rugosum (Blume and T. Nees) [33]. However, this experiment used a lower molecular weight (PEG 600) for a high purification of lignin peroxidase.…”

Section: Polymer-based Lbsmentioning

confidence: 99%

Liquid Biphasic System: A Recent Bioseparation Technology

et al. 2020

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A well-known bioseparation technique namely liquid biphasic system (LBS) has attracted many researchers’ interest for being an alternative bioseparation technology for various kinds of biomolecules. The present review begins with an in-depth discussion on the fundamental principle of LBS and this is followed by the discussion on further development of various phase-forming components in LBS. Additionally, the implementation of various advance technologies to the LBS that is beneficial towards the efficiency of LBS for the extraction, separation, and purification of biomolecules was discussed. The key parameters affecting the LBS were presented and evaluated. Moreover, future prospect and challenges were highlighted to be a useful guide for future development of LBS. The efforts presented in this review will provide an insight for future researches in liquid-liquid separation techniques.

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Section: Polymer-based Lbsmentioning

confidence: 99%

Liquid Biphasic System: A Recent Bioseparation Technology

et al. 2020

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“…The pH value of an LBF system affects the separation outcome by altering the surface properties of the target protein, including the surface net charge, molecular shape, surface hydrophobicity, and the presence of specific binding sites [24]. A simple example is the case of a biomolecule with both polar and non-polar groups that experiences changes in its net charge and surface properties with changing pH values [25].…”

Section: Influence Of Ph On the Recovery Yield And Separation Efficiencymentioning

confidence: 99%

Application of a Liquid Biphasic Flotation (LBF) System for Protein Extraction from Persiscaria Tenulla Leaf

et al. 2020

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Persiscaria tenulla, commonly known as Polygonum, is a plant belonging to the family Polygonaceae, which originated from and is widely found in Southeast Asia countries, such as Indonesia, Malaysia, Thailand, and Vietnam. The leaf of the plant is believed to have active ingredients that are responsible for therapeutic effects. In order to take full advantage of a natural medicinal plant for the application in the pharmaceutical and food industries, extraction and separation techniques are essential. In this study, an emerging and rapid extraction approach known as liquid biphasic flotation (LBF) is proposed for the extraction of protein from Persiscaria tenulla leaves. The scope of this study is to establish an efficient, environmentally friendly, and cost-effective technology for the extraction of protein from therapeutic leaves. Based on the ideal conditions of the small LBF system, a 98.36% protein recovery yield and a 79.12% separation efficiency were achieved. The upscaling study of this system exhibited the reliability of this technology for large-scale applications with a protein recovery yield of 99.44% and a separation efficiency of 93.28%. This technology demonstrated a simple approach with an effective protein recovery yield and separation that can be applied for the extraction of bioactive compounds from various medicinal-value plants.

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“…The product degradation and inhibition which normally happen during the conventional product recovery can be eliminated by rapid removal of the product using extractive bioconversion in ATPS (Ng et al 2013;Daugulis 1988;Freeman et al 1993). This method has been demonstrated by Ng et al (2013) in investigating the synthesis and recovery of cyclodextrins (CDs) from Bacillus cereus cyclodextrin glycosyltransferase (CGTase, EC 2.4.1.19) (Lin YK et al 2015). An extractive bioconversion in ATPS consisted of 7.7 % (w/w) PEG 20,000 and 10.3 % (w/w) dextran T500 with volume ratio (VR) of 4.0 were selected as the optimum recovery system of CDs.…”

Section: Application Of Polymer/polymer Atpsmentioning

confidence: 99%

Current applications of different type of aqueous two-phase systems

Yau

Ooi

et al. 2015

Bioresour. Bioprocess.

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In recent year, aqueous two-phase system (ATPS) has become a proven tool used in separation and purification technology. The application of ATPSs in clarification, partitioning and partial purification of biomolecules and bioproducts had showed the rapid development. This method is able to give high recovery yield and high purity in a single step. The ATPS shows characteristics of high selectivity and is easily to scale up. Therefore, ATPS offers an attractive alternative that meets the requirements of the high demand in industry processes and it is also beneficial in terms of economic and environmental protection. In the past, a lot of works and researches have been done in order to develop feasible separation processes using different types of ATPSs and their applications in numerous product separations. This paper aims to review on the recent literature works in the development of different type of ATPSs and their applications in novel separations and purifications of biomaterials.

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Direct recovery of cyclodextringlycosyltransferase from Bacillus cereus using aqueous two-phase flotation

Cited by 26 publications

References 19 publications

Liquid Biphasic System: A Recent Bioseparation Technology

Liquid Biphasic System: A Recent Bioseparation Technology

Application of a Liquid Biphasic Flotation (LBF) System for Protein Extraction from Persiscaria Tenulla Leaf

Current applications of different type of aqueous two-phase systems

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