Direct measurement of Raman spectra of intact lens in a whole eyeball

Mizuno, A; Ozaki, Yukihiro; Kamada, Yoshio; Miyazaki, Hitoshi; Itoh, Koichi; Iriyama, Keiji

doi:10.3109/02713688109001862

Cited by 17 publications

(9 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Mineralized and collageneous tissues such as dentine (Rippon et al 1971), bovine Achilles tendon (Freshour and Koenig 1975), cornea (Goheen et al 1978) and ocular lens (Farrell andMcCauley 1976, Yu et al 1985) were the first complex tissue matrices to be studied by visibleexcitation Raman spectroscopy, because they exhibit relatively little fluorescence background. Raman measurements of intact lenses have been reported by many groups (Mizuno et al 1981, Yu et al 1982, Bot et al 1989. Protein aggregation and pigmentation that occur during lens aging and cataract formation were studied by Ozaki et al (1989).…”

Section: Visible Excitationmentioning

confidence: 99%

Prospects forin vivoRaman spectroscopy

et al. 2000

View full text Add to dashboard Cite

Raman spectroscopy is a potentially important clinical tool for real-time diagnosis of disease and in situ evaluation of living tissue. The purpose of this article is to review the biological and physical basis of Raman spectroscopy of tissue, to assess the current status of the field and to explore future directions. The principles of Raman spectroscopy and the molecular level information it provides are explained. An overview of the evolution of Raman spectroscopic techniques in biology and medicine, from early investigations using visible laser excitation to present-day technology based on near-infrared laser excitation and charge-coupled device array detection, is presented. State-of-the-art Raman spectrometer systems for research laboratory and clinical settings are described. Modern methods of multivariate spectral analysis for extracting diagnostic, chemical and morphological information are reviewed. Several in-depth applications are presented to illustrate the methods of collecting, processing and analysing data, as well as the range of medical applications under study. Finally, the issues to be addressed in implementing Raman spectroscopy in various clinical applications, as well as some long-term directions for future study, are discussed.

show abstract

Section: Visible Excitationmentioning

confidence: 99%

Prospects forin vivoRaman spectroscopy

et al. 2000

View full text Add to dashboard Cite

show abstract

“…The 758 and 881-cm Ϫ1 bands have been assigned to tryptophan. 4,5,15 The 852-cm Ϫ1 Raman band is from tyrosine. 5,15 The 1003 and 1031-cm Ϫ1 bands have been assigned to phenylalanine.…”

Section: Raman Spectra From Porcine Whole Eyesmentioning

confidence: 99%

“…Yu, Kuck, and Askren 4 measured and characterized the Raman spectrum from the intact rabbit lens. Mizuno et al directly measured the Raman spectra from intact rabbit and rat lenses, 5 and used near infrared Fourier transform spectroscopy to measure the Raman spectrum from rabbit cornea and sclera. 6 Sebag et al 7 employed Raman spectroscopy to investigate changes in the vitreous introduced by diabetes.…”

Section: Introductionmentioning

confidence: 99%

Detection of glutamate in the eye by Raman spectroscopy

et al. 2003

View full text Add to dashboard Cite

Raman spectroscopy is used to detect glutamate in the eye. Glutamate, a by-product of nerve cell death, is an indicator of glaucoma and diabetic retinopathy. The Raman spectra of ex vivo whole porcine eyes and individual components (lens, cornea, vitreous) are measured and characterized. Monosodium glutamate is injected into the eyes to simulate disease conditions, and the contribution to the Raman spectrum due to the presence of glutamate is identified. The Raman spectra from the native eye is dominated by vibrational modes from proteins in the lens. An optical system is designed to optimize collection of signal from the vitreous, where the glutamate is located, and reduce the Raman from the lens. Two vibrational fingerprints of monosodium glutamate are detected at 1369 and 1422 cm(-1), although the concentrations are much above physiological concentrations.

show abstract

“…Intact rabbit and rat lenses were measured intraocularly by Mizuno et al [143]. The development of a high-fidelity Raman imaging microscope by Goldstein et al [144] o#ers potential in situ applications to investigate the lens.…”

Section: In Situ Studiesmentioning

confidence: 99%

Raman Spectroscopy in Ophthalmology: From Experimental Tool to Applications In Vivo

et al. 2001

View full text Add to dashboard Cite

Raman spectroscopy is a qualitative and quantitative optical technique for determining the molecular composition of matter. Improvements in spectroscopic instruments, especially the modality to detect low light level signals extended the Raman technique to biomedical applications, even in delicate structures like the eye. The purpose of this paper was to make an inventory of performed applications of Raman spectroscopy in biomedical science and especially in ophthalmology. A literature search was done using Medline, Current Contents, a patent server on the Internet, and references found in articles and patents. This search revealed a variety of Raman techniques and applications in biomedical research, and an increasing flow of articles starting in the late 1970s on Raman spectroscopy in ophthalmology. This increase in literature about Raman spectroscopy in ophthalmology feeds the expectation that this valuable technique will be introduced in the future into clinical practice.

show abstract

Direct measurement of Raman spectra of intact lens in a whole eyeball

Cited by 17 publications

References 8 publications

Prospects forin vivoRaman spectroscopy

Prospects forin vivoRaman spectroscopy

Detection of glutamate in the eye by Raman spectroscopy

Raman Spectroscopy in Ophthalmology: From Experimental Tool to Applications In Vivo

Contact Info

Product

Resources

About