Direct Evidence of Interaction of a Green Tea Polyphenol, Epigallocatechin Gallate, with Lipid Bilayers by Solid-state Nuclear Magnetic Resonance

Effect of Dietary Green Tea Catechin Preparation on Oxidative Stress Parameters in Large Intestinal Mucosa of Rats

“…We have suggested that (-)-EC and (-)-EGC are localized near the surface of phospholipid membranes for scavenging aqueous oxygen radicals and thereby preventing the consumption of lipophilic vitamin E in a liposomal model membrane system. 13) Hence interaction of GTCs with the membranes, as indicated by Kumazawa et al, 14) facilitate protection of vitamin E from ROS attack on the luminal side. Table 3 shows the result of TBARS assay for the large intestinal mucosa homogenate of each group.…”

Quercetin and epigallocatechin gallate effects on the cell membranes biophysical properties correlate with their antioxidant potential

“…Several studies investigated the interaction between the two polyphenols and the cell membrane using both artificial membranes (Kajiya et al 2002;Kumazawa et al 2004;Tamba et al 2007;Kamihara et al 2008;Iftime et al 2010) and cell lines (Kanupriya et al 2006;Verstraeten et al 2008) and the results showed that flavonoids induced changes at the cell membrane level that lead to an increased protection against oxidative stress.…”

Solid‐state NMR analysis of the orientation and dynamics of epigallocatechin gallate, a green tea polyphenol, incorporated into lipid bilayers

“…Our previous work indicated that the epigallocatechin gallate (EGCg) molecule is incorporated into lipid bilayers by adopting an unique orientation, perturbing head-group motion and the conformation of the phospholipids. [10] However, the orientation and motion of EGCg in lipid bilayers have not been well-characterized. In this study, we investigated the orientation and dynamics of EGCg incorporated into multi-lamellar vesicles (MLVs) and bicelles using solid-state 31 P and 2 H NMR spectroscopy.…”