Direct Genetic Analysis of Ten Cancer Cells: Tuning Sensor Structure and Molecular Probe Design for Efficient mRNA Capture

Vasilyeva, Elizaveta; Lam, Brian; Fang, Zhichao; Minden, Mark D.; Sargent, Edward H.

doi:10.1002/ange.201006793

Cited by 8 publications

(6 citation statements)

References 22 publications

(22 reference statements)

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“…Electrochemical nucleic acid analysis carried out using NMEs has also been applied to the quantitation of a range of analytes including small molecules and proteins 40 , and biomarkers including cancer-related gene fusions 41,42 and microRNAs 43 . In view of its low limit of detection, the strategy also shows promise in the analysis of gene expression in rare CTCs isolated from the bloodstreams of patients with cancer 44 .…”

Section: Nucleic Acid Detection With Record-breaking Speedmentioning

confidence: 99%

Advancing the speed, sensitivity and accuracy of biomolecular detection using multi-length-scale engineering

et al. 2014

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Rapid progress in identifying disease biomarkers has increased the importance of creating high-performance detection technologies. Over the last decade, the design of many detection platforms has focused on either the nano or micro length scale. Here, we review recent strategies that combine nano- and microscale materials and devices to produce large improvements in detection sensitivity, speed and accuracy, allowing previously undetectable biomarkers to be identified in clinical samples. Microsensors that incorporate nanoscale features can now rapidly detect disease-related nucleic acids expressed in patient samples. New microdevices that separate large clinical samples into nanocompartments allow precise quantitation of analytes, and microfluidic systems that utilize nanoscale binding events can detect rare cancer cells in the bloodstream more accurately than before. These advances will lead to faster and more reliable clinical diagnostic devices.

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Section: Nucleic Acid Detection With Record-breaking Speedmentioning

confidence: 99%

Advancing the speed, sensitivity and accuracy of biomolecular detection using multi-length-scale engineering

et al. 2014

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“…A controlled nanostructure can be generated by employing an appropriate electrolyte and voltage. Kelley’s group prepared numerous of nanostructured electrodes and demonstrated their applications in detection of nucleic acid [ 67 , 68 , 69 , 70 ]. For example, in 2009, Yang et al [ 67 ] prepared a nanostructured electrode for miRNAs detection by plating gold on the silicon surface ( Figure 9 ).…”

Section: Nanomaterials-based Electrochemical Strategies For Mirnasmentioning

confidence: 99%

Nanomaterials-Based Sensing Strategies for Electrochemical Detection of MicroRNAs

Xia

Zhang

2014

Materials

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MicroRNAs (miRNAs) play important functions in post-transcriptional regulation of gene expression. They have been regarded as reliable molecular biomarkers for many diseases including cancer. However, the content of miRNAs in cells can be low down to a few molecules per cell. Thus, highly sensitive analytical methods for miRNAs detection are desired. Recently, electrochemical biosensors have held great promise as devices suitable for point-of-care diagnostics and multiplexed platforms for fast, simple and low-cost nucleic acid analysis. Signal amplification by nanomaterials is one of the most popular strategies for developing ultrasensitive assay methods. This review surveys the latest achievements in the use of nanomaterials to detect miRNAs with a focus on electrochemical techniques.

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“…The long length of the 100 μm sensor resulted in its extension into the solution, which allowed it to interact with the target molecules; this decreased the slow travel of the target molecules through the solution to the fixed sensors and thus increased the detection rate. By using the same platform, the authors also successfully detected mRNA derived from as few as ten K562 cells (chronic myeloid leukemia cells) in unpurified and unprocessed lysate and in the presence of a 5 000 000‐fold excess of blood cells 68. This fractal nanostructure based platform is label‐free and does not require the sample to be processed in any way; thus, it shows great promise for clinical use.…”

Section: Advanced Application In Biomedical Applicationsmentioning

confidence: 99%

Designing Fractal Nanostructured Biointerfaces for Biomedical Applications

Zhang

Wang

2014

ChemPhysChem

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Fractal structures in nature offer a unique "fractal contact mode" that guarantees the efficient working of an organism with an optimized style. Fractal nanostructured biointerfaces have shown great potential for the ultrasensitive detection of disease-relevant biomarkers from small biomolecules on the nanoscale to cancer cells on the microscale. This review will present the advantages of fractal nanostructures, the basic concept of designing fractal nanostructured biointerfaces, and their biomedical applications for the ultrasensitive detection of various disease-relevant biomarkers, such microRNA, cancer antigen 125, and breast cancer cells, from unpurified cell lysates and the blood of patients.

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Direct Genetic Analysis of Ten Cancer Cells: Tuning Sensor Structure and Molecular Probe Design for Efficient mRNA Capture

Cited by 8 publications

References 22 publications

Advancing the speed, sensitivity and accuracy of biomolecular detection using multi-length-scale engineering

Advancing the speed, sensitivity and accuracy of biomolecular detection using multi-length-scale engineering

Nanomaterials-Based Sensing Strategies for Electrochemical Detection of MicroRNAs

Designing Fractal Nanostructured Biointerfaces for Biomedical Applications

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