Direct electrochemical detection of kanamycin based on peroxidase-like activity of gold nanoparticles

Wang, Chunshuai; Liu, Chang; Luo, Jibao; Tian, Yaping; Zhou, Nandi

doi:10.1016/j.aca.2016.07.013

Cited by 99 publications

(50 citation statements)

References 43 publications

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“…The result was consistent with other reported detection methods of kanamycin based on the specific DNA aptamer of kanamycin. 14,16,17,32 The above results showed that our proposed label-free and turn-on fluorescence strategy had a satisfactory selectivity of kanamycin over that of other antibiotic drugs.…”

Section: Introductionmentioning

confidence: 61%

A Label-free and Turn-on Fluorescence Strategy for Kanamycin Detection Based on the NMM/G-quadruplex Structure

Yang

et al. 2017

ANAL. SCI.

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In the present work, a simple design of a "label-free" fluorescence method for kanamycin was developed and explored based on a specific DNA aptamer as a recognizer and a NMM/G-quadruplex DNA system as a reporter. The titration experiment showed a linear detection range of 0.5 to 100 nM with a low detection limit of 0.5 nM. It also showed excellent selectivity in a selectivity experiment. It was then successfully employed to detect kanamycin in milk with a excellent reliability.

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Section: Introductionmentioning

confidence: 61%

A Label-free and Turn-on Fluorescence Strategy for Kanamycin Detection Based on the NMM/G-quadruplex Structure

Yang

et al. 2017

ANAL. SCI.

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“…Such a detection approach leading to a much improved signal‐amplified response . Herein, AuNPs are not only used as carriers of thionine mediator, but also catalyze the electrochemical reaction between H 2 O 2 and TH red to produce TH ox . Thus, modification of the transducer surface with AuNPs provides a biocompatible interface with a higher conductivity and catalytic activity, that further amplifies the electrochemical signal.…”

Section: Resultsmentioning

confidence: 99%

A Prostate Specific Antigen Immunosensor Based on Biotinylated‐Antibody/Cyclodextrin Inclusion Complex: Fabrication and Electrochemical Studies

et al. 2017

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Immobilization of biomolecules with a proper orientation is considered as a basis for diverse biotechnological applications. Herein, we report a host‐guest inclusion complexation between β‐cyclodextrin (β‐CD) and biotin as a versatile approach for the immobilization of biomolecules. As a practical application, a sandwich‐type electrochemical immunosensor was designed for the determination of prostate specific antigen (PSA). The immunosensor was fabricated by in situ electropolymerization of poly(N‐acetylaniline) onto a rGO‐modified Pt electrode. Then, β‐CD was covalently grafted onto the over‐oxidized polymer backbone. For improving the efficiency of the assay, AuNPs were casted on the polymeric film, on the surface of which thionine (TH) as an electron mediator was covalently immobilized. Using a host‐guest inclusion complexation between β‐CD and biotin, a β‐CD/biotin‐Ab1/PSA/Ab2‐horseradish peroxidase (HRP) sandwich was formed on the electrode surface. The analytical signal was produced via electrochemical reduction of THox, generated by biocatalytic oxidation of the THred in the presence of HRP/H2O2. Under optimal conditions, the proposed sensor responded linearly to PSA in the range from 10.0 pg mL−1 to 25.0 ng mL−1, with a low detection limit of 6.7 pg mL−1 (S/N=3). Kinetic parameters of the interaction of β‐CD with Ab1 were also investigated. Finally, the applicability of the immunosensor was successfully investigated for the detection of PSA in human serum samples.

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“…Over the past decade, nanozymes, i.e., artificial nanomaterials (metal oxide, metal, and carbon-based materials or their nanohybrids) with inherent activity similar to enzymes are gaining attraction as catalytic nanocarriers/labels [ 19 , 32 , 33 ] or electrode surface modifiers [ 34 , 35 , 36 ] in electrochemical affinity biosensing. Particularly, nanozymes with peroxidase-like activity are highly stable and low-cost alternatives to enzymes [ 9 , 37 ].…”

Section: Selected Nanostructures In Electrochemical Affinity Biosementioning

confidence: 99%

“…Particular attention is given to the type of nanostructures and their role, target analyte, electrochemical technique and reported application. Although immunosensors and DNA sensors have been mostly prepared, aptasensors and others have been developed for the determination of: food allergens [ 18 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 ]; gluten [ 69 , 70 ]; GMOs [ 10 , 20 , 21 , 23 ]; toxins [ 32 , 71 , 72 ]; antibiotics [ 34 , 59 , 73 , 74 ]; pesticide residues [ 57 ]; bacteria [ 19 , 26 , 36 ]; fungus [ 22 , 75 ]; and yeast [ 76 ]. The biosensors were developed either in integrated formats or by coupling MNPs to conventional or screen-printed electrode substrates.…”

Section: Selected Nanostructures In Electrochemical Biosensing Formentioning

confidence: 99%

Electrochemical Affinity Biosensors Based on Selected Nanostructures for Food and Environmental Monitoring

Campuzano

Yáñez‐Sedeño

Pingarrón

2020

Sensors

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The excellent capabilities demonstrated over the last few years by electrochemical affinity biosensors should be largely attributed to their coupling with particular nanostructures including dendrimers, DNA-based nanoskeletons, molecular imprinted polymers, metal-organic frameworks, nanozymes and magnetic and mesoporous silica nanoparticles. This review article aims to give, by highlighting representative methods reported in the last 5 years, an updated and general overview of the main improvements that the use of such well-ordered nanomaterials as electrode modifiers or advanced labels confer to electrochemical affinity biosensors in terms of sensitivity, selectivity, stability, conductivity and biocompatibility focused on food and environmental applications, less covered in the literature than clinics. A wide variety of bioreceptors (antibodies, DNAs, aptamers, lectins, mast cells, DNAzymes), affinity reactions (single, sandwich, competitive and displacement) and detection strategies (label-free or label-based using mainly natural but also artificial enzymes), whose performance is substantially improved when used in conjunction with nanostructured systems, are critically discussed together with the great diversity of molecular targets that nanostructured affinity biosensors are able to quantify using quite simple protocols in a wide variety of matrices and with the sensitivity required by legislation. The large number of possibilities and the versatility of these approaches, the main challenges to face in order to achieve other pursued capabilities (development of antifouling, continuous operation, wash-, calibration- and reagents-free devices, regulatory or Association of Official Analytical Chemists, AOAC, approval) and decisive future actions to achieve the commercialization and acceptance of these devices in our daily routine are also noted at the end.

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Direct electrochemical detection of kanamycin based on peroxidase-like activity of gold nanoparticles

Cited by 99 publications

References 43 publications

A Label-free and Turn-on Fluorescence Strategy for Kanamycin Detection Based on the NMM/G-quadruplex Structure

A Label-free and Turn-on Fluorescence Strategy for Kanamycin Detection Based on the NMM/G-quadruplex Structure

A Prostate Specific Antigen Immunosensor Based on Biotinylated‐Antibody/Cyclodextrin Inclusion Complex: Fabrication and Electrochemical Studies

Electrochemical Affinity Biosensors Based on Selected Nanostructures for Food and Environmental Monitoring

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