Direct electron transfer of Horseradish peroxidase on porous structure of screen-printed electrode

Teng, Yuanjie; Zuo, Shaohua; Lan, Minbo

doi:10.1016/j.bios.2008.07.062

Cited by 70 publications

(18 citation statements)

References 34 publications

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“…The K M value of the H 2 O 2 sensor was found to be 79 μmol L −1 , which was lower and near than the corresponding values observed for HRP immobilized on indium tin oxide electrode [29], gold electrode [37], porous SPCE [31] and glass carbon electrode [22]. The low K M value indicated that the present HRP/BSA/SPCNTE exhibited high biologically affinity to H 2 O 2 .…”

Section: Amperometric Response Of the Disposable Hydrogen Peroxide Bicontrasting

confidence: 54%

A third-generation biosensor for hydrogen peroxide based on the immobilization of horseradish peroxidase on a disposable carbon nanotubes modified screen–printed electrode

Qin

Zhang

et al. 2015

Microchim Acta

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A screen-printed carbon nanotube (CNTs) based disposable third generation biosensor for hydrogen peroxide (H 2 O 2 ) was constructed by mixing CNTs with a cellulose acetate binder on an epoxy substrate. The surface was covered with a layer consisting of horseradish peroxidase (HRP) crosslinked to bovine serum albumin with glutaraldehyde. The CNTs acted as both the electrode material and charge promoter to facilitate the direct electron transfer between immobilized HRP and the modified electrode. At a working potential of −300 mV (vs. Ag/AgCl), the biosensor displays an excellent electrocatalytic response to the reduction of H 2 O 2 without the aid of a mediator. It has a fast (3 s) amperometric response and a linear calibration in the concentration range 0.005-0.1 mmol L −1 , with a 0.85 μM detection limit (at an S/ N of 3). The biosensor can be easily produced, is stable and reproducible.

show abstract

Section: Amperometric Response Of the Disposable Hydrogen Peroxide Bicontrasting

confidence: 54%

A third-generation biosensor for hydrogen peroxide based on the immobilization of horseradish peroxidase on a disposable carbon nanotubes modified screen–printed electrode

Qin

Zhang

et al. 2015

Microchim Acta

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show abstract

“…The manufacture of SPCE had been described in detail in our previous work [28,29], with a designed working area of 0.071 cm 2 . During the fabrication of modified electrodes, the pre-treated CNTs, N-CNTs, GrNRs and N-GrNRs were well dispersed in water via ultrasonication with a concentration of 1 mg mL −1 , respectively.…”

Section: Fabrication Of Modified Electrodesmentioning

confidence: 99%

Electrocatalytic sensing of hydrogen peroxide using a screen printed carbon electrode modified with nitrogen-doped graphene nanoribbons

et al. 2015

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We have synthesized nitrogen-doped graphene nanoribbons (N-GrNRs) by unzipping multi-walled carbon nanotubes (CNTs) under strongly oxidizing conditions and subsequent doping with nitrogen by a low-temperature hydrothermal method. The N-GNRs were characterized by transmission electron microscopy, X-ray diffraction, X-ray photoelectron spectroscopy and Raman spectroscopy, and assembled on a disposable screen-printed carbon electrode to give a sensor for H 2 O 2 that was characterized by cyclic voltammetry, electrochemical impedance spectroscopy, chronocoulometry and chronoamperometry. The nano-modified electrode displays enhanced electron transfer ability, and has a large active surface and a large number of catalytically active sites that originate from the presence of nitrogen atoms. This results in a catalytic activity towards H 2 O 2 reduction at near-neutral pH values that is distinctly improved compared to electrodes modified with CNTs or unzipped (nondoped) CNTs only. At a working potential of −0.4 V (vs. Ag/AgCl), the amperometric responses to H 2 O 2 cover the 5 to2785 μM concentration range, with a limit of detection as low as 1.72 μM. This enzyme-free electrochemical sensor exhibits outstanding selectivity and long-term stability for H 2 O 2 detection.

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“…MnO 2 is a promising material due to its superior electrochemical performance, environmentally friendly nature and low cost [25]. In this paper, Nafion is used as a kind of immobilization material to form the Nafion film which has high chemical stability, good biocompatibility and the ability to resist interferences from anions and biological macromolecules [26][27][28]. The performance of the prepared MnO 2 /Nafion/GCE and application of this modified electrode in the determination of EP have been investigated in detail.…”

Section: Introductionmentioning

confidence: 99%

Highly sensitive determination of epinephrine by a MnO2/Nafion modified glassy carbon electrode

Liu¹,

Ye²,

Luo³

et al. 2012

Journal of Electroanalytical Chemistry

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Direct electron transfer of Horseradish peroxidase on porous structure of screen-printed electrode

Cited by 70 publications

References 34 publications

A third-generation biosensor for hydrogen peroxide based on the immobilization of horseradish peroxidase on a disposable carbon nanotubes modified screen–printed electrode

A third-generation biosensor for hydrogen peroxide based on the immobilization of horseradish peroxidase on a disposable carbon nanotubes modified screen–printed electrode

Electrocatalytic sensing of hydrogen peroxide using a screen printed carbon electrode modified with nitrogen-doped graphene nanoribbons

Highly sensitive determination of epinephrine by a MnO2/Nafion modified glassy carbon electrode

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