Direct identification of chlamydiae from clinical samples using a DNA microarray assay—A validation study

Borel, Nicole; Kempf, Evelyne; Hotzel, Helmut; Schubert, Evelyn; Torgerson, Paul R.; Slickers, Peter; Ehricht, Ralf; Tasara, Taurai; Pospischil, Andreas; Sachse, Konrad

doi:10.1016/j.mcp.2007.06.003

Cited by 91 publications

(77 citation statements)

References 22 publications

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“…However, complementary MLST, 16S, and 23S rRNA gene analysis showed that all northern gannet samples are related to the well-known cluster of C. psittaci and C. abortus species, but in a separate subcluster. Examination of these samples with the DNA microarray for Chlamydiaceae (57) revealed no reaction with species-specific probes, thus suggesting that these samples do not belong to any of the established chlamydial species (data not shown). For C. psittaci detection, both ompA-and incA-based qPCR systems were used in the present study.…”

Section: Discussionmentioning

confidence: 98%

Chlamydiaceae in North Atlantic Seabirds Admitted to a Wildlife Rescue Center in Western France

Aaziz

Gourlay

Vorimore

et al. 2015

Appl Environ Microbiol

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f Birds are the primary hosts of Chlamydia psittaci, a bacterium that can cause avian chlamydiosis in birds and psittacosis in humans. Wild seabirds are frequently admitted to wildlife rescue centers (WRC) at European Atlantic coasts, for example, in connection with oil spills. To investigate the extent of chlamydial shedding by these birds and the resulting risk for animals in care and the medical staff, seabirds from a French WRC were sampled from May 2011 to January 2014. By use of a quantitative PCR (qPCR), 195 seabirds belonging to 4 orders, 5 families and 13 species were examined, of which 18.5% proved to be Chlamydiaceae positive. The highest prevalence of shedders was found in northern gannets (Morus bassanus) (41%), followed by European herring gulls (Larus argentatus) (14%) and common murres (Uria aalge) (7%). Molecular characterization and phylogenetic analysis of qPCR-positive northern gannet samples revealed two variants of a strain closely related to C. psittaci. In European herring gulls and in one common murre, strains showing high sequence similarity to the atypical Chlamydiaceae-like C122 previously found in gulls were detected. Our study shows that seabirds from the northeastern Atlantic Ocean carry several chlamydial organisms, including C. psittaci-related strains. The staff in WRCs should take protective measures, particularly in the case of mass admissions of seabirds.

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Section: Discussionmentioning

confidence: 98%

Chlamydiaceae in North Atlantic Seabirds Admitted to a Wildlife Rescue Center in Western France

Aaziz

Gourlay

Vorimore

et al. 2015

Appl Environ Microbiol

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“…The samples with at least one positive Ct value were examined using the speciesspecific 23S ArrayTube (AT) microarray assay as described by Borel et al (2008).…”

Section: Arraytube Microarray Identification Of Chlamydial Speciesmentioning

confidence: 99%

Alpine ibex (Capra i. ibex) is not a reservoir for chlamydial infections of domestic ruminants and humans

et al. 2010

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Chlamydophila (C.) abortus is the most common infectious abortigenic agent in small domestic ruminants in Switzerland. In contrast, the knowledge about chlamydiae in wild ruminants is scarce. As interactions between livestock and Alpine ibex (Capra i. ibex) occur on alpine pastures, the question raises if wild ruminants could play a role as carriers of chlamydiae. Thus, we investigated the prevalence of chlamydiae in Alpine ibex in Switzerland. In total, 624 sera, 676 eye swabs, 84 organ samples and 51 faecal samples from 664 ibex were investigated. Serum samples were tested by two commercial ELISA kits specific for C. abortus. Eye swabs, organs and faecal samples were examined by a Chlamydiaceae-specific real-time PCR. Positive cases were further investigated by the ArrayTube (AT) microarray method for chlamydial species determination. Of 624 serum samples investigated, 612 animals were negative, whereas nine sera (1.5%) reacted positively in one of the two tests and three sera showed an inconclusive result. Eye swabs of seven out of 412 ibex (1.7%) were tested positive for Chlamydiaceae by real-time PCR. By AT microarray, Chlamydophila pecorum was identified in two animals, Chlamydophila pneumoniae was detected in one animal and a mixed infection with Chlamydophila abortus and Chlamydophila pecorum was found in four animals. Organs and faecal samples were all negative by real-time PCR analysis. In summary, we conclude that C. abortus is not a common infectious agent in the Swiss ibex population. To our knowledge, this is the first description of C. pneumoniae in ibex. Further studies are necessary to elucidate the situation in other species of wild ruminants as chamois (Rupicapra r. rupicapra), red deer (Cervus elaphus) and roe deer (Capreolus c. capreolus) in Switzerland. (C.) abortus is the most common infectious abortigenic agent in small domestic ruminants in Switzerland. In contrast, the knowledge about chlamydiae in wild ruminants is scarce. As interactions between livestock and Alpine ibex (Capra i. ibex) occur on alpine pastures, the question raises if wild ruminants could play a role as carriers of chlamydiae. Thus, we investigated the prevalence of chlamydiae in Alpine ibex in Switzerland. In total, 624 sera, 676 eye swabs, 84 organ samples and 51 faecal samples from 664 ibex were investigated. Serum samples were tested by two commercial ELISA kits specific for C. abortus. Eye swabs, organs and faecal samples were examined by a Chlamydiaceae-specific real-time PCR. Positive cases were further investigated by the ArrayTube (AT) microarray method for chlamydial species determination. Of 624 serum samples investigated, 612 animals were negative, whereas nine sera (1.5%) reacted positively in one of the two tests and three sera showed an inconclusive result. Eye swabs of seven out of 412 ibex (1.7%) were tested positive for Chlamydiaceae by real-time PCR. By AT microarray, Chlamydophila pecorum was identified in two animals, Chlamydophila pneumoniae was detected in one animal and a mixed infec...

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“…All positive and questionable samples were further investigated by ArrayTube Microarray (AT) (Alere) as described previously (Borel et al, 2008). Samples positive by real-time PCR for Chlamydiaceae but negative by AT were investigated by a PCR method amplifying a 298-bp product of the 16S rRNA gene specific for the order Chlamydiales (Everett et al, 1999).…”

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confidence: 99%

Deer as a potential wildlife reservoir for Parachlamydia species

Stokar-Regenscheit¹,

Holzwarth²,

Greub³

et al. 2012

The Veterinary Journal

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Wildlife populations represent an important reservoir for emerging pathogens and transboundary livestock diseases. However, detailed information relating to the occurrence of endemic pathogens such as those of the order Chlamydiales in such populations is lacking. During the hunting season of 2008, 863 samples (including blood, conjunctival swabs, internal organs and faeces) were collected in the Eastern Swiss Alps from 99 free-living red deer (Cervus elaphus) and 64 free-living roe deer (Capreolus capreolus) and tested using ELISA, PCR and immunohistochemistry for members of the family Chlamydiaceae and the genus Parachlamydia. Parachlamydia spp. were detected in the conjunctival swabs, faeces and internal organs of both species of deer (2.4% positive, with a further 29.5% inconclusive). The very low occurrence of Chlamydiaceae (2.5%) was in line with serological data (0.7% seroprevalence for Chlamydia abortus). Further investigations are required to elucidate the zoonotic potential, pathogenicity, and distribution of Parachlamydia spp. in wild ruminants.

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Direct identification of chlamydiae from clinical samples using a DNA microarray assay—A validation study

Cited by 91 publications

References 22 publications

Chlamydiaceae in North Atlantic Seabirds Admitted to a Wildlife Rescue Center in Western France

Chlamydiaceae in North Atlantic Seabirds Admitted to a Wildlife Rescue Center in Western France

Alpine ibex (Capra i. ibex) is not a reservoir for chlamydial infections of domestic ruminants and humans

Deer as a potential wildlife reservoir for Parachlamydia species

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