Direct Radiation Effects on the Structure and Stability of Collagen and Other Proteins

Lalande, Mathieu; Schwob, Lucas; Vizcaino, Violaine; Chirot, Fabien; Dugourd, Philippe; Schlathölter, Thomas; Poully, Jean‐Christophe

doi:10.1002/cbic.201900202

Cited by 21 publications

(16 citation statements)

References 110 publications

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“…Organic solvents or detergents will alter hydrophobic interactions, and stability of enzymes in organic solvents will be dependent on the ability of the solvent to strip the essential hydration layer from the protein surface [25,26]. Last but not least, mechanical stress or radiation may disrupt the delicate balance of forces that maintain protein structure [27]. Stabilizing the enzyme means preventing these changes.…”

Section: Intrinsic (In)stability Of Enzymesmentioning

confidence: 99%

From Enzyme Stability to Enzymatic Bioelectrode Stabilization Processes

et al. 2021

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Bioelectrocatalysis using redox enzymes appears as a sustainable way for biosensing, electricity production, or biosynthesis of fine products. Despite advances in the knowledge of parameters that drive the efficiency of enzymatic electrocatalysis, the weak stability of bioelectrodes prevents large scale development of bioelectrocatalysis. In this review, starting from the understanding of the parameters that drive protein instability, we will discuss the main strategies available to improve all enzyme stability, including use of chemicals, protein engineering and immobilization. Considering in a second step the additional requirements for use of redox enzymes, we will evaluate how far these general strategies can be applied to bioelectrocatalysis.

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Section: Intrinsic (In)stability Of Enzymesmentioning

confidence: 99%

From Enzyme Stability to Enzymatic Bioelectrode Stabilization Processes

et al. 2021

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“…In contrast to UVR, photons of ionising radiation are energetic enough to ionise most molecules and atoms [ 92 ], potentially leading to the disruption of intermolecular bonds [ 93 ]. An abundance of water molecules in biological systems results in a large percentage of ionising radiation being absorbed by water in a process called water radiolysis [ 94 ], producing multiple ROS species.…”

Section: Ionising Radiation (X-rays/gamma Rays)mentioning

confidence: 99%

“…UVR can also damage the ECM via acellular pathways. Whilst degradation and breakdown of the triple helical structure of collagen can be mediated by photodynamically produced ROS [ 93 , 105 ], high UVR doses and/or non-environmentally attainable wavelengths are commonly required to induce measurable structural and functional effects on fibrillar collagens [ 124 ]. We have previously shown [ 74 , 76 , 146 ] that ECM proteins that are particularly enriched in UV chromophore amino acid residues are susceptible to UVR-induced degradation compared with UV chromophore-poor proteins such as collagen I and elastin.…”

Section: Model Tissue Systems For Radiation Studiesmentioning

confidence: 99%

“…Collectively, this ECM-remodelling has a detrimental impact on the mechanical strength and elasticity of the skin. Specifically, collagen degradation decreases the mechanical tension and stiffness of the ECM, which is hypothesised to reduce the size of fibroblasts, which are less able to exert a traction force on the ECM [ 93 , 139 ]. However, small doses of UVR have also been shown to increase the thermal stability of collagen [ 71 ], possibly attributed to the cross-linking of collagen fibres.…”

Section: Model Tissue Systems For Radiation Studiesmentioning

confidence: 99%

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The Effects of Ionising and Non-Ionising Electromagnetic Radiation on Extracellular Matrix Proteins

Tuieng

Cartmell

Kirwan

et al. 2021

Cells

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Exposure to sub-lethal doses of ionising and non-ionising electromagnetic radiation can impact human health and well-being as a consequence of, for example, the side effects of radiotherapy (therapeutic X-ray exposure) and accelerated skin ageing (chronic exposure to ultraviolet radiation: UVR). Whilst attention has focused primarily on the interaction of electromagnetic radiation with cells and cellular components, radiation-induced damage to long-lived extracellular matrix (ECM) proteins has the potential to profoundly affect tissue structure, composition and function. This review focuses on the current understanding of the biological effects of ionising and non-ionising radiation on the ECM of breast stroma and skin dermis, respectively. Although there is some experimental evidence for radiation-induced damage to ECM proteins, compared with the well-characterised impact of radiation exposure on cell biology, the structural, functional, and ultimately clinical consequences of ECM irradiation remain poorly defined.

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“…Third, the type and strength of ionizing radiation play a crucial role. The plausible mechanisms and molecular outcomes of ionizing radiation on proteins have been thoroughly examined in [18].…”

Section: Introductionmentioning

confidence: 99%

Optimization of Novel Human Acellular Dermal Dressing Sterilization for Routine Use in Clinical Practice

Lewandowska

Eljaszewicz

Poplawska

et al. 2021

IJMS

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Gamma rays and electrons with kinetic energy up to 10 MeV are routinely used to sterilize biomaterials. To date, the effects of irradiation upon human acellular dermal matrices (hADMs) remain to be fully elucidated. The optimal irradiation dosage remains a critical parameter affecting the final product structure and, by extension, its therapeutic potential. ADM slides were prepared by various digestion methods. The influence of various doses of radiation sterilization using a high-energy electron beam on the structure of collagen, the formation of free radicals and immune responses to non-irradiated (native) and irradiated hADM was investigated. The study of the structure changes was carried out using the following methods: immunohistology, immunoblotting, and electron paramagnetic resonance (EPR) spectroscopy. It was shown that radiation sterilization did not change the architecture and three-dimensional structure of hADM; however, it significantly influenced the degradation of collagen fibers and induced the production of free radicals in a dose-dependent manner. More importantly, the observed effects did not disrupt the therapeutic potential of the new transplants. Therefore, radiation sterilization at a dose of 35kGy can ensure high sterility of the dressing while maintaining its therapeutic potential.

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Direct Radiation Effects on the Structure and Stability of Collagen and Other Proteins

Cited by 21 publications

References 110 publications

From Enzyme Stability to Enzymatic Bioelectrode Stabilization Processes

From Enzyme Stability to Enzymatic Bioelectrode Stabilization Processes

The Effects of Ionising and Non-Ionising Electromagnetic Radiation on Extracellular Matrix Proteins

Optimization of Novel Human Acellular Dermal Dressing Sterilization for Routine Use in Clinical Practice

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