Direct observation of peptide hydrogel self-assembly

Adams, Zoë C.; Olson, Erika; Lopez-Silva, Tania L.; Lian, Zhengwen; Kim, Audrey Y.; Holcomb, Matthew; Zimmermann, Jörg; Adhikary, Ramkrishna; Dawson, Philip

doi:10.1039/d1sc06562a

Cited by 7 publications

(9 citation statements)

References 66 publications

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“…Techniques that study infrared absorption, circular dichroism, or fluorescence spectra are able to capture time‐resolved structural changes of biomolecules [45,46] . In addition to quantifying the concentration of a given analyte, spectroscopic methods report on changes in the molecular conformation, [46] environment, [30] and bonding [47] of the analyte. Optical spectroscopy methods may also be enhanced by coupling them with other tools such as pump‐probe, [23] stopped‐flow, [47] or confocal microscopy [30] …”

Section: Approaches To In Situ Characterization Of Biomolecular Assemblymentioning

confidence: 99%

“…In the case of proteins, CD spectra can be fit to extract the proportion of structural motifs, such as β-sheets and α-helices, present in the sample. Time-resolved CD has been used to study the self-assembly of protein structures such as amyloid fibrils, [46] chiral supramolecular structures and hydrogels, [47,[63][64][65] and protein-surfactant complexes. [66] A typical CD spectrum requires several minutes to acquire.…”

Section: Circular Dichroism Spectroscopymentioning

confidence: 99%

“…The vibrational mode of a molecule is sensitive to both the bond strength and difference in mass between the two atoms. Selective deuteration experiments can exploit this mass dependence to probe specific bonds in a system, help with peak assignment, or study dynamics through the exchange of hydrogen and deuterium [47,49–51] . Adams et al [47] .…”

Section: Approaches To In Situ Characterization Of Biomolecular Assemblymentioning

confidence: 99%

“…Selective deuteration experiments can exploit this mass dependence to probe specific bonds in a system, help with peak assignment, or study dynamics through the exchange of hydrogen and deuterium. [47,[49][50][51] Adams et al [47] performed time-resolved stopped-flow FTIR studies on the gelation of MAX1, a peptide designed to mimic amphiphilic β-sheet-containing peptides that is being explored in therapeutic hydrogel applications. They designed five variants, replacing one of five valines with a perdeuterated valine to separate the absorption of the CÀ D peak from other species in the system.…”

Section: Fourier Transform Infrared Spectroscopy (Ftir) and Raman Spe...mentioning

confidence: 99%

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Mechanisms of Biomolecular Self‐Assembly Investigated Through In Situ Observations of Structures and Dynamics

Schmid,

Lachowski,

Chiang

et al. 2023

Angew Chem Int Ed

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Biomolecular self‐assembly of hierarchical materials is a precise and adaptable bottom‐up approach to synthesizing across scales with considerable energy, health, environment, sustainability, and information technology applications. To achieve desired functions in biomaterials, it is essential to directly observe assembly dynamics and structural evolutions that reflect the underlying energy landscape and the assembly mechanism. This review will summarize the current understanding of biomolecular assembly mechanisms based on in situ characterization and discuss the broader significance and achievements of newly gained insights. In addition, we will also introduce how emerging deep learning/machine learning‐based approaches, multiparametric characterization, and high‐throughput methods can boost the development of biomolecular self‐assembly. The objective of this review is to accelerate the development of in situ characterization approaches for biomolecular self‐assembly and to inspire the next generation of biomimetic materials.

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Section: Approaches To In Situ Characterization Of Biomolecular Assemblymentioning

confidence: 99%

Section: Circular Dichroism Spectroscopymentioning

confidence: 99%

Section: Approaches To In Situ Characterization Of Biomolecular Assemblymentioning

confidence: 99%

Section: Fourier Transform Infrared Spectroscopy (Ftir) and Raman Spe...mentioning

confidence: 99%

See 2 more Smart Citations

Mechanisms of Biomolecular Self‐Assembly Investigated Through In Situ Observations of Structures and Dynamics

Schmid,

Lachowski,

Chiang

et al. 2023

Angew Chem Int Ed

View full text Add to dashboard Cite

show abstract

“…[64][65][66][67] Various nanostructures of self-assembled peptide materials, such as hydrogels, [68][69][70][71] lipid-like vesicles, 72 and nanofibers, [73][74][75] are ideal candidates for being developed into drug carriers due to their superb packaging ability, safety protection, and precise drug release. Compared to other traditional nano-drug systems, the main advantage of peptide scaffold-based smart nano-drug systems is that they can respond to multi-layered stimuli in vivo, such as microenvironmental stimuli, cellular stimuli (e.g., pH, 76 ionic strength, [77][78][79] receptor-ligand interactions, [80][81][82] specific proteins, 83 or enzymes 84,85 ), and external environmental stimuli (e.g., light, 86 temperature, 87 ultrasound, 88 electric, 89 or magnetic fields 90 ), and can mediate controlled drug release in situ. This mechanism with a dynamic response similar to a carefully designed "autonomous cruise missile" maximizes the killing of tumor targets while avoiding damage to non-target organs, increasing loaded drug bioavailability.…”

mentioning

confidence: 99%

Dynamic responsiveness of self‐assembling peptide‐based nano‐drug systems

Wang

Zhan

Huang

et al. 2023

Interdisciplinary Medicine

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Dynamic‐responsive self‐assembly is the process of ordered supramolecular structure formation or reversible decomposition from building blocks. This process is driven by non‐covalent interactions based on complex stimulus‐responsive systems comprising different components within a microenvironment. Furthermore, stimuli‐responsive assembly‐disassembly is an intrinsic interaction process in organisms, indispensable in maintaining life activities and functions. However, the dynamic interactions between dynamically responsive nano‐drug systems (DRNSs) and biological systems remain unpredictable, which are a challenge for the precisely targeted therapy and controlled drug release of DRNSs in vivo. This review highlights novel self‐assembling peptide‐based nano‐drug systems and their biological interactions. By precisely controlling the shape and size of self‐assembled peptide nanomaterials, biologically simulated components with diverse biological functions and precise transport at the subcellular level can be achieved. We have also summarized the limitations and challenges of responsive self‐assembling peptide nanomaterials in clinical translation. Additionally, we have discussed the future perspectives of supramolecular therapeutics using signaling molecule gradient concentrations and efficiencies and highlighted the direction for developing clinically translatable smart nanomedicines.

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Short Peptide Nanofiber Biomaterials Ameliorate Local Hemostatic Capacity of Surgical Materials and Intraoperative Hemostatic Applications in Clinics

et al. 2023

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Short designer self‐assembling peptide (dSAP) biomaterials are a new addition to the hemostat group. It may provide a diverse and robust toolbox for surgeons to integrate wound microenvironment with much safer and stronger hemostatic capacity than conventional materials and hemostatic agents. Especially in noncompressible torso hemorrhage (NCTH), diffuse mucosal surface bleeding, and internal medical bleeding (IMB), with respect to the optimal hemostatic formulation, dSAP biomaterials are the ingenious nanofiber alternatives to make bioactive neural scaffold, nasal packing, large mucosal surface coverage in gastrointestinal surgery (esophagus, gastric lesion, duodenum, and lower digestive tract), epicardiac cell‐delivery carrier, transparent matrix barrier, and so on. Herein, in multiple surgical specialties, dSAP‐biomaterial‐based nano‐hemostats achieve safe, effective, and immediate hemostasis, facile wound healing, and potentially reduce the risks in delayed bleeding, rebleeding, post‐operative bleeding, or related complications. The biosafety in vivo, bleeding indications, tissue‐sealing quality, surgical feasibility, and local usability are addressed comprehensively and sequentially and pursued to develop useful surgical techniques with better hemostatic performance. Here, the state of the art and all‐round advancements of nano‐hemostatic approaches in surgery are provided. Relevant critical insights will inspire exciting investigations on peptide nanotechnology, next‐generation biomaterials, and better promising prospects in clinics.

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Direct observation of peptide hydrogel self-assembly

Abstract: To facilitate the characterization of phase-transitioning molecules, site-specific non-perturbative infrared probes are leveraged for continuous observation of the self-assembly of fibrils in a peptide hydrogel following stopped-flow initiation.

Cited by 7 publications

References 66 publications

Mechanisms of Biomolecular Self‐Assembly Investigated Through In Situ Observations of Structures and Dynamics

Mechanisms of Biomolecular Self‐Assembly Investigated Through In Situ Observations of Structures and Dynamics

Dynamic responsiveness of self‐assembling peptide‐based nano‐drug systems

Short Peptide Nanofiber Biomaterials Ameliorate Local Hemostatic Capacity of Surgical Materials and Intraoperative Hemostatic Applications in Clinics

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