Direct Optical Imaging of Graphene In Vitro by Nonlinear Femtosecond Laser Spectral Reshaping

Li, Baolei; Cheng, Yingwen; Liu, Jie; Yi, Congwen; Brown, April S.; Yuan, Hsiangkuo; Vo‐Dinh, Tuan; Fischer, Martin C.; Warren, Warren S.

doi:10.1021/nl303358p

Cited by 30 publications

(21 citation statements)

References 38 publications

(70 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Since the discoveries of fullerene C 60 in 1985 1 , carbon nanotubes (CNTs) in 1991 2 , and graphene in 2004 3 , carbon-based nanomaterials (CBNs) have attracted considerable attention in various biomedical fields 4 5 (including those related to gene delivery 6 , optical imaging 7 , and nanotherapeutics) 8 9 10 11 12 because of their excellent mechanical, optical, and electrical properties 13 14 15 . However, growing concerns over pathologies CBNs might effect on human patients have established the study of nanotoxicity as an important element of nanoscience and nanotechnology 16 17 18 19 .…”

mentioning

confidence: 99%

Surface Curvature Relation to Protein Adsorption for Carbon-based Nanomaterials

Yang

Chong

et al. 2015

Sci Rep

104

View full text Add to dashboard Cite

The adsorption of proteins onto carbon-based nanomaterials (CBNs) is dictated by hydrophobic and π-π interactions between aliphatic and aromatic residues and the conjugated CBN surface. Accordingly, protein adsorption is highly sensitive to topological constraints imposed by CBN surface structure; in particular, adsorption capacity is thought to increase as the incident surface curvature decreases. In this work, we couple Molecular Dynamics (MD) simulations with fluorescence spectroscopy experiments to characterize this curvature dependence in detail for the model protein bovine serum albumin (BSA). By studying BSA adsorption onto carbon nanotubes of increasing radius (featuring descending local curvatures) and a flat graphene sheet, we confirm that adsorption capacity is indeed enhanced on flatter surfaces. Naïve fluorescence experiments featuring multi-walled carbon nanotubes (MWCNTs), however, conform to an opposing trend. To reconcile these observations, we conduct additional MD simulations with MWCNTs that match those prepared in experiments; such simulations indicate that increased mass to surface area ratios in multi-walled systems explain the observed discrepancies. In reduction, our work substantiates the inverse relationship between protein adsorption capacity and surface curvature and further demonstrates the need for subtle consideration in experimental and simulation design.

show abstract

mentioning

confidence: 99%

Surface Curvature Relation to Protein Adsorption for Carbon-based Nanomaterials

Yang

Chong

et al. 2015

Sci Rep

104

View full text Add to dashboard Cite

show abstract

“…Fluorescence quenching microscopy was recently employed to quickly visualize graphene based sheets, where a fluorescent dye was coated on the surface22. A nonlinear optical microscopy method based on self-phase modulation was used for in vitro imaging of graphene, but this contrast is not sensitive to GO23.…”

mentioning

confidence: 99%

Highly sensitive transient absorption imaging of graphene and graphene oxide in living cells and circulating blood

Zhang

Chung

et al. 2015

Sci Rep

View full text Add to dashboard Cite

We report a transient absorption (TA) imaging method for fast visualization and quantitative layer analysis of graphene and GO. Forward and backward imaging of graphene on various substrates under ambient condition was imaged with a speed of 2 μs per pixel. The TA intensity linearly increased with the layer number of graphene. Real-time TA imaging of GO in vitro with capability of quantitative analysis of intracellular concentration and ex vivo in circulating blood were demonstrated. These results suggest that TA microscopy is a valid tool for the study of graphene based materials.

show abstract

“…Peng et al demonstrated that the photoluminescence of the nano‐GO can be tailored through varying the size of the nano‐sheets 139. The application of this technique to cancer cell imaging has already been investigated with different types of cells, gastric cancer cell line (AGS),140 BT549 breast cancer cells line141 and MG‐63 human osteosarcoma cell line135. Photoluminescence of nano‐GO was also investigated along with the potential application on photothermal cancer cell therapy.…”

Section: Key Points Of Nano‐go On Cancer Therapymentioning

confidence: 99%

Nano‐Graphene Oxide: A Potential Multifunctional Platform for Cancer Therapy

Gonçalves

Vila

Portolés

et al. 2013

Adv Healthcare Materials

158

View full text Add to dashboard Cite

Nano-GO is a graphene derivative with a 2D atomic layer of sp² bonded carbon atoms in hexagonal conformation together with sp³ domains with carbon atoms linked to oxygen functional groups. The supremacy of nano-GO resides essentially in its own intrinsic chemical and physical structure, which confers an extraordinary chemical versatility, high aspect ratio and unusual physical properties. The chemical versatility of nano-GO arises from the oxygen functional groups on the carbon structure that make possible its relatively easy functionalization, under mild conditions, with organic molecules or biological structures in covalent or non-covalent linkage. The synergistic effects resulting from the assembly of well-defined structures at nano-GO surface, in addition to its intrinsic optical, mechanical and electronic properties, allow the development of new multifunctional hybrid materials with a high potential in multimodal cancer therapy. Herein, a comprehensive review of the fundamental properties of nano-GO requirements for cancer therapy and the first developments of nano-GO as a platform for this purpose is presented.

show abstract

Direct Optical Imaging of Graphene In Vitro by Nonlinear Femtosecond Laser Spectral Reshaping

Cited by 30 publications

References 38 publications

Surface Curvature Relation to Protein Adsorption for Carbon-based Nanomaterials

Surface Curvature Relation to Protein Adsorption for Carbon-based Nanomaterials

Highly sensitive transient absorption imaging of graphene and graphene oxide in living cells and circulating blood

Nano‐Graphene Oxide: A Potential Multifunctional Platform for Cancer Therapy

Contact Info

Product

Resources

About