Imagerie MALDI

Wisztorski, Maxence; Lemaire, R.; Stauber, Jonathan; Menguellet, Sonia Ait; Jardin-Mathé, Olivia; Day, Robert; Salzet, Michel; Fournier, Isabelle

doi:10.1051/medsci/2007231s31

Cited by 14 publications

(3 citation statements)

References 27 publications

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“…Thus, SIMS cannot be used to distinguish between single polymeric homologues in one sample. MALDI imaging mass spectrometry (IMS) has shown great promise as a mass‐specific method of analyzing biological tissue in such diverse areas as drug dosing and cancer boundary detection studies 24–27…”

mentioning

confidence: 99%

Imaging mass spectrometry for examining localization of polymeric composition in matrix‐assisted laser desorption/ionization samples

Weidner

Falkenhagen

2009

Rapid Comm Mass Spectrometry

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The localization of polymeric composition in samples prepared for matrix-assisted laser desorption/ionization (MALDI) analysis has been investigated by imaging mass spectrometry. Various matrices and solvents were used for sample spot preparation of a polybutyleneglycol (PBG 1000). It was shown that in visibly homogeneous spots, prepared using the 'dried droplet' method, separation between matrix and polymer takes place. Moreover, using alpha-cyano-4-hydroxycinnamic acid (CCA) as matrix and methanol as solvent molecular mass separation of the polymer homologues in the spots was detectable. In contrast to manually spotted samples, dry spray deposition results in homogeneous layers showing no separation effects.

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mentioning

confidence: 99%

Imaging mass spectrometry for examining localization of polymeric composition in matrix‐assisted laser desorption/ionization samples

Weidner

Falkenhagen

2009

Rapid Comm Mass Spectrometry

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“…Derivatization protocols have been applied to detect drugs, neurotransmitters, , and endogenous neutral steroids . Finally, another area that could be further developed is photocleavable mass-tag technologies such as those used to probe tissues. , A small molecule mass-tag approach is less complex than an untargeted, full spectrum analysis and may thus be useful in enabling the future analysis of larger molecular weight biologics.…”

Section: Future Perspectivesmentioning

confidence: 99%

Mass Spectrometry Imaging in Drug Development

et al. 2015

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“…MSI can provide new directions for clinical proteomic studies, especially in the Neurosciences, with the joint goals of characterizing cellular circuitry and understanding the impact of disease and therapy on cellular networks through the analysis of pathological tissue samples (Wisztorski et al, 2007a). Of particular relevance to the focus of this special issue of Developmental Neurobiology, MSI has already been demonstrated to be a valuable technique for exploring the molecular components of neural structures, as discussed in the next two sections.…”

Section: Mass Spectrometry Imaging (Msi)mentioning

confidence: 99%

Molecular MALDI imaging: An emerging technology for neuroscience studies

Wisztorski

Croix

Macagno

et al. 2008

Developmental Neurobiology

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Mass spectrometry (MS) has become an essential tool for the detection, identification, and characterization of the molecular components of biological processes, such as those responsible for the dynamic properties of the nervous system. Generally, the application of these powerful techniques requires the destruction of the specimen under study, but recent technological advances have made it possible to apply the matrix-assisted laser desorption/ionization (MALDI) MS technique directly to tissue sections. The major advantage of direct MALDI analysis is that it enables the acquisition of local molecular expression profiles, while maintaining the topographic integrity of the tissue and avoiding time-consuming extraction, purification, and separation steps, which have the potential for introducing artifacts. With automation and the ability to display complex spectral data using imaging software, it is now possible to create multiple 2D maps of selected biomolecules in register with tissue sections, a method now known as MALDI Imaging, or MSI (for Mass Spectrometry Imaging). This creates, for example, an opportunity to correlate functional states, determined a priori with live recording or imaging, with the corresponding molecular maps obtained at the time the tissue is frozen and analyzed with MSI. We review the increasing application of MALDI Imaging to the analysis of molecular distributions of proteins and peptides in nervous tissues of both vertebrates and invertebrates, focusing in particular on recent studies of neurodegenerative diseases and early efforts to implement assays of neuronal development.

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Imagerie MALDI

Cited by 14 publications

References 27 publications

Imaging mass spectrometry for examining localization of polymeric composition in matrix‐assisted laser desorption/ionization samples

Imaging mass spectrometry for examining localization of polymeric composition in matrix‐assisted laser desorption/ionization samples

Mass Spectrometry Imaging in Drug Development

Molecular MALDI imaging: An emerging technology for neuroscience studies

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