Direct Electrochemistry of Hemoglobin and Its Electrocatalysis Based on Hyaluronic Acid and Room Temperature Ionic Liquid

Gao, Ruifang; Shangguan, Xiaodong; Qiao, Guang-Jun; Zheng, Jianbin

doi:10.1002/elan.200804353

Cited by 36 publications

(21 citation statements)

References 42 publications

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“…Although a larger signal was obtained at pH 8.5, PBS at pH 7.3 was selected as the supporting electrolyte in subsequent experiments, given that Hb sensors would be normally used under physiological conditions. A similar overall shift in bioelectrocatalytic wave for oxygen reduction with pH was also observed by Gao et al [19] who showed that the peak reduction potential (vs SCE) shifted from -500mV to300mV (ie -455mV to -255mV vs Ag/AgCl) when changing the pH from 9 to 5 respectively. In our study of a 4 unit reduction in pH, we observed a comparable shift from -480mV to -350mV (vs Ag/AgCl).…”

Section: Ph Dependency Of Haemoglobin Electrochemical Signalsupporting

confidence: 82%

Electrochemical probing of selective haemoglobin binding in hydrogel-based molecularly imprinted polymers

Reddy

Sette

Phan

2011

Electrochimica Acta

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An electrochemical method has been developed for the probing of hydrogel-based molecularly imprinted polymers (HydroMIPs) on the surface of a glassy carbon electrode. HydroMIPs designed for bovine haemoglobin selectivity were electrochemically characterized and their rebinding properties were monitored using cyclic voltammetry. The electrochemical reduction of bovine oxyhaemoglobin (BHb) in solution was observed to occur at -0.460 V vs (Ag/AgCl) in 150mM phosphate buffer solution (PBS). When the protein was selectively bound to the MIP, the electrochemical reduction of oxyhameoglobin could be observed at a similar peak potential of -0.480 V vs (Ag/AgCl). When analysing the non-imprinted control polymer (NIP) interfaced at the electrode, which contained no protein, the peak reduction potential corresponded to that observed for dissolved oxygen in solution (-0.65 V vs (Ag/AgCl)). MIP and NIP (in the absence of protein) were interfaced at the electrode and protein allowed to diffuse through the polymers from the bulk solution end to the electrode. It was observed that whereas NIP exhibited a protein response within 10 minutes of protein exposure, up to 45 min of exposure time was required in the case of the MIP before a protein response could be obtained. Our results suggest that due to the selective nature of the MIP, BHb arrival at the electrode via diffusion is delayed by the MIP due to attractive selective interactions with exposed cavities, but not the NIP which is devoid of selective cavities.

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Section: Ph Dependency Of Haemoglobin Electrochemical Signalsupporting

confidence: 82%

Electrochemical probing of selective haemoglobin binding in hydrogel-based molecularly imprinted polymers

Reddy

Sette

Phan

2011

Electrochimica Acta

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“…28 Since HA is a naturally occurring biopolymer, it is biocompatible and biodegradable, 29 and it has been utilized as an immobilization matrix for biosensors and biocatalysis. [30][31][32][33] The present paper focused on (1) 6 and β-D(＋)-glucose were of analytical grades and used without further purification. 0.1 mol/L phosphate buffer solution (PBS) was used as the supporting electrolyte.…”

Section: Introductionmentioning

confidence: 99%

Direct Electrochemistry and Electrocatalysis Behaviors of Glucose Oxidase Based on Hyaluronic Acid‐Carbon Nanotubes‐Ionic Liquid Composite Film

et al. 2010

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Multi-walled carbon nanotubes (MWNTs) were dispersed in the ionic liquid [BMIM] [BF 4 ]-MWNTs could obviously improve the diffusion of ferricyanide toward the electrode surface. The experimental results of CV showed that a pair of well-defined and quasi-reversible peaks of GOx at the modified electrode was exhibited, and the redox reaction of GOx at the modified electrode was surface-confined and quasi-reversible electrochemical process. The average surface coverage of GOx and the apparent Michaelis-Menten constant were 8.5×10-9 mol/cm 2 and 9.8 mmol/L, respectively. The cathodic peak current of GOx and the glucose concentration showed linear relationship in the range from 0.1 to 2.0 mmol/L with a detection limit of 0.03 mmol/L (S/N＝3). As a result, the method presented here could be easily extended to immobilize and obtain the direct electrochemistry of other redox enzymes or proteins.

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“…In recent years, more and more attention has been paid to the electrochemical behaviors of redox proteins entrapped in room temperature ionic liquids (RTILs) based matrixes [42][43][44][45] due to the excellent physiochemical properties of RTILs, such as high ionic conductivity and wide potential windows. 46,47 The combination of RTIL with conventional matrices can create unique materials, so they have been extensively used to prepare modified electrodes and biosensors in electroanalysis.…”

Section: Introductionmentioning

confidence: 99%

Direct Electrochemistry of Hemoglobin Immobilized on Hydrophilic Ionic Liquid-chitosan-ZrO2 Nanoparticles Composite Film with Carbon Ionic Liquid Electrode as the Platform

2010

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Direct Electrochemistry of Hemoglobin and Its Electrocatalysis Based on Hyaluronic Acid and Room Temperature Ionic Liquid

Cited by 36 publications

References 42 publications

Electrochemical probing of selective haemoglobin binding in hydrogel-based molecularly imprinted polymers

Electrochemical probing of selective haemoglobin binding in hydrogel-based molecularly imprinted polymers

Direct Electrochemistry and Electrocatalysis Behaviors of Glucose Oxidase Based on Hyaluronic Acid‐Carbon Nanotubes‐Ionic Liquid Composite Film

Direct Electrochemistry of Hemoglobin Immobilized on Hydrophilic Ionic Liquid-chitosan-ZrO2 Nanoparticles Composite Film with Carbon Ionic Liquid Electrode as the Platform

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