Current applications of different type of aqueous two-phase systems

Yau, Yee Koon; Ooi, Chien Wei; Ng, Eng-Poh; Lan, John Chi-Wei; Ling, Tau Chuan; Show, Pau Loke

doi:10.1186/s40643-015-0078-0

Cited by 91 publications

(46 citation statements)

References 98 publications

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“…28 To further reduce costs, the recycling of components can be considered. Several studies using ATPS-based technologies have developed this approach 14,29,30 concluding with an economic analysis that demonstrates that the use of additional operations to recover ATPS components does not increase the cost of the final product. 19…”

Section: Deterministic Analysismentioning

confidence: 99%

Economic analysis of pilot‐scale production of B‐phycoerythrin

Torres‐Acosta

Ruiz‐Ruiz

Aguilar‐Yáñez

et al. 2016

Biotechnology Progress

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β-Phycoerythrin is a color protein with several applications, from food coloring to molecular labeling. Depending on the application, different purity is required, affecting production cost and price. Different production and purification strategies for B-phycoerythrin have been developed, the most studied are based on the production using Porphyridium cruentum and purified using chromatographic techniques or aqueous two-phase systems. The use of the latter can result in a less expensive and intensive recovery of the protein, but there is lack of a proper economic analysis to study the effect of using aqueous two-phase systems in a scaled-up process. This study analyzed the production of B-Phycoerythrin using real data obtained during the scale-up of a bioprocess using specialized software (BioSolve, Biopharm Services, UK). First, a sensitivity analysis was performed to identify critical parameters for the production cost, then a Monte Carlo analysis to emulate real processes by adding uncertainty to the identified parameters. Next, the bioprocess was analyzed to determine its financial attractiveness and possible optimization strategies were tested and discussed. Results show that aqueous two-phase systems retain their advantages of low cost and intensive recovery (54.56%); the costs of production per gram calculated (before titer optimization: US$15,709 and after optimization: US$2,374) allowed to obtain profit (in the range of US$millions in a 10-year period) for a potential company taking this production method by comparing the production cost against commercial prices. The bioprocess analyzed is a promising and profitable method for the generation of a highly purified B-phycoerythrin. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:1472-1479, 2016.

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Section: Deterministic Analysismentioning

confidence: 99%

Economic analysis of pilot‐scale production of B‐phycoerythrin

Torres‐Acosta

Ruiz‐Ruiz

Aguilar‐Yáñez

et al. 2016

Biotechnology Progress

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“…It is suggested to use ATPS processes in place of other conventional chromatographic operations to reduce steps of downstream processing [2]. All the processes of extraction and purification of biological products by ATPS occur in single or two steps.…”

Section: Introductionmentioning

confidence: 99%

“…All the processes of extraction and purification of biological products by ATPS occur in single or two steps. It is suggested to use ATPS processes in place of other conventional chromatographic operations to reduce steps of downstream processing [2]. This method offers advantages of being versatility, cost effective, reproducible and environmental responsive as compared to the conventional methods.…”

mentioning

confidence: 99%

Development of aqueous two‐phase systems comprising poly ethylene glycol and dextran for purification of pullulan: Phase diagrams and fiscal analysis

Badhwar

Kumar

Dubey

2018

Engineering in Life Sciences

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Pullulan is a commercially important Exopolysaccharide (EPS) with wide‐spread applications which is produced by Aureobasidium pullulans. The alternative α (1 4) & α (1 6) configuration in pullulan provides it the specific structural and conformational properties. Pullulan is currently being exploited in food, health care, pharmacy, lithography, cosmetics. The fermented broth is processed by organic solvent precipitation for isolation and purification of pullulan. In this study, we have tried to analyze the potential of aqueous two phase system as an alternate technique to extract pullulan from fermented broth. Including this viability of ATPS was also compared with conventional organic solvent precipitation system in terms of cost and time. It was found that ATPS process produced a higher yield of pullulan (80.56%) than organic solvent precipitation method (71.6%). ATPS was also found more economical and less time consuming method.

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“…However, recent reports describe the use of aqueous two‐phase systems (ATPS) and three‐phase partitioning (TPP) as alternative downstream techniques for purifying invertase. An ATPS is a liquid–liquid system formed by mixing two water‐soluble substances, often polymers, that upon reaching certain critical concentrations, become immiscible with one another, leading to the formation of two phases . By contrast, TPP uses t‐butanol and ammonium sulfate to precipitate enzymes and proteins from aqueous solutions, forming three phases: a lower aqueous phase (water and ammonium sulfate), an upper t‐butanol‐rich phase, and, depending on the presence of contaminant proteins in the mixture and the concentration of ammonium sulfate used, an intermediate third phase (precipitate formed by protein or residual biomass) between the lower and upper phases .…”

Section: Introductionmentioning

confidence: 99%

“…An ATPS is a liquid-liquid system formed by mixing two water-soluble substances, often polymers, that upon reaching certain critical concentrations, become immiscible with one another, leading to the formation of two phases. 16,17 By contrast, TPP uses t-butanol and ammonium sulfate to precipitate enzymes and proteins from aqueous solutions, forming three phases: a lower aqueous phase (water and ammonium sulfate), an upper t-butanol-rich phase, and, depending on the presence of contaminant proteins in the mixture and the concentration of ammonium sulfate used, an intermediate third phase (precipitate formed by protein or residual biomass) between the lower and upper phases. 18,19 ATPS is considered to be less expensive and time-consuming than chromatography because it saves resources by integrating and intensifying processes, but usually the amount of sample loaded into ATPS is a small fraction of the complete system.…”

Section: Introductionmentioning

confidence: 99%

Monte Carlo economic analysis of Baker's yeast invertase purification using two‐ and three‐phase partitioning

Torres‐Acosta

Morales‐Guzman

Ruiz‐Ruiz

et al. 2018

J of Chemical Tech & Biotech

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BACKGROUND Invertase use in the food industry is limited by production costs. Alternative strategies for extraction, such as aqueous two‐phase systems (ATPS) and three‐phase partitioning (TPP), could be economically feasible for yeast invertase. Economic modeling of bioprocesses makes possible the identification of critical parameters for production costs and emulation of real scenarios, moreover incorporation of uncertainty is possible. This study performed an economic analysis on the production of invertase using ATPS or TPP, also a virtual optimization of ATPS was done. RESULTS TPP provided a lower production cost than ATPS ($145 vs $59.3 per 1 million enzymatic units, respectively). The critical parameter for TPP is recovery yield as it is highly dependent on operating conditions. In contrast, ATPS is dependent on materials costs as the sample load is smaller for ATPS, requiring a larger system. Although TPP provided a lower cost, t‐butanol hinders its acceptance. CONCLUSION Virtual optimization of ATPS found that varying sample load or system size is not enough to have a lower production cost than TPP, but provided insights for reduction of production costs and development of a safer technique. This study provides a framework for the virtual analysis of ATPS and TPP to evaluate their processes and reduce production costs. © 2018 Society of Chemical Industry

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Current applications of different type of aqueous two-phase systems

Cited by 91 publications

References 98 publications

Economic analysis of pilot‐scale production of B‐phycoerythrin

Economic analysis of pilot‐scale production of B‐phycoerythrin

Development of aqueous two‐phase systems comprising poly ethylene glycol and dextran for purification of pullulan: Phase diagrams and fiscal analysis

Monte Carlo economic analysis of Baker's yeast invertase purification using two‐ and three‐phase partitioning

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