Effect of different doses of Manuka honey in experimentally induced inflammatory bowel disease in rats

Abstract

To evaluate the effect of different doses of Manuka honey in experimentally induced inflammatory bowel disease in rats. Adult Wistar rats of either sex were used (n = 30). Colitis was induced by a single intracolonic administration of TNBS dissolved in 35% ethanol. The rats (n = 30) were divided into five groups (n = 6) and were treated with vehicle (ethanol), TNBS, Manuka honey (5 g/kg, p.o.), Manuka honey (10 g/kg, p.o.) or sulfasalazine (360 mg/kg, p.o.) body weight for 14 days. After completion of treatment, the animals were killed and the following parameters were assessed: morphological score, histological score and different antioxidant parameters.Manuka honey at different doses provided protection against TNBS-induced colonic damage. There was significant protection with Manuka honey 5 g/kg as well as with 10 g/kg body weight compared with the control (p < 0.001). All the treated groups showed reduced colonic inflammation and all the biochemical parameters were significantly reduced compared with the control in the Manuka honey treated groups (p < 0.001). Manuka honey at different doses restored lipid peroxidation as well as improved antioxidant parameters. Morphological and histological scores were significantly reduced in the low dose Manuka honey treated group (p < 0.001). In the inflammatory model of colitis, oral administration of Manuka honey 5 g/kg and Manuka honey 10 g/kg body weight significantly reduced the colonic inflammation. The present study indicates that Manuka honey is efficacious in the TNBS-induced rat colitis model, but these results require further confirmation in human studies.

Protective Effects of Honey and Spirulina Platensis on Acetic Acid-Induced Ulcerative Colitis in Rats

“…Interactions between environmental factors and genetic and immune responses may account for the overproduction of free radicals, reactive oxygen species (ROS), and proinflammatory cytokines, which start inflammatory responses to involve in the pathogenesis of IBD. An imbalance between oxidant/antioxidant systems results in oxidative stress and inflammatory cascade (4)(5)(6).…”

“…In addition, natural honey, as a good source of phenolic and flavonoid compounds, is a powerful antioxidant, which may be effective in preventing chronic oxidative stress, as well as subsequent inflammation (5).…”

Honey potentially mitigates morphine analgesic tolerance and physical dependence in rats

“…Remarkably, honey is a natural medication for the dealing of gastrointestinal problems. Honey has inhibitory results on colonic motility through direct motion on lean muscle tissues and has the potential to reduce fluid secretion 29,30,31,32 . Therefore, honey seems to be positive all through the withdrawal duration for addicted patients with gastrointestinal hyperactivity and diarrhea.…”

Comparison of Manuka, Kanuka, and Black Locust Honey on the Production of Chemical Mediators by Peripheral Blood Mononuclear Cells

“…PBMCs were isolated by density gradient centrifugation on Histopaque (Sigma, St. Louis, MO, USA). Cells from the interphase were harvested, washed and resuspended at 2×10 6 Interleukin-5 (IL-5), IL-10, interferon-γ (INF-γ), and tumor necrosis factor-α (TNF-α) levels in supernatant were measured to determine the anti-inflammatory effect of honey in duplicate using commercially available enzyme-linked immunosorbent assay kit (Endogen, Wobrun, MA, USA) according to the manufacturer's instruction. The sensitivities of this kit were 2 pg/mL for these cytokines.…”

“…5) Oral administration of honey has been shown to decrease inflammatory bowel disease, indomethacin-induced gastritis, and radiation induced oral mucositis. [6][7][8] Black locust honey is one of the most popular unifloral types of honey and commonly produced during spring season in Korea. Korean black locust honey suppressed bac-terial growth and their antibacterial activity was associated with the content of hydrogen peroxide.…”