Photoelectrochemical biosensing platform for microRNA detection based on in situ producing electron donor from apoferritin-encapsulated ascorbic acid

Yin, Huanshun; Wang, Mo; Zhou, Yunlei; Zhang, Xiaoyan; Sun, Bing; Wang, Guihua; Ai, Shiyun

doi:10.1016/j.bios.2013.09.053

Cited by 45 publications

(23 citation statements)

References 46 publications

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“…As reported in our previous works [19][20][21][22], the photocurrent will decrease when the AuNPs were immobilized on Bi 2 S 3 / ITO electrode (named as AuNPs/Bi 2 S 3 /ITO), which could decrease the detection sensitivity. In order to overcome this deficiency, we used Bi 2 S 3 -AuNPs-modified ITO electrode (Bi 2 S 3 -AuNPs/ITO) as substrate electrode instead of AuNPs/Bi 2 S 3 / ITO.…”

Section: Characterization Of Aunps-bi 2 Smentioning

confidence: 90%

“…AuNPs were synthesized according to a previous report [28]. Bi 2 S 3 nanorods were prepared according to a previous report with some modifications as described in our previous work [20]. Indium tin oxide (ITO) was offered by Zhuhai Kaivo Electronic Components Co., Ltd. (Zhuhai, China, ITO coating 180±25 nm, sheet resistance <15 Ω/cm 2 ).…”

Section: Methodsmentioning

confidence: 99%

“…AuNPs-Bi 2 S 3 preparation Bi 2 S 3 nanorods were synthesized according to a previous report [20]. For the synthesis of AuNPs-Bi 2 S 3 composite material, 660 mg of Bi 2 S 3 nanorods were first dispersed into 99.78 mL double-distilled deionized water with the aid of ultrasonication for 1 h. Then, 0.22 mL 440 mM HAuCl 4 was added into the above dispersion solution and further ultrasonicated for 30 min.…”

Section: Methodsmentioning

confidence: 99%

“…Encouraged by the application in DNA detection, we think that the PEC technique for microRNA detection is feasible. Unfortunately, there are few reports on the application of PEC assay for microRNA detection except some work that has been done by our group [19][20][21][22].…”

Section: Introductionmentioning

confidence: 99%

“…Ren et al developed a sensitive and selective label-free microRNA biosensor on the basis of hybridized target microRNA strandinitiated cleavage of hybridized DNA capture probes by DSN, then microRNA was detected according to the change of electrochemical impedance of the biosensor [27]. Inspired by these detection strategies, we developed a novel "signaloff" PEC biosensor for microRNA detection based on DSN signal amplification and in situ enzymatic generation of electron donor using Bi 2 S 3 -gold composite nanomaterial (AuNPsBi 2 S 3 ) as photoactive material, where the photoactivity of Bi 2 S 3 has been demonstrated in our previous reports [19,20,22], and AuNPs on the surface of Bi 2 S 3 nanorods were used as photoelectronic transfer promoter and DNA probe immobilization carrier.…”

Section: Introductionmentioning

confidence: 99%

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Photoelectrochemical biosensor for highly sensitive detection of microRNA based on duplex-specific nuclease-triggered signal amplification

Wang

et al. 2015

J Solid State Electrochem

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A selective and sensitive photoelectrochemical assay was proposed for microRNA detection based on the isothermal cycle hybridization amplification of microRNA-21 that originated from the specific cleavage activity of duplexspecific nuclease (DSN) toward a DNA probe in DNA-RNA double helix and in situ enzymatic production of electron donor of ascorbic acid. After the biotin-functionalized DNA probe hybridized with complementary target microRNA-21, the hybridized DNA probe could be cleaved by DSN and microRNA-21 was released back to the incubation solution. The released microRNA could be further hybridized with the remaining single-strand DNA probe; thus, the isothermal cycle hybridization amplification would be formed. As a result, little avidin-alkaline phosphatase conjugate could be captured due to the release of biotin originating from the DSN cleavage. The distinct photocurrent change between a control biosensor and the DSN cleavage biosensor achieved label-free microRNA-21 detection with the linear range from 1 to 500 fM. The fabricated biosensor showed high detection selectivity even for one-base mismatched sequence. The attempt was carried out to directly assay the expression level change of microRNA-21 in total RNA extracted from chicken fibroblast cells infected with subgroup J avian leukosis virus.

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Section: Characterization Of Aunps-bi 2 Smentioning

confidence: 90%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Photoelectrochemical biosensor for highly sensitive detection of microRNA based on duplex-specific nuclease-triggered signal amplification

Wang

et al. 2015

J Solid State Electrochem

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Synthetic biology principles for the design of protein with novel structures and functions

2020

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Nature provides a large number of functional proteins that evolved during billions of years of evolution. The diversity of natural proteins encompasses versatile functions and more than a thousand different folds, which, however, represents only a tiny fraction of all possible folds and polypeptide sequences. Recent advances in the rational design of proteins demonstrate that it is possible to design de novo protein folds unseen in nature. Novel protein topologies have been designed based on similar principles as natural proteins using advanced computational modelling or modular construction principles, such as oligomerization domains. Designed proteins exhibit several interesting features such as extreme stability, designability of 3D topologies and folding pathways. Moreover, designed protein assemblies can implement symmetry similar to the viral capsids, while, on the other hand, single-chain pseudosymmetric designs can address each position independently. Recently, the design is expanding towards the introduction of new functions into designed proteins, and we may soon be able to design molecular machines.

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Nanotechnology‐Based Strategies for the Detection and Quantification of MicroRNA

Degliangeli

Pompa

Fiammengo

2014

Chemistry A European J

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MicroRNAs (miRNAs) are important regulators of gene expression, and many pathological conditions, including cancer, are characterized by altered miRNA expression levels. Therefore, accurate and sensitive quantification of miRNAs may result in correct disease diagnosis establishing these small noncoding RNA transcripts as valuable biomarkers. Aiming at overcoming some limitations of conventional quantification strategies, nanotechnology is currently providing numerous significant alternatives to miRNA sensing. In this review an up-to-date account of nanotechnology-based strategies for miRNA detection and quantification is given. The topics covered are: nanoparticle-based approaches in solution, sensing based on nanostructured surfaces, combined nanoparticle/surface sensing approaches, and single-molecule approaches.

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Photoelectrochemical biosensing platform for microRNA detection based on in situ producing electron donor from apoferritin-encapsulated ascorbic acid

Cited by 45 publications

References 46 publications

Photoelectrochemical biosensor for highly sensitive detection of microRNA based on duplex-specific nuclease-triggered signal amplification

Photoelectrochemical biosensor for highly sensitive detection of microRNA based on duplex-specific nuclease-triggered signal amplification

Synthetic biology principles for the design of protein with novel structures and functions

Nanotechnology‐Based Strategies for the Detection and Quantification of MicroRNA

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