Direct evidence of oxidative damage in acute and chronic phases of experimental colitis in rats

Loguercio, C.; D’Argenio, Giuseppe; Cave, M. Delle; Cosenza, V.; Valle, Nicola Della; Mazzacca, G; Blanco, C. Del Vecchio

doi:10.1007/bf02088238

Cited by 77 publications

(47 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…So increased oxidative stress and impairment of antioxidant defences might contribute to the pathogenesis of IBD. The present study results with the TNBS-induced lipid peroxidation are similar to a study conducted in the recent past (Loquercio et al, 1996). This study showed the oxidative/ antioxidative status in the colon after TNBS administration causes a significant decrease in the antioxidant enzyme activities and increased lipid peroxidation.…”

Section: Discussionmentioning

confidence: 38%

Effect of different doses of Manuka honey in experimentally induced inflammatory bowel disease in rats

Prakash

Medhi

Avti

et al. 2008

Phytotherapy Research

View full text Add to dashboard Cite

To evaluate the effect of different doses of Manuka honey in experimentally induced inflammatory bowel disease in rats. Adult Wistar rats of either sex were used (n = 30). Colitis was induced by a single intracolonic administration of TNBS dissolved in 35% ethanol. The rats (n = 30) were divided into five groups (n = 6) and were treated with vehicle (ethanol), TNBS, Manuka honey (5 g/kg, p.o.), Manuka honey (10 g/kg, p.o.) or sulfasalazine (360 mg/kg, p.o.) body weight for 14 days. After completion of treatment, the animals were killed and the following parameters were assessed: morphological score, histological score and different antioxidant parameters.Manuka honey at different doses provided protection against TNBS-induced colonic damage. There was significant protection with Manuka honey 5 g/kg as well as with 10 g/kg body weight compared with the control (p < 0.001). All the treated groups showed reduced colonic inflammation and all the biochemical parameters were significantly reduced compared with the control in the Manuka honey treated groups (p < 0.001). Manuka honey at different doses restored lipid peroxidation as well as improved antioxidant parameters. Morphological and histological scores were significantly reduced in the low dose Manuka honey treated group (p < 0.001). In the inflammatory model of colitis, oral administration of Manuka honey 5 g/kg and Manuka honey 10 g/kg body weight significantly reduced the colonic inflammation. The present study indicates that Manuka honey is efficacious in the TNBS-induced rat colitis model, but these results require further confirmation in human studies.

show abstract

Section: Discussionmentioning

confidence: 38%

Effect of different doses of Manuka honey in experimentally induced inflammatory bowel disease in rats

Prakash

Medhi

Avti

et al. 2008

Phytotherapy Research

View full text Add to dashboard Cite

show abstract

“…Glutathione is considered an important cellular scavenger of free radicals, both by its direct antioxidant ability and by its property to be a substrate for the action of glutathione peroxidases and glutathione transferases [33]. Decreased glutathione levels, which are indicative of oxidant stress, have been detected in human [34] and experimental [7,8,31] colonic inflammation. Because morin has been described as an antioxidative and scavenger agent [5,9,11], the colonic MDA content and glutathione levels, as markers of intestinal oxidative status, were measured to verify whether this mechanisms was involved in the anti-inflammatory effect of morin.…”

Section: Discussionmentioning

confidence: 44%

“…Recently, Loguercio et al [31] proposed that TNBS/ethanol initially causes damage by production of free radical species, without the mediation of products derived from inflammatory processes. The initially enhanced production of free radicals induces lipoperoxidation which results in the release of chemoattractant mediators for inflammatory cells like neutrophils, which infiltrate the damaged mucosa.…”

Section: Discussionmentioning

confidence: 44%

Effects of Morin on an Experimental Model of Acute Colitis in Rats

Ma¹,

Gálvez²,

Me³

et al. 1998

Pharmacology

View full text Add to dashboard Cite

The flavonoid morin was tested for anti-inflammatory activity in trinitrobenzenesulfonic acid (TNBS)-induced rat colitis. Rats were pretreated orally with several doses of the flavonoid (5, 10, 25, 100 and 200 mg/kg) 48, 24 and 1 h before and 24 h after colitis induction and examined for colonic damage 48 h after colitis induction. Colonic inflammation was characterized by diffuse hemorrhagic necrosis of the mucosa, bowel wall thickening, impairment of fluid absorption, increase in myeloperoxidase (MPO) activity, enhanced leukotriene B₄ (LTB₄) synthesis, glutathione depletion and increased levels of malonyldialdehyde (MDA). Morin treatment, at doses ranging from 10 to 200 mg/kg, significantly reduced colonic macroscopic damage. This beneficial effect was also confirmed by inhibition of colonic MPO activity. Several mechanisms may contribute to the protective effect exerted by morin. First, inhibition of colonic LTB₄ synthesis is a common feature for all the active doses of the flavonoid. Second, the antioxidant properties of morin, which partially prevented colonic glutathione depletion (at doses of 10 and 25 mg/kg) or inhibited colonic MDA production (at doses of 100 and 200 mg/kg), can collaborate in preventing TNBS-induced inflammation.

show abstract

“…it is a flavonoid glycoside which is isolated from citrus plant [1]. Hesperidin has been proven to have anti-inflammation, antimicrobial, antioxidant, anti-hemorrhoid, and anticancer activity so it could be used in therapy of some colon related diseases such as hemorrhoid, chronic vein insufficiency, colon cancer, and ulcerative colitis [1][2][3][4][5][6], however, hesperidin has low solubility on digestive tract (<100 mg/l) as well as low bioavailability (<25%) [1,7]. Therefore, the correct delivery system is required to increase the bioavailability and therapy effectiveness from hesperidin, one of them is by formulating it into a hydrogel.…”

Section: Introductionsupporting

confidence: 47%

Hesperidin Hydrogel Formulation Using Pectin-Chitosan Polymer Combination

Fahrurroji

Thendriani

Riza

2017

Int J Pharm Pharm Sci

View full text Add to dashboard Cite

Objective: Hesperidin, a flavonoid glycosides that have been proven to have therapeutic activity to some disease, one of them is colon disease; in addition of its efficacy,low solubility(<100 mg/l) makes hesperidin slightly absorbed,hence it needs a delivery system which could deliver hesperidin to its therapeutic target. This research aims to obtain an optimum formula for pectin polymer combination which can regulate in vitro hesperidin release. Methods:Determination of optimum hydrogel formula uses Design Expert 7.0.0 with factorial method design, resulting in pectin-chitosan concentration formula plan-comparison, which are (P3%: C1%), (P3%: C2%), (P5%: C1%), (P5%: C2%) respectively. Hydrogel was obtained from a variety of formulas,then evaluation of the entrapment efficiency test, swelling index, in vitro drug release test, mucoadhesive strength were conducted.Results: Optimum formula with: pectin: chitosan concentration comparison (5: %: 1%) have an entrapment efficiency of 96.658%; k (/hour) swelling index at pH 5.0, 6.8, and 7.4, was 34.917, 15.766, and 8.146 respectively; drug release at pH 5.0, 6.8, and a medium contained 2% rat caecum was 0.461, 20.116, and the mucoadhesive strength was 0.184 N/cm 2 Conclusion: Combination of pectin-chitosan polymer in hydrogel mucoadhesive regulates hesperidin shesperidin in vitro release, with highest drug release in medium containing 2% rats caecum which releases 56% of active substance. Hesperidin hydrogel release mechanism follows Higuchi kinetics. The optimum hesperidin hydrogel formula is the formula with 5% of pectin and 1% of chitosan. Based on experimental data value which uses simplex lattice design, optimum hesperidin hydrogel formula has insignificant difference between observed and predicted value (p value>0.05).. Based on the test result using independent t-test sample, actual and prediction value from every test parameter produced by the optimum formula was not significantly different with p-value>0,05.

show abstract

Direct evidence of oxidative damage in acute and chronic phases of experimental colitis in rats

Cited by 77 publications

References 26 publications

Effect of different doses of Manuka honey in experimentally induced inflammatory bowel disease in rats

Effect of different doses of Manuka honey in experimentally induced inflammatory bowel disease in rats

Effects of Morin on an Experimental Model of Acute Colitis in Rats

Hesperidin Hydrogel Formulation Using Pectin-Chitosan Polymer Combination

Contact Info

Product

Resources

About