Direct measurement of phase coexistence in DPPC/cholesterol vesicles using Raman spectroscopy

Lange, M.J.L. de; Bonn, Mischa; Müller, Markus

doi:10.1016/j.chemphyslip.2006.12.007

Cited by 43 publications

(40 citation statements)

References 29 publications

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“…5,6 To explore membrane properties, bilayers made from synthetic lipids are oen used. [1][2][3][4]7 The heterogeneity of model membranes was studied with NMR and EPR, 1,2,[8][9][10] small-angle neutron scattering, 11,12 uorescence microscopy, 13,14 Raman scattering, [15][16][17][18][19][20] computer simulation, 21,22 and other techniques.…”

Section: Introductionmentioning

confidence: 99%

Normal vibrations of ternary DOPC/DPPC/cholesterol lipid bilayers by low-frequency Raman spectroscopy

2019

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Section: Introductionmentioning

confidence: 99%

Normal vibrations of ternary DOPC/DPPC/cholesterol lipid bilayers by low-frequency Raman spectroscopy

2019

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“…Considering the large number of studies performed on this mixture, it is puzzling how few of them have actually captured behaviour consistent with the coexistence of L o - and L d -like components. Only some very accurate techniques, such as NMR and EPR 13, 25, 26 , volumetric measurements 27 , Raman spectroscopy 28 , and quartz crystal microbalance with dissipation monitoring 29 have detected this phenomenon.…”

Section: Introductionmentioning

confidence: 99%

Nanoscale Membrane Domain Formation Driven by Cholesterol

Javanainen

Martinez‐Seara

Vattulainen

2017

Sci Rep

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Biological membranes generate specific functions through compartmentalized regions such as cholesterol-enriched membrane nanodomains that host selected proteins. Despite the biological significance of nanodomains, details on their structure remain elusive. They cannot be observed via microscopic experimental techniques due to their small size, yet there is also a lack of atomistic simulation models able to describe spontaneous nanodomain formation in sufficiently simple but biologically relevant complex membranes. Here we use atomistic simulations to consider a binary mixture of saturated dipalmitoylphosphatidylcholine and cholesterol — the “minimal standard” for nanodomain formation. The simulations reveal how cholesterol drives the formation of fluid cholesterol-rich nanodomains hosting hexagonally packed cholesterol-poor lipid nanoclusters, both of which show registration between the membrane leaflets. The complex nanodomain substructure forms when cholesterol positions itself in the domain boundary region. Here cholesterol can also readily flip–flop across the membrane. Most importantly, replacing cholesterol with a sterol characterized by a less asymmetric ring region impairs the emergence of nanodomains. The model considered explains a plethora of controversial experimental results and provides an excellent basis for further computational studies on nanodomains. Furthermore, the results highlight the role of cholesterol as a key player in the modulation of nanodomains for membrane protein function.

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“…Because the formation of membrane domains depends on the lipid composition and the surrounding environment (e.g. temperature) [13][14], the phase diagrams of membranes in binary and tertiary lipid systems have been systematically studied as summarized in Table 2. The membrane characteristics, which can be observed by microscopy, are herein defined as "macroscopic" properties, although these techniques can clarify the "visible" information of the membranes.…”

Section: General Characteristics Of Liposome Membranementioning

confidence: 99%

Use Liposome as a Designable Platform for Molecular Recognition ~ from “Statistical Separation” to “Recognitive Separation” ~

Umakoshi

Suga

2013

SERDJ

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A liposome membrane system attracts much interest for its use as a "designable" interface for the recognition of various biomolecules because it can provide a hydrophobic nano-environment in aqueous solution and can also induce a dynamic nature in response to the variation of its environment. The methods to characterize the membrane properties of liposome have been reviewed, focusing on the micro-phase separation of different lipids on the membrane and, also, nano-domain formation there.A possible design of the liposome membrane for the recognition of biomacromolecules was discussed based on the characterized properties, by employing the RNA molecule as a case study. Finally, a possible extension of the above-described strategy toward "recognitive separation" is discussed as a future possibility.

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Direct measurement of phase coexistence in DPPC/cholesterol vesicles using Raman spectroscopy

Cited by 43 publications

References 29 publications

Normal vibrations of ternary DOPC/DPPC/cholesterol lipid bilayers by low-frequency Raman spectroscopy

Normal vibrations of ternary DOPC/DPPC/cholesterol lipid bilayers by low-frequency Raman spectroscopy

Nanoscale Membrane Domain Formation Driven by Cholesterol

Use Liposome as a Designable Platform for Molecular Recognition ~ from “Statistical Separation” to “Recognitive Separation” ~

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