Direct measurement of doxorubicin concentration in the intact, living single cancer cell during hyperthermia

Kawai, Hideki; Minamiya, Yoshihiro; Kitamura, Michihiko; Matsuzaki, Ikuo; Hashimoto, Masaji; Suzuki, Hiroyuki; Abo, Shichisaburo

doi:10.1002/(sici)1097-0142(19970115)79:2<214::aid-cncr3>3.0.co;2-k

Cited by 37 publications

(15 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The time‐dependent cellular uptake of the loaded DOX was also seen since much stronger fluorescence was seen after 4 h than after 1 h. However, fluorescence could be detected both in the cytoplasm and nucleus for the DOX loaded micelles after both 1 h and 4 h. This is in accordance with the different cellular uptake ways. Free DOX quickly diffused through the cell membrane and concentrated in the nuclei,23 while the DOX loaded micelles were taken up via the endocytosis pathway,24 thus distribution in both cytoplasm and nucleus was observed.…”

Section: Resultsmentioning

confidence: 99%

Tunable pH‐Sensitive Poly(β‐amino ester)s Synthesized from Primary Amines and Diacrylates for Intracellular Drug Delivery

Song

Tang

et al. 2012

Macromolecular Bioscience

View full text Add to dashboard Cite

The pH sensitivity of a series of PbAEs synthesized from primary amines and diacrylates is studied. By changing alkyl groups of the amine monomers, the pKb can be tuned across a broad range (from 3.5 to 7.2). Micelles formed from a PEG-PbAE block copolymer retain the pH sensitivity of PbAE and can stably load hydrophobic molecules under neutral pH, while quickly dissociate and release their cargoes at pH ≈ 6.0. When the chemotherapy drug DOX is loaded, the micelles show efficient cell proliferation inhibition to HeLa cells and fast intracellular release. Thus, the primary-amine-based PbAEs are shown to be promising in the construction of intracellular targeting drug delivery systems.

show abstract

Section: Resultsmentioning

confidence: 99%

Tunable pH‐Sensitive Poly(β‐amino ester)s Synthesized from Primary Amines and Diacrylates for Intracellular Drug Delivery

Song

Tang

et al. 2012

Macromolecular Bioscience

View full text Add to dashboard Cite

show abstract

“…Using an elegant confocal approach, Kawai et al, demonstrated that 43 C hyperthermia increased the accumulation of doxorubicin into the nuclei of both drug-sensitive and drug-resistant oesophageal carcinoma cell lines [62]. The enhancement in uptake was 4.3 and 7.2-fold for the drug-sensitive and drug-resistant lines.…”

Section: Doxorubicinmentioning

confidence: 99%

Effects of hyperthermia in neutralising mechanisms of drug resistance in non-muscle-invasive bladder cancer

Heijden

Dewhirst

2016

International Journal of Hyperthermia

View full text Add to dashboard Cite

Non-muscle-invasive bladder cancer is a challenging disease, even given its superficial nature. It is prone to multiple recurrences and progression to muscle-invasive cancer. These features of this disease contribute significantly to reduced quality of life as well as creating significant morbidity and even mortality. Randomised trials demonstrate that when hyperthermia is added to conventional mitomycin-C treatment that local control rates and progression-free survival are substantially improved. In this review we consider how hyperthermia can exert such beneficial effects. Some of the mechanisms presented are theoretical, while others are facts. It is hoped that this review will contribute rationale for further examination of mechanisms, because an understanding of such mechanisms may lead to even better chemotherapeutic approaches, as well as potential biomarkers for predicting and monitoring treatment success.ARTICLE HISTORY

show abstract

“…For many tumour cell lines, doxorubicin is actively pumped out of cells by the multi-drug resistance membrane protein, which can be transiently damaged by heat 85,86 . Hence, transient hyperthermic damage to cellular homeostatic mechanisms that permit increased cellular or nuclear membrane permeability to doxorubicin and increased intracellular accumulation could be partly responsible 87,88 . Alternatively, RF has been shown to transiently reduce blood flow at 45-50 C 83 and cellular uptake of doxorubicin is known to increase under hypoxic conditions 89 .…”

Section: Improved Cytotoxicity Of Doxorubicinmentioning

confidence: 99%

Combination radiofrequency thermal ablation and adjuvant IV liposomal doxorubicin increases tissue coagulation and intratumoural drug accumulation

Ahmed

Goldberg

2004

International Journal of Hyperthermia

View full text Add to dashboard Cite

There has been marked interest in minimally-invasive, image-guided radiofrequency (RF) tumour ablation (i.e. coagulating tumour using short duration heating ( < 15 min) by directly applying temperatures > 50 degrees C via needle electrodes) to treat focal liver, renal, breast, bone and lung tumours. In spite of advances in RF technology and improved understanding of tumour biophysiology that now enable experimental treatment of tumours up to 5cm, investigators have been unable to achieve complete ablation in many cases, particularly at the tumour margins and adjacent to blood vessels. One strategy for overcoming these limitations has been to take advantage of complementary interactions between RF thermal ablation and chemotherapy, particularly liposomal doxorubicin preparations, to attempt more complete tumour destruction. This paper will review published laboratory investigations demonstrating that this combined treatment paradigm has the unique potential both to potentiate preferential delivery of cytotoxic agents in liposome vehicles and to maximize the completeness of ablation of a treated tumour. New confirmatory data describing increased tumour destruction with RF ablation combined with different liposome preparations, documenting increased lipid peroxidation and expanding on previously published tumour growth studies is presented. Additionally, early clinical data including a randomized, pilot clinical study on 10 patients with primary and metastatic liver tumours, in which a non-optimized combination of RF ablation and IV liposomal doxorubicin (Doxil) increased the volume of tumour destruction 25-30% compared to RF alone, will also be described in detail.

show abstract

Direct measurement of doxorubicin concentration in the intact, living single cancer cell during hyperthermia

Abstract: Hyperthermia increased the uptake of doxorubicin in the nuclei of cancer cells. Thus, the authors concluded that hyperthermia increases the cells' sensitivity to the drug.

Cited by 37 publications

References 19 publications

Tunable pH‐Sensitive Poly(β‐amino ester)s Synthesized from Primary Amines and Diacrylates for Intracellular Drug Delivery

Tunable pH‐Sensitive Poly(β‐amino ester)s Synthesized from Primary Amines and Diacrylates for Intracellular Drug Delivery

Effects of hyperthermia in neutralising mechanisms of drug resistance in non-muscle-invasive bladder cancer

Combination radiofrequency thermal ablation and adjuvant IV liposomal doxorubicin increases tissue coagulation and intratumoural drug accumulation

Contact Info

Product

Resources

About