A Label-free and Turn-on Fluorescence Strategy for Kanamycin Detection Based on the NMM/G-quadruplex Structure

Yang, Hualin; Wu, Qinghua; Su, Dongxiao; Wang, Yun; Li, Li; Zhang, Xingping

doi:10.2116/analsci.33.133

Cited by 13 publications

(5 citation statements)

References 32 publications

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“…Organic fluorescent groups including crystal violet (CV) ( Kong et al, 2009 ), thiazole orange (TO) ( Lubitz et al, 2010 ), protoporphyrin IX (PPPIX) ( Zhang et al, 2012 ), thioflavin T (ThT) ( Mohanty et al, 2013 ) and their analogues ( Kataoka et al, 2014 ), metal complexes such as Pt (II), IR (III) and Ru (II) complexes ( Wang et al, 2015b ), aggregation induced emission (AIE) substances ( Li et al, 2018 ), and other groups with fluorescence properties are used as fluorescence signals for G-quadruplex biosensor. For example, the fluorescence signal of NMM itself is very weak, but can produce strong fluorescence after binding to G-quadruplex ( Kreig et al, 2015 ; Yang et al, 2017 ). TO was shown to be a good G-quadruplex selective fluorescent probe for the design of novel nucleic acid biosensor ( Lu et al, 2015 ).…”

Section: G-quadruplex Based Biosensormentioning

confidence: 99%

G-quadruplex based biosensor: A potential tool for SARS-CoV-2 detection

Juhas

Zhang

2020

Biosensors and Bioelectronics

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G-quadruplex is a non-canonical nucleic acid structure formed by the folding of guanine rich DNA or RNA. The conformation and function of G-quadruplex are determined by a number of factors, including the number and polarity of nucleotide strands, the type of cations and the binding targets. Recent studies led to the discovery of additional advantageous attributes of G-quadruplex with the potential to be used in novel biosensors, such as improved ligand binding and unique folding properties. G-quadruplex based biosensor can detect various substances, such as metal ions, organic macromolecules, proteins and nucleic acids with improved affinity and specificity compared to standard biosensors. The recently developed G-quadruplex based biosensors include electrochemical and optical biosensors. A novel G-quadruplex based biosensors also show better performance and broader applications in the detection of a wide spectrum of pathogens, including SARS-CoV-2, the causative agent of COVID-19 disease. This review highlights the latest developments in the field of G-quadruplex based biosensors, with particular focus on the G-quadruplex sequences and recent applications and the potential of G-quadruplex based biosensors in SARS-CoV-2 detection.

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Section: G-quadruplex Based Biosensormentioning

confidence: 99%

G-quadruplex based biosensor: A potential tool for SARS-CoV-2 detection

Juhas

Zhang

2020

Biosensors and Bioelectronics

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“…Compared with ssDNA, dsDNA and triplex, G4 can be highly selective recognized by N-methyl mesoporphyrin IX (NMM), which is a commercially available anionic porphyrin compound with relatively weak fluorescence emission [35]. Interestingly, fluorescence intensity of NMM is enhanced tenfold upon G4 binding [36][37][38][39]. The NMM/G4 DNA system can be utilized as an excellent and label-free signal reporter to develop biosensors [40][41][42].…”

Section: Introductionmentioning

confidence: 99%

A highly sensitive and low-background fluorescence assay for pesticides residues based on hybridization chain reaction amplification assisted by magnetic separation

Yao

Liu

Zhang

et al. 2019

Methods Appl. Fluoresc.

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Due to the concern over food safety, it is important to detect the pesticides residues in agricultural products. Here, a highly sensitive and low background fluorescent strategy for the detection of pesticides residues has been developed. The fluorescence intensity of N-methyl mesoporphyrin IX (NMM) binding G-quadruplex could be turn off because of inhibiting effect of the pesticides on the acetylcholinesterase (AChE) activity. For that, four single-stranded DNAs (named linker, trigger, H1 and H2, respectively) are rational designed and T-Hg-T mismatches duplex DNAs as a recognizer combined with the separation of magnetic beads. The design of hybridization chain reaction (HCR) amplification strategy assisted by magnetic separation has been adopted to improve the detection sensitivity. In the presence of pesticides, the amount of the thiol group generated by hydrolysis reaction of acetylcholine (ACh) is reduced, lead to release of less trigger DNA. Therefor subsequent HCR process is retarded with decreased fluorescence intensity. The reduced fluorescence intensity has a quantitative relationship with the pesticide concentration. The limit of detection of chlorpyrifos was estimated to be 2.0 ng ml−1. It has been applied to detect the pesticides residues in real samples.

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“…Owing to the numerous combinations of the sequence lengths, redox activity microarray technology has been used to screen biosensors [ 14 , 15 ]. Moreover, the DNAzyme activity increases the fluorescence signal intensity of N -methylmesoporphyrin IX (NMM) [ 16 , 17 ]. Raw milk contains several compounds that inhibit redox activity.…”

Section: Introductionmentioning

confidence: 99%

An Aptamer-Based Biosensor for Direct, Label-Free Detection of Melamine in Raw Milk

Kaneko¹,

Horii²,

Akitomi³

et al. 2018

Sensors

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Melamine, a nitrogen-rich compound, has been used as a food and milk additive to falsely increase the protein content. However, melamine is toxic, and high melamine levels in food or in milk can cause kidney and urinary problems, or even death. Hence, the detection of melamine in food and milk is desirable, for which numerous detection methods have been developed. Several methods have successfully detected melamine in raw milk; however, they require a sample preparation before the analyses. This study aimed to develop an aptamer-DNAzyme conjugated biosensor for label-free detection of melamine, in raw milk, without any sample preparation. An aptamer-DNAzyme conjugated biosensor was developed via screening using microarray analysis to identify the candidate aptamers followed by an optimization, to reduce the background noise and improve the aptamer properties, thereby, enhancing the signal-to-noise (S/N) ratio of the screened biosensor. The developed biosensor was evaluated via colorimetric detection and tested with raw milk without any sample preparation, using N-methylmesoporphyrin IX for fluorescence detection. The biosensor displayed significantly higher signal intensity at 2 mM melamine (S/N ratio, 20.2), which was sufficient to detect melamine at high concentrations, in raw milk.

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A Label-free and Turn-on Fluorescence Strategy for Kanamycin Detection Based on the NMM/G-quadruplex Structure

Cited by 13 publications

References 32 publications

G-quadruplex based biosensor: A potential tool for SARS-CoV-2 detection

G-quadruplex based biosensor: A potential tool for SARS-CoV-2 detection

A highly sensitive and low-background fluorescence assay for pesticides residues based on hybridization chain reaction amplification assisted by magnetic separation

An Aptamer-Based Biosensor for Direct, Label-Free Detection of Melamine in Raw Milk

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