Direct observation of surface charge and stiffness of human metaphase chromosomes

Roh, Seokbeom; Lee, Taeha; Cheong, Da Yeon; Kim, Yeon-Jin; Oh, Soohwan; Lee, Gyudo

doi:10.1039/d2na00620k

Cited by 3 publications

(4 citation statements)

References 60 publications

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“…We found that the VPA-treated chromosomes were strikingly 6 times more elastic than untreated chromosomes (12.5±2 MPa (untreated) vs. 2.0±0.6 (VPA-treated), p<0.01) ( Figure 4, F, I ). The range of Young’s modulus values of chromosomes were consistent with previous reports 29,45 . This data reveals how epigenetic reprogramming impact material properties of chromosomes.…”

Section: Resultssupporting

confidence: 92%

“…2.0±0.6 (VPA-treated), p<0.01) (Figure 4, F, I). The range of Young's modulus values of chromosomes were consistent with previous reports 29,45 . This data reveals how epigenetic reprogramming impact material properties of chromosomes.…”

Section: Resultssupporting

confidence: 91%

“…Lipiec et al applied AFM-Raman to reveal molecular alterations in Hela chromosomes by inducing bleomycin 27 , previously they studied molecular differences between eu-and heterochromatin using AFM-IR based on differences in methylation status 28 . In another work, Lee group evaluated the surface charge and stiffness of Human B lymphocyte chromosomes by treating them with RNase and pepsin enzymes using KPFM and PF-QNM techniques 29 . In this study, for the first time, we quantified both the single chromosome elasticity and electron transport through isolated MCF7 single metaphase chromosomes using AFM to decipher the correlation between the chromatin structural alterations induced by VPA and its therapeutic function.…”

Section: Centromerementioning

confidence: 99%

See 2 more Smart Citations

Valproic Acid Treatment Enhances Chromosome Flexibility and Electron Transport in MCF7 Breast Cancer Cells

Agrawal,

Paul,

Mishra

et al. 2024

Preprint

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The structural integrity of the chromosomes is essential to every functional process within the eukaryotic nuclei. Chromosomes are DNA-histone complexes essential for the inheritance of genetic information to the offspring and any defect in it is linked to mitotic errors, cancer growth, and cellular aging. Changes in the mechanical properties of a chromosome could lead to its compromised function and stability, leading to chromosome breaks. Here, we studied the changes in chromosome physical properties using metaphase chromosomes isolated from human breast cancer cells (MCF7) exposed to Valproic Acid (VPA), a known epigenetic modifier drug involved in histone hyperacetylation and DNA demethylation. Due to chromosomal structural intricacy, preparative and technical limitations of analytical tools, we employed a label-free atomic force microscopy approach for simultaneously visualizing and mapping single chromosome elasticity. Additionally, we performed electron transport characteristics of metaphase chromosomes to elucidate the effect of VPA. Our multi-parametric strategy of probing physical properties of chromosomes offers a new scope in terms of analytical tools for studying chromosomal structural changes/aberrations and associated structure-function relationships pertinent to cancer.

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Section: Resultssupporting

confidence: 92%

Section: Resultssupporting

confidence: 91%

Section: Centromerementioning

confidence: 99%

See 1 more Smart Citation

Valproic Acid Treatment Enhances Chromosome Flexibility and Electron Transport in MCF7 Breast Cancer Cells

Agrawal,

Paul,

Mishra

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…To remove nucleic acid and protein contaminants, the chromosomes were treated with RNase (Sigma-Aldrich, R6513) and protease (Sigma-Aldrich, P6887). The purification was performed following a protocol developed earlier by Roh et al 31 Before performing further experiments chromosomes were routinely checked under an optical microscope (Scheme S1 and Figure S1). 5.…”

Section: ■ Introductionmentioning

confidence: 99%

Label-Free Physical–Analytical Techniques Reveal Epigenetic Modifications of Breast Cancer Chromosomes

Agrawal¹,

Paul²,

Saroj³

et al. 2023

J. Phys. Chem. B

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Epigenetic dysregulation including DNA methylation and histone modifications is being increasingly recognized as a promising biomarker for the diagnosis and prognosis of cancer. Herein, we devised a label-free analytical toolbox comprising IR, UV–vis, CD spectroscopy, and cyclic voltammetry, which is capable to differentiate significantly hyper-methylated breast cancer chromosomes from the normal breast epithelial counterparts.

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Molecular alterations in metaphase chromosomes induced by bleomycin

Urbańska,

Sofińska,

Czaja

et al. 2024

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

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Direct observation of surface charge and stiffness of human metaphase chromosomes

Abstract: Metaphase chromosomes in which both polynucleotides and proteins are condensed with hierarchies are closely related to life phenomena such as cell division, cancer development, and cellular senescence. Nevertheless, their nature...

Cited by 3 publications

References 60 publications

Valproic Acid Treatment Enhances Chromosome Flexibility and Electron Transport in MCF7 Breast Cancer Cells

Valproic Acid Treatment Enhances Chromosome Flexibility and Electron Transport in MCF7 Breast Cancer Cells

Label-Free Physical–Analytical Techniques Reveal Epigenetic Modifications of Breast Cancer Chromosomes

Molecular alterations in metaphase chromosomes induced by bleomycin

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