Normal vibrations of ternary DOPC/DPPC/cholesterol lipid bilayers by low-frequency Raman spectroscopy

Leonov, Dmitry V.; Dzuba, Sergei A.; Surovtsev, N. V.

doi:10.1039/c9ra06114b

Cited by 15 publications

(18 citation statements)

References 36 publications

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“…21 The latest research applied low-frequency Raman spectroscopy to show that the high cholesterol concentration induces a small interleaflet coupling. 31 Our AFM image supports the uncoupling event, so it is believed that the interleaflet coupling varies with the cholesterol concentration. Our results demonstrated that the cholesterol affects the mechanical property of the artificial bilayer and the spatial distribution of phospholipid.…”

Section: ■ Results and Discussionsupporting

confidence: 69%

“…The recent uncoupling effect of DOPC bilayers was reported by observation of a bifurcation feature in the breakthrough force . The latest research applied low-frequency Raman spectroscopy to show that the high cholesterol concentration induces a small interleaflet coupling . Our AFM image supports the uncoupling event, so it is believed that the interleaflet coupling varies with the cholesterol concentration.…”

Section: Resultssupporting

confidence: 67%

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Cholesterol Modulates the Formation of the Aβ Ion Channel in Lipid Bilayers

Gao

Lai

2020

Biochemistry

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The misfolding of amyloid beta (Aβ) is one of the predominant hallmarks in the pathology of Alzheimer’s disease (AD). In this study, we showed that the formation of the Aβ ion channel on the membrane depended on the cholesterol concentration. From a mechanical aspect, we found that cholesterol levels affected the stability and assembly of lipid bilayers. Measurements on planar lipid bilayers indicated that a small amount of cholesterol interacted with Aβ proteins and promoted the insertion process. Conversely, high cholesterol integrated the lipid bilayer and exerted an opposite effect on Aβ insertion. The Aβ ion channel was then detected by graphene-based field-effect transistors. Results demonstrated that the Aβ ion channel promoted a Ca2+ flux in the presence of 15% cholesterol but prevented a Ca2+ flux in high cholesterol. Thus, cholesterol had a complex impact on the Aβ ion channel that can be described as two different effects. First, a small amount of cholesterol interacted with Aβ and facilitated the Aβ ion channel formation in the membrane. Second, a large amount of cholesterol did not induce the ion flux in the membrane, which can be explained by the cholesterol damage to the regular distribution of the lipid bilayer. Overall, this study suggested a possible approach to consider cholesterol levels for the treatment of AD patients.

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Section: ■ Results and Discussionsupporting

confidence: 69%

Section: Resultssupporting

confidence: 67%

Cholesterol Modulates the Formation of the Aβ Ion Channel in Lipid Bilayers

Gao

Lai

2020

Biochemistry

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“…In the present work, we employ a recently developed novel experimental approachlow-frequency Raman spectroscopy. − In this approach, instead of studying atomic vibrations as is performed in conventional Raman spectroscopy, mechanical vibrations of the whole bilayer are detected. These vibrations appear between 5 and 90 cm –1 ; a narrow peak found in these experiments between 9 and 18 cm –1 (depending on the system and temperature) is attributed to an eigenmode of the normal vibrations of a lipid monolayer (i.e., of a leaflet).…”

Section: Introductionmentioning

confidence: 99%

“…In other words, this is a breathing-type mode of a single monolayer as a continuous thick plate with displacements in the normal direction. Using this approach for model biological membranes, new information on lipid–cholesterol interactions was obtained , which may appear useful for elucidating the problem of lipid raft formation (see, e.g., ref for recent references).…”

Section: Introductionmentioning

confidence: 99%

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Membrane–Sugar Interactions Probed by Low-Frequency Raman Spectroscopy: The Monolayer Adsorption Model

2020

Self Cite

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Small sugars are known to stabilize biological membranes under extreme conditions of freezing and desiccation. The proposed mechanisms of stabilization suggest membrane–sugar interactions to be either attractive or repulsive. To obtain new insight into the problem, we use a recently developed low-frequency Raman scattering approach which allows detecting membrane mechanical vibrations. For model membranes of palmitoyl-oleoyl-glycero-phosphocholine (POPC) hydrated in aqueous sucrose and trehalose solutions, we studied the Raman peak between 12 and 15 cm–1 that is attributed to an eigenmode of the normal mechanical vibrations of a lipid monolayer. For both sugars, similar results were obtained. With an increase in sugar concentration in solution, the frequency position of the peak was found to decrease by ∼13% which was interpreted as a consequence of the membrane thickening due sugar monolayer adsorption on the membrane surface. The concentration dependence of the peak frequency position was satisfactorily described by a Langmuir monolayer adsorption model. It is concluded that, at small sugar concentrations (less than 0.2 M), the membrane–sugar interactions are attractive, while at higher concentrations (more than 0.4 M) the attraction disappears. The data obtained show that one sugar molecule on the surface interacts with approximately 3–4 polar lipid heads.

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Biocompatible and optically stable hydrophobic fluorescent carbon dots for isolation and imaging of lipid rafts in model membrane

et al. 2022

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Normal vibrations of ternary DOPC/DPPC/cholesterol lipid bilayers by low-frequency Raman spectroscopy

Abstract: Upon lipid raft formation, the Raman peak corresponding to monolayer normal mechanical vibrations drops remarkably in position and width.

Cited by 15 publications

References 36 publications

Cholesterol Modulates the Formation of the Aβ Ion Channel in Lipid Bilayers

Cholesterol Modulates the Formation of the Aβ Ion Channel in Lipid Bilayers

Membrane–Sugar Interactions Probed by Low-Frequency Raman Spectroscopy: The Monolayer Adsorption Model

Biocompatible and optically stable hydrophobic fluorescent carbon dots for isolation and imaging of lipid rafts in model membrane

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