Direct peptide profiling of single neurons by matrix‐assisted laser desorption–ionization mass spectrometry

Veelen, Peter A. van; Jiménez, Connie R.; Li, K. W.; Wildering, Willem C.; Geraerts, W.P.M.; Tjaden, U.R.; Greef, J. van der

doi:10.1002/oms.1210281229

Cited by 77 publications

(74 citation statements)

References 15 publications

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“…Even with the wealth of genomic information available, direct biochemical measures are often required to determine the presence and particular form of the neuropeptides present throughout the central nervous system (CNS). Direct MALDI MS analysis of molluscan and insect neurons [1][2][3][4][5][6][7][8][9][10][11][12][13], connective nerve tissues [14], and even single peptidergic vesicles [15] provides information about gene products and their processing to bioactive neuropeptides. Mass profiling individually isolated neurons is the most common method for obtaining spatial information such as the cell-specific distribution of peptides within brain regions known as ganglia.…”

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confidence: 99%

Spatial profiling invertebrate ganglia using MALDI MS

Kruse

Sweedler

2003

J. Am. Soc. Mass Spectrom.

108

100

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The ability of MALDI TOF MS to spatially map peptides and proteins directly from a tissue is an exciting advance to imaging mass spectrometry. Recent advances in instrumentation for MS have resulted in instruments capable of achieving several micron spatial resolution while acquiring high-resolution mass spectra. Currently, the ability to obtain high quality mass spectrometric images depends on sample preparation protocols that often result in limited spatial resolution. A number of sample preparation and matrix deposition protocols are evaluated for spatial profiling of Aplysia californica exocrine gland and neuronal tissues. Such samples are different from mammalian tissues, but make good targets for method optimization because of the wealth of biochemical information available on neuropeptide processing and distribution. Electrospray matrix deposition and a variety of freezing methods have been found to be optimum for these invertebrate tissues,

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mentioning

confidence: 99%

Spatial profiling invertebrate ganglia using MALDI MS

Kruse

Sweedler

2003

J. Am. Soc. Mass Spectrom.

108

100

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“…[18][19][20][21][22] After our challenges using this method, we, however, noticed the following problems in applications: 1) Cells were killed by the addition of a matrix solution, and it is hard to trace a tiny target single cell on the evaporation and crystallization process of the matrix. 21 It is also hard to irradiate a laser beam to the point a target single cell in the congestion of micro-crystals of the matrix.…”

Section: Single Cell Analyses By Maldi-tof/msmentioning

confidence: 99%

Live Single-cell Mass Spectrometry

Masujima

2009

ANAL. SCI.

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The history from bio-imaging to live single-cell mass spectrometry (MS) is herein reviewed. The limitation of the current bio-imaging method is probing only known molecules, and a method for finding new molecules is needed for cells which, however, show individual behaviors even in the same incubation dish. Single-cell MALDI-TOF/MS has been developed, but it can detect only molecules that can be easily ionized, and not be exhaustive. Recently, the contents of a single cell have been sucked out by a nano-electro spray tip, and directly introduced into MS by nano-spray ionization. Thousands of molecular peaks have been successfully and exhaustively detected, and an extraction method for key molecules was also developed. This new method is now being widely applied to explore site-or state-specific molecules in various aspects of cell dynamisms.

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“…De l'analyse directe de tissu par spectrométrie de masse vers l'imagerie MALDI Depuis longtemps évoquée, la faisabilité de l'analyse directe de tissus biologiques par spectrométrie de masse de type MALDI-TOF a été démontrée pour la première fois en 1993 par l'équipe du Pr van der Greef grâce à des travaux sur des neurones de Lymnaea stagnalis [5]. Il a fallu attendre 1996 et la reprise de ces travaux par le groupe de Jonathan Sweedler [6] puis les déve-loppements de l'équipe de Richard Caprioli [7] pour que le profiling de tissu par spectrométrie de masse voit le jour.…”

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