Direct, Electronic MicroRNA Detection for the Rapid Determination of Differential Expression Profiles

An electrochemical ELISA-like immunosensor for miRNAs detection based on screen-printed gold electrodes modified with reduced graphene oxide and carbon nanotubes

“…Current methods for miRNA detection, as quantitative reverse transcription polymerase chain reaction (qRT-PCR), Northern blotting and DNA array technology, only partially meet the requirements needed for routine detection. That is why developing miRNA detection and quantification tools is challenging (Dong et al, 2012a;Yang et al, 2009;Yin et al, 2012;Zhang et al, 2009;Tran et al, 2013b;2014;Fang et al, 2006). To develop highly sensitive miRNA biosensors, e.g.…”

“…To develop highly sensitive miRNA biosensors, e.g. bioanalytical tools able to detect miRNA below the nanomolar level, many strategies based on nanomaterials have been applied, using graphene (Dong et al, 2012a), silicon nanowires (Yang et al, 2009;Zhang et al, 2009), gold nanoparticles (Roy et al, 2011;Yin et al, 2012), silver nanoclusters (Dong et al, 2012b) or conducting polymer/carbon nanotube hybrids (Tran et al, 2013b). For example, Zhang et al (2009) have developed a label-free electrochemical biosensor based on silicon nanowires.…”

“…8 fM. Yang et al (2009) andFang et al (2009) have developed microelectrodes based on Pt nanostructures for immobilization of DNA-SH probes to detect miRNAs by SWV in phosphate buffer containing redox indicators [Ru (NH 3 ) 6 ] 3 þ or [Fe(CN) 6 ] 3-. These systems, which imply a reagent added in solution, can detect miRNAs at the aM level.…”

Protein Electrocatalysis for Direct Sensing of Circulating MicroRNAs

“…37 Moreover, the Fe-Ru redox pair was used as an electrocatalytic reporter to detect miRNAs with high sensitivity. 38 Furthermore, a series of electrochemical sensors for miRNA analysis have been developed by labeling the target miRNA with OsO 2 , 39 RuO 2 , 40 Os(dmpy) 2 (IN)Cl + nanoparticles. 35 However, these methods require pre-labeling of the target miRNA prior to detection which might complicate the assay, particularly in clinical analysis.…”

Photoelectrochemical biosensing platform for microRNA detection based on in situ producing electron donor from apoferritin-encapsulated ascorbic acid

“…To confirm it, the detection of expression level change of microRNA in poultry infecting with ALVs is necessary. So far, many techniques have been developed for microRNA detection, such as fluorescence (Yang et al, 2012), isothermal amplification , RT-PCR (Huang et al, 2009), microarrays (Yan et al, 2008), bioluminescence assay (Cissell et al, 2008), and electrochemistry (Pöhlmann and Sprinzl, 2010;Wu et al, 2013;Yang et al, 2009), etc. Though these methods have their own merits, the development of new detection technology for microRNA with high sensitivity and selectivity is still a challenge.…”