Direct injection of vascular endothelial growth factor into the ovary of mice promotes follicular development

Quintana, Ramiro; Kopcow, L.; Sueldo, Carlos; Marconi, Guillermo; Rueda, Nidia Gomez; Barañao, Rosa Inés

doi:10.1016/j.fertnstert.2004.03.036

Cited by 34 publications

(34 citation statements)

References 27 publications

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“…Quintana et al further demonstrated that direct injection of VEGF into mouse ovaries enhanced the vascular network, promoted follicular development, and diminished apoptosis. 49 However, different isoforms of VEGF appear to have different characteristics during angiogenesis, 48,50 and there has been no long-term follow-up or functional assessment of VEGF-injected ovaries subjected to transplantation.…”

Section: Discussionmentioning

confidence: 99%

Effect of Human Endothelial Progenitor Cell (EPC)- or Mouse Vascular Endothelial Growth Factor-Derived Vessel Formation on the Survival of Vitrified/Warmed Mouse Ovarian Grafts

et al. 2014

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The aim of this study was to evaluate the effectiveness of improving angiogenesis at graft sites on the survival of follicles in transplanted ovarian tissue. Matrigel containing 5 Â 10 5 of cord blood-derived endothelial progenitor cells (EPCs) or 200 ng of mouse vascular endothelial growth factor (VEGF) was injected subcutaneously into BALB/c-Nu mice. After 1 week, vitrified/ warmed ovaries from female B6D2F1 mice were subcutaneously transplanted into the injection sites. After 1, 2, and 4 weeks posttransplantation, the ovaries were recovered and subjected to histological analysis. Oocytes were collected from the transplanted ovaries, and their fertilization, embryonic development, and delivery were also observed. Vitrified/warmed ovaries transplanted into EPC-or VEGF-treated sites developed more blood vessels and showed better follicle survival than those transplanted into sham-injected sites. Normal embryonic development and consequent live births were obtained using oocytes recovered from cryopreserved/transplanted ovaries. Treatment with EPCs or VEGF could prevent the ischemic damage during the early revascularization stage of ovarian transplantation.

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Section: Discussionmentioning

confidence: 99%

Effect of Human Endothelial Progenitor Cell (EPC)- or Mouse Vascular Endothelial Growth Factor-Derived Vessel Formation on the Survival of Vitrified/Warmed Mouse Ovarian Grafts

et al. 2014

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“…Conversely, many studies have indicated that exogenous injection of VEGF promoted follicular development in pigs (Shimizu et al 2003), mice (Quintana et al 2004), and rats (Danforth et al 2003). These studies indicate that VEGF is one of the crucial factors for follicular development in mammals.…”

Section: Introductionmentioning

confidence: 99%

“…In rodents, although exogenous administration of VEGF stimulates follicular development (Danforth et al 2003, Quintana et al 2004, Iijima et al 2005, it is unknown whether the follicles generated as a result of VEGF stimulation ovulate or not. Therefore, to examine whether a large number of preovulatory follicles are produced as a result of injection of VEGF gene fragments directly into the rat ovary and whether these follicles ovulate, we treated immature female rats with a combination of hormones and VEGF gene.…”

Section: Introductionmentioning

confidence: 99%

Effect of direct ovarian injection of vascular endothelial growth factor gene fragments on follicular development in immature female rats

Shimizu¹,

Izutsu²,

Miyabayashi³

et al. 2007

Reproduction

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Vascular endothelial growth factor (VEGF) expression in granulosa cells is associated with the thecal vasculature growth during ovarian follicular development. We hypothesized that injection of VEGF gene fragments directly into the rat ovary would induce production of a large number of ovulatory follicles and that these follicles would ovulate. To test this hypothesis, we treated immature female rats with combinations of hormones and VEGF gene fragments. The animals were divided into two groups: one group received solution containing transfection reagents as a control (nZ5), while the other group received direct ovarian injection of VEGF gene fragments at 19 (nZ5), 21 (nZ5), 23 (nZ5), or 25 (nZ5) days after birth followed by i.p. administration of 20 IU equine chorionic gonadotropin (eCG) at the age of 26 days. Forty-eight hours after eCG injection, animals were given 20 IU human chorionic gonadotropin (hCG) i.p. and then the oocytes in both groups were counted. The maximum number of ovulated oocytes was obtained when the VEGF gene fragments were injected into the rat ovary at 21 days after birth. Histological examination revealed that the injection of VEGF gene fragments markedly increased the vascular density around the preovulatory follicles and also the number of these follicles. Our data provide the first reported evidence that most ovulatory follicles generated by injection of VEGF gene fragments are able to ovulate upon hCG treatment. These results demonstrate that injection of VEGF gene fragments directly into the ovary stimulates the development of antral follicles by inducing the formation of thecal vasculature in immature female rats.

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“…VEGF 120 and VEGF 164 are expressed in the mammalian ovary [7,8] and are associated with follicular angiogenesis during follicular development [9,10]. In vivo injection of VEGF gene or protein induces the emergence of a large number of preovulatory and antral follicles [11][12][13]. These reports indicate that VEGF is a crucial factor during follicular development in the ovary.…”

mentioning

confidence: 99%

Differential Effect of Follicle-Stimulating Hormone and Estradiol on Expressions of Vascular Endothelial Growth Factor (VEGF) 120, VEGF164 and Their Receptors in Bovine Granulosa Cells

Shimizu

Jayawardana

Tetsuka

et al. 2007

Journal of Reproduction and Development

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Abstract. Vascular endothelial growth factor (VEGF) isoforms (VEGF 120 and VEGF 164) secreted by granulosa cells are involved in thecal angiogenesis during follicular development in the bovine ovary. However, whether the transcript of the VEGF120 and VEGF164 isoforms differs during follicular development in the ovary is still unknown. We first examined the gene expression of VEGF120, VEGF164, fms-like tyrosine kinase (Flt-1), and fetal liver kinase (Flk-1) in the granulosa cells (GCs) and theca cells (TCs) of pre-selection and post-selection follicles (PRF and POF respectively) from the bovine ovary. Then we examined the effects of FSH and estradiol (E2) on these factors in cultured bovine GCs. Messenger RNA (mRNA) expression was quantified using real-time PCR methods. The concentrations of E2 and P4 in the follicular fluid (FF) of the PRF and POF were estimated using an enzyme immunoassay (EIA). The concentrations of E2 and P4 in the FF were significantly higher in the POF than in the PRF. The ratio of E2/P4 in PRF and POF was 0.37 and 3.8, respectively. The expression levels of the VEGF120, VEGF164, and Flk-1 mRNAs in the GCs of POF with high E2 concentration were higher than those of PRF. The levels of the Flt-1 and Flk-1 mRNAs in the TCs were not different between PRF and POF. Since E2 in the FF of the POF used in the present study was high compared with the PRF, we examined the effects of E2 and FSH on the expression of the above genes using cultured GCs. Expression of VEGF120 mRNA was induced by a low concentration (1 ng/ml) of E2, whereas the levels of VEGF164 and Flk-1 mRNAs were not affected by E2. FSH stimulated the expression of the VEGF isoforms and Flk-1 genes. Moreover, the expression of those genes was enhanced when low E2 (1 ng/ml) was added to FSH. In conclusion, our data indicates that the VEGF isoforms have a follicle stage-dependent expression pattern. Thus, our results suggest that the expression of VEGF isoforms may be associated with characterization of the preovulatory phenotype during follicle development in the bovine ovary.

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Direct injection of vascular endothelial growth factor into the ovary of mice promotes follicular development

Cited by 34 publications

References 27 publications

Effect of Human Endothelial Progenitor Cell (EPC)- or Mouse Vascular Endothelial Growth Factor-Derived Vessel Formation on the Survival of Vitrified/Warmed Mouse Ovarian Grafts

Effect of Human Endothelial Progenitor Cell (EPC)- or Mouse Vascular Endothelial Growth Factor-Derived Vessel Formation on the Survival of Vitrified/Warmed Mouse Ovarian Grafts

Effect of direct ovarian injection of vascular endothelial growth factor gene fragments on follicular development in immature female rats

Differential Effect of Follicle-Stimulating Hormone and Estradiol on Expressions of Vascular Endothelial Growth Factor (VEGF) 120, VEGF164 and Their Receptors in Bovine Granulosa Cells

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