Detection of glutamate in the eye by Raman spectroscopy

Katz, Alvin; Kruger, Erik F.; Minko, Glenn; Liu, C. H.; Rosen, Richard B.; Alfano, R. R.

doi:10.1117/1.1559726

Cited by 30 publications

(14 citation statements)

References 13 publications

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“…In addition, compound-specific molecules can be detected from a specimen with less spectral interference from water. Raman spectroscopy is now being widely used in the field of biophysics to obtain molecular structure information [25,26] as well as disease diagnosis both in vivo and in vitro [23,24,27] . In this paper, the molecular/structural differences in both inorganic and organic phases between normal and NCCL dentin were investigated using Raman microspectroscopic technique.…”

Section: Introductionmentioning

confidence: 99%

Molecular structural analysis of noncarious cervical sclerotic dentin using Raman spectroscopy

Karan

Yao

et al. 2009

J Raman Spectroscopy

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Molecular structure of the sclerotic dentin in noncarious cervical lesions (NCCLs) including both the inorganic phase and organic phase was investigated using Raman spectroscopy. It was found that NCCL sclerotic dentin was hypermineralized with the mineral/matrix ratios 2-3 times higher than those of normal dentin, which was caused by both the increase of mineral content and decrease of organic matrix (collagen) content in the sclerotic dentin. For the inorganic phase, the phosphate band (PO 4 3− , ν 1 , symmetric stretching vibrational mode) in NCCL sclerotic dentin was shifted from 960 to 963 cm −1 , and the width of this band was decreased from 16.4 to 10.4 cm −1 , indicating that the degree of mineral crystallinity in NCCL sclerotic dentin was higher than that of normal dentin. In addition, the carbonate content in the mineral of NCCL sclerotic dentin was less than that of normal dentin. As compared to the inorganic phase, the changes within the organic phase were not dramatic. However, the changes in collagen cross-link density along with other spectral changes were still detectable. There was a noteworthy reduction in the ratio of nonreducible to reducible cross-links in the NCCL sclerotic dentin, indicating that cross-link breaks occurred in the collagen matrix of the lesions.

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Section: Introductionmentioning

confidence: 99%

Molecular structural analysis of noncarious cervical sclerotic dentin using Raman spectroscopy

Karan

Yao

et al. 2009

J Raman Spectroscopy

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“…Earlier reports utilizing Raman spectroscopy for ophthalmological investigation focused on detection of human macular pigment and glutamate in the eyes, in which the excitation laser wavelength was selected to resonate with the vibrational modes of the pigment/glutamate molecules, and an enhanced Raman signal was achieved. 25,27 To acquire a Raman signal directly from the RGC cells without the resonance enhancement effects, the autofluorescence background has to be removed before the analysis of the vibrational bands takes place. The most common methods for background removal, as the one currently used in this study, are based on digital signal subtraction utilizing the common feature of fluorescent backgrounds-a smooth function of the emission wavelength.…”

Section: Discussionmentioning

confidence: 99%

“…For the 780 nm diode laser that was used in this study, the threshold limit value (TLV) can be calculated as follows, 24,25 where CA is the correction factor for different wavelength:…”

Section: Discussionmentioning

confidence: 99%

Exploring Raman spectroscopy for the evaluation of glaucomatous retinal changes

Wang

Grozdanić²,

Harper³

et al. 2011

J. Biomed. Opt.

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Abstract. Glaucoma is a chronic neurodegenerative disease characterized by apoptosis of retinal ganglion cells and subsequent loss of visual function. Early detection of glaucoma is critical for the prevention of permanent structural damage and irreversible vision loss. Raman spectroscopy is a technique that provides rapid biochemical characterization of tissues in a nondestructive and noninvasive fashion. In this study, we explored the potential of using Raman spectroscopy for detection of glaucomatous changes in vitro. Raman spectroscopic imaging was conducted on retinal tissues of dogs with hereditary glaucoma and healthy control dogs. The Raman spectra were subjected to multivariate discriminant analysis with a support vector machine algorithm, and a classification model was developed to differentiate disease tissues versus healthy tissues. Spectroscopic analysis of 105 retinal ganglion cells (RGCs) from glaucomatous dogs and 267 RGCs from healthy dogs revealed spectroscopic markers that differentiated glaucomatous specimens from healthy controls. Furthermore, the multivariate discriminant model differentiated healthy samples and glaucomatous samples with good accuracy [healthy 89.5% and glaucomatous 97.6% for the same breed (Basset Hounds); and healthy 85.0% and glaucomatous 85.5% for different breeds (Beagles versus Basset Hounds)]. Raman spectroscopic screening can be used for in vitro detection of glaucomatous changes in retinal tissue with a high specificity. C 2011 Society of Photo-Optical Instrumentation Engineers (SPIE).

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“… Estudios in-vivo e in-vitro de la piel [11][12][13],  Estudios de transferencia trans-dérmica de fármacos [14],  Identificación de Cáncer (piel, oral, de mama, de cuello uterino, colo-rectal) [15][16][17][18][19],  Los estudios de hueso (composición, la mecánica y estado de la enfermedad) [20,21],  Dientes [22,23],  Aterosclerosis intra-coronaria [24],  Estudios en el ojo (cornea y fluidos) [25,26].…”

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Raman spectroscopy and its applications

Castro‐Ramos¹

2013

Opt. Pura Apl.

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RESUMEN:El presente artículo trata una de las técnicas espectroscópicas con mayor crecimiento en los últimos 20 años: la espectroscopia Raman. Se describen algunas de las aplicaciones más importantes que se conocen en el mundo hoy en día desde su origen así como la relación que ha tenido con los avances tecnológicos que han generado un alto impacto en la sociedad. Se explica de manera didáctica a fin de que estudiantes de bachillerato y licenciatura conozcan los fundamentos de la espectroscopia Raman.Palabras clave: Espectroscopia, Espectroscopia Raman. ABSTRACT:This paper addresses one of the fastest growing spectroscopic techniques over the past 20 years: Raman spectroscopy. It describes some of the most important applications that are known in the world nowadays from its origin and the relationship it had with the technological advances that have generated a high impact on society. It explains in a didactic way so that high school and undergraduate students learn the fundamentals of Raman spectroscopy.

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Detection of glutamate in the eye by Raman spectroscopy

Cited by 30 publications

References 13 publications

Molecular structural analysis of noncarious cervical sclerotic dentin using Raman spectroscopy

Molecular structural analysis of noncarious cervical sclerotic dentin using Raman spectroscopy

Exploring Raman spectroscopy for the evaluation of glaucomatous retinal changes

Raman spectroscopy and its applications

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