Direct injection method for HPLC/MS/MS analysis of acrylamide in aqueous solutions: application to adsorption experiments

Abstract

Polyacrylamides are polymers used in many fields and represent the main source of release of the highly toxic acrylamide in the environment. In this work, a simple, rapid, and sensitive analytical method was developed with HPLC/MS/MS and direct injection for acrylamide analysis in water and adsorption samples. AFNOR standards NF T90-210 and NF T90-220 were used for the analytical method validation and uncertainty estimation. Limit of quantification (LOQ) for acrylamide was 1 μg/L, and accuracy was checked at three acrylamide levels (1, 6, and 10 μg/L). Uncertainties were estimated at 34.2, 22, and 12.4 % for acrylamide concentrations at LOQ, 6 μg/L, and 10 μg/L, respectively. Acrylamide adsorption on clays (kaolinite, illite) and sludge was then studied as a function of pH, time, and acrylamide concentrations. Acrylamide adsorption on kaolinite, illite, and sludge was found to be very weak since adsorption percentages were inferior to 10 %, whatever the pH value and the initial acrylamide concentration. The low affinity of acrylamide for clays and sludge is likely due to its hydrophilic property, small size, and charge neutrality.

Superabsorbent polymers in soil: The new microplastics?

“…Besides considerably lower detection limits, an MS is inherently more specific and less prone to false-positive detections compared to UV or RI detectors. Detection limits as low as 2 ng L À1 are common to liquid chromatography mass spectrometry applications, which are, however, mostly used for the quantification of SAP monomers like acrylamide in mushrooms (Castle, 1993) or drinking water (Backe et al, 2014;Mnif et al, 2014). Similarly, GC/MS may be applied for the analysis of PEG oligomers (Onigbinde et al, 2001).…”

An indirect competitive enzyme-linked immunosorbent assay for acrylamide detection based on a monoclonal antibody

“…To date, numerous instrumental analytical methods have been used to detect AA residues in food samples due to their sensitivity and selectivity. Reports on liquid chromatography-mass spectrometry (LC-MS) (Lee et al, 2015), LC-MS/MS (Alpozen, Guven, Ozdestan, & Uren, 2015), gas chromatography-mass spectrometry (GC-MS) (Molina-Garcia et al, 2015;Negoita et al, 2015),high-performance liquid chromatography (HPLC) (Xu, Zhang, Qiao, Xu, & Song, 2012;Zhang et al, 2015), HPLC-MS/MS (Mnif, Hurel, & Marmier, 2015;Zhao, Li, Li, Qiao, & Xu, 2015) and HPLC-UV (Oroian et al, 2015) have been published in recent years. These methods are precise and effective, but are time-consuming and expensive.…”