Direct injection of large volumes of plasma/serum on a new biocompatible extraction column for the determination of atenolol, propranolol and ibuprofen

Restricted access materials and large particle supports for on-line sample preparation: an attractive approach for biological fluids analysis

“…Protein-coated silica material has exhibited good performances for the analysis of pharmaceutical compounds in biological matrices [31,107,108]. BioTrap can tolerate up to 30 ml of biological fluids without notable performance deterioration [109]. Nowadays, the BioTrap support is available with a hydrophobic polymer (BioTrap MS) and presents the advantage of being used in a wider pH range (from 2 to 10) than a silica material (pH range from 2.5 to 7.5).…”

Methylcellulose-immobilized Reversed-phase Precolumn for Direct Analysis of Drugs in Plasma by HPLC

“…As a protein-immobilized stationary phase capable of plasma direct injection, octadecylsilane (ODS) treated with human plasma was developed and plasma direct injection capability was shown by Yoshida et al 12,13 α1-Acidglycoprotein (α1-AGP)-, ovomucoid-and avidin-coated columns had been developed, [14][15][16] and are capable of plasma direct injection. [17][18][19][20] Furthermore, direct analysis of drugs in serum using zwitterionic bile acid derivative-coated ODS was reported by Umemura et al 21 Several polymer-modified reversed-phase column materials such as the shielded hydrophobic phase (SHP) and the semipermeable surface (SPS) had also been reported. 8,[22][23][24] SHP consists of a hydrophilic, polymeric, water-solvated phase, covalently bonded to a silica bed.…”

Role of biological matrices during the analysis of chiral drugs by liquid chromatography

“…The different types of RAM have been classified and reviewed in some publications [137][138][139][140]. Beside size exclusion, which plays the dominant role in the restricted retention of proteins, irreversible protein binding and accumulation is prevented by the generation of a protective, non-adsorptive, hydrophilic outer packing surface by a protein coating [141][142][143], polymer coating [138,144,145], or enzyme-catalyzed hydrolysis [146].…”