Chlamydiaceae in North Atlantic Seabirds Admitted to a Wildlife Rescue Center in Western France

Aaziz, Rachid; Gourlay, Philippe; Vorimore, F.; Sachse, Konrad; Siarkou, Victoria I.; Laroucau, Karine

doi:10.1128/aem.00778-15

Cited by 36 publications

(28 citation statements)

References 58 publications

(90 reference statements)

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“…Rhabdochlamydia porcellionis," first detected in terrestrial isopods (25,26), and "Ca. Rhabdochlamydia crassificans," found in the cockroach Blatta orientalis (27) Since then, rhabdochlamydial DNA has also been detected in dwarf spiders (28), sea birds (29), and ticks (14,24) but also in human respiratory samples (11,17,30). However, it is unknown how Rhabdochlamydiaceae could be transmitted between animal species and to humans, but ticks could serve as vectors (23).…”

Section: Discussionmentioning

confidence: 99%

Molecular Evidence of Chlamydia-Like Organisms in the Feces of Myotis daubentonii Bats

Hokynar

Vesterinen

Lilley

et al. 2017

Appl Environ Microbiol

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Chlamydia-like organisms (CLOs) are recently identified members of the Chlamydiales order. CLOs share intracellular lifestyles and biphasic developmental cycles, and they have been detected in environmental samples as well as in various hosts such as amoebae and arthropods. In this study, we screened bat feces for the presence of CLOs by molecular analysis. Using pan-Chlamydiales PCR targeting the 16S rRNA gene, Chlamydiales DNA was detected in 54% of the specimens. PCR amplification, sequencing, and phylogenetic analysis of the 16S rRNA and 23S rRNA genes were used to classify positive specimens and infer their phylogenetic relationships. Most sequences matched best with Rhabdochlamydia species or uncultured Chlamydia sequences identified in ticks. Another set of sequences matched best with sequences of the Chlamydia genus or uncultured Chlamydiales from snakes. To gain evidence of whether CLOs in bat feces are merely diet borne, we analyzed insects trapped from the same location where the bats foraged. Interestingly, the CLO sequences resembling Rhabdochlamydia spp. were detected in insect material as well, but the other set of CLO sequences was not, suggesting that this set might not originate from prey. Thus, bats represent another potential host for Chlamydiales and could harbor novel, previously unidentified members of this order. IMPORTANCE Several pathogenic viruses are known to colonize bats, and recent analyses indicate that bats are also reservoir hosts for bacterial genera. Chlamydialike organisms (CLOs) have been detected in several animal species. CLOs have high 16S rRNA sequence similarity to Chlamydiaceae and exhibit similar intracellular lifestyles and biphasic developmental cycles. Our study describes the frequent occurrence of CLO DNA in bat feces, suggesting an expanding host species spectrum for the Chlamydiales. As bats can acquire various infectious agents through their diet, prey insects were also studied. We identified CLO sequences in bats that matched best with sequences in prey insects but also CLO sequences not detected in prey insects. This suggests that a portion of CLO DNA present in bat feces is not prey borne. Furthermore, some sequences from bat droppings not originating from their diet might well represent novel, previously unidentified members of the Chlamydiales order.

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Section: Discussionmentioning

confidence: 99%

Molecular Evidence of Chlamydia-Like Organisms in the Feces of Myotis daubentonii Bats

Hokynar

Vesterinen

Lilley

et al. 2017

Appl Environ Microbiol

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“…However, the majority of samples from this study was not assigned to any of the established genotypes, but formed two separate branches designated G1 and G2 in the omp A sequence-based dendrogram ( Fig 2 ). Although genotyping in Chlamydiaceae relies upon omp A, the gene encoding the major protein antigen of chlamydiae, this target is unsuitable for reconstruction of phylogenetic relationships and classification, due to its polymorphic character caused by high mutation rates and frequent inter-strain recombination events [8, 38]. Instead, MLST as well as 16S rRNA, IGS and partial 23S rRNA gene analyses of representative isolates of 1V, G1 and G2 were employed to elucidate their phylogenetic position.…”

Section: Discussionmentioning

confidence: 99%

“…Representatives of Psittaciformes and Columbiformes are the most prominent hosts for chlamydiae [5], but prevalence studies revealed their occurrence, for example, also in Anseriformes, Charadriiformes, Passeriformes, Falconiformes, Accipitriformes or Procellariformes [8, 16–20]. A recent survey in wildfowl from Poland focusing on wetland birds [21] found an overall prevalence of 7.4% with C .…”

Section: Introductionmentioning

confidence: 99%

“…psittaci as the predominant chlamydial agent in cormorants and mallards. Interestingly, many studies report the detection of atypical or non-typable Chlamydiaceae in wild birds [8–9, 16, 21], however, their further characterization was often hampered by unsuccessful cultivation attempts and, thus, the lack of isolates from the unknown species or genotypes. Therefore, the aim of our study was not solely the collection of prevalence data for Chlamydiaceae in wild birds in ten out of sixteen Polish districts (voivodeships), but also the isolation of chlamydial agents to enable their in-depth molecular characterization as well as further investigations into epidemiology, host preference, pathogenicity and zoonotic potential.…”

Section: Introductionmentioning

confidence: 99%

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Dissemination and genetic diversity of chlamydial agents in Polish wildfowl: Isolation and molecular characterisation of avian Chlamydia abortus strains

et al. 2017

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Wild birds are considered as a reservoir for avian chlamydiosis posing a potential infectious threat to domestic poultry and humans. Analysis of 894 cloacal or fecal swabs from free-living birds in Poland revealed an overall Chlamydiaceae prevalence of 14.8% (n = 132) with the highest prevalence noted in Anatidae (19.7%) and Corvidae (13.4%). Further testing conducted with species-specific real-time PCR showed that 65 samples (49.2%) were positive for C. psittaci whereas only one was positive for C. avium. To classify the non-identified chlamydial agents and to genotype the C. psittaci and C. avium-positive samples, specimens were subjected to ompA-PCR and sequencing (n = 83). The ompA-based NJ dendrogram revealed that only 23 out of 83 sequences were assigned to C. psittaci, in particular to four clades representing the previously described C. psittaci genotypes B, C, Mat116 and 1V. Whereas the 59 remaining sequences were assigned to two new clades named G1 and G2, each one including sequences recently obtained from chlamydiae detected in Swedish wetland birds. G1 (18 samples from Anatidae and Rallidae) grouped closely together with genotype 1V and in relative proximity to several C. abortus isolates, and G2 (41 samples from Anatidae and Corvidae) grouped closely to C. psittaci strains of the classical ABE cluster, Matt116 and M56. Finally, deep molecular analysis of four representative isolates of genotypes 1V, G1 and G2 based on 16S rRNA, IGS and partial 23S rRNA sequences as well as MLST clearly classify these isolates within the C. abortus species. Consequently, we propose an expansion of the C. abortus species to include not only the classical isolates of mammalian origin, but also avian isolates so far referred to as atypical C. psittaci or C. psittaci/C. abortus intermediates.

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“…Aaziz et al (2015);; Herrmann et al (2006); Jencek et al (2012); Ladds (2009); Miller and Fowler (2015); Mykytowycz et al (1955); Thomas et al…”

mentioning

confidence: 99%

Sphenisciformes, Gaviiformes, Podicipediformes, Procellariiformes, and Pelecaniformes

Stidworthy

Denk

2018

Pathology of Wildlife and Zoo Animals

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Chlamydiaceae in North Atlantic Seabirds Admitted to a Wildlife Rescue Center in Western France

Cited by 36 publications

References 58 publications

Molecular Evidence of Chlamydia-Like Organisms in the Feces of Myotis daubentonii Bats

Molecular Evidence of Chlamydia-Like Organisms in the Feces of Myotis daubentonii Bats

Dissemination and genetic diversity of chlamydial agents in Polish wildfowl: Isolation and molecular characterisation of avian Chlamydia abortus strains

Sphenisciformes, Gaviiformes, Podicipediformes, Procellariiformes, and Pelecaniformes

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