Direct Electrical Detection of DNA Hybridization Based on Electrolyte-Gated Graphene Field-Effect Transistor

Ohno, Yasuhide; Okamoto, Shogo; Maehashi, Kenzo; Matsumoto, Kazuhiko

doi:10.7567/jjap.52.110107

Cited by 45 publications

(44 citation statements)

References 31 publications

(42 reference statements)

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“…The change of electric potential of graphene can be explained by the negative electrostatic gating effect. As DNA has a negatively charged triphosphate group, it can modulate the Fermi level of graphene by inducing excess hole carriers and in turn shift cnp to the positive direction [24,25]. Figure 4(a) shows the transfer characteristics of DNA probe-modified G-FETs before and after addition of target RNAs (full-complementary) with different concentration from 0.1 fM to 1 pM.…”

Section: Resultsmentioning

confidence: 99%

RNA Detection Based on Graphene Field-Effect Transistor Biosensor

Tian

Zhang

et al. 2018

Advances in Condensed Matter Physics

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Graphene has attracted much attention in biosensing applications due to its unique properties. In this paper, the monolayer graphene was grown by chemical vapor deposition (CVD) method. Using the graphene as the electric channel, we have fabricated a graphene field-effect transistor (G-FET) biosensor that can be used for label-free detection of RNA. Compared with conventional method, the G-FET RNA biosensor can be run in low cost, be time-saving, and be miniaturized for RNA measurement. The sensors show high performance and achieve the RNA detection sensitivity as low as 0.1 fM, which is two orders of magnitude lower than the previously reports. Moreover, the G-FET biosensor can readily distinguish target RNA from noncomplementary RNA, showing high selectivity for RNA detection. The developed G-FET RNA biosensor with high sensitivity, fast analysis speed, and simple operation may provide a new feasible direction for RNA research and biosensing.

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Section: Resultsmentioning

confidence: 99%

RNA Detection Based on Graphene Field-Effect Transistor Biosensor

Tian

Zhang

et al. 2018

Advances in Condensed Matter Physics

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“…[85] 1-Pyrenebutanoic acid succinimidyl ester (PBASE) was used as a linker to bind the probe DNA to the graphene surface. In experiment process, the researchers determine whether or not the target DNA is complementary to the probe according to the change of I DS .…”

Section: Graphene-fet For Dna Detectionmentioning

confidence: 99%

Label‐Free DNA Sensors Based on Field‐Effect Transistors with Semiconductor of Carbon Materials

et al. 2015

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Over one decade, various DNA sensors of carbon material-based field-effect transistors (FETs) have been intensively developed into an inspiring area of research and technology to substitute for the traditional method, for instance, fluorescence labeling detection. These FET DNA sensors have advantages of: directly read-out electrical signal, no need to label DNA molecule with fluorescer, high sensitivity, facility of miniaturization, simple device preparation process, high signal-to-noise ratio (SNR) and wide detection range. This review gives a comprehensive description on the state-of-the-art carbon-based FET DNA sensors from the aspect of both semiconductor material and structure of device. Several essential points in this research field, including sensitivity, selectivity, stability and challenges are addressed. Optimization of sensing performance and application of these devices are also discussed.

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“…In particular, the graphene field effect transistor [19–21] (GFET) for DNA (deoxyribonucleic acid) sensors has been developed by several research groups. [13,22–25] Recent achievements in the production of monolayer graphene by chemical vapor deposition [1,26–29] (CVD) and mechanical exfoliation [13,18,27] provide a new platform for developing biological sensors. Detection of electrical conduction was similar for all graphene sheets, with the transfer characteristics for graphene oxide and reduced graphene oxide exhibiting a reduced current ratio when compared with that of monolayer graphene prepared by the CVD and exfoliation method.…”

Section: Introductionmentioning

confidence: 99%

Simplified detection of the hybridized DNA using a graphene field effect transistor

Manoharan

Chinnathambi

Jayavel

et al. 2017

Science and Technology of Advanced Materials

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Detection of disease-related gene expression by DNA hybridization is a useful diagnostic method. In this study a monolayer graphene field effect transistor (GFET) was fabricated for the detection of a particular single-stranded DNA (target DNA). The probe DNA, which is a single-stranded DNA with a complementary nucleotide sequence, was directly immobilized onto the graphene surface without any linker. The VDirac was shifted to the negative direction in the probe DNA immobilization. A further shift of VDirac in the negative direction was observed when the target DNA was applied to GFET, but no shift was observed upon the application of non-complementary mismatched DNA. Direct immobilization of double-stranded DNA onto the graphene surface also shifted the VDirac in the negative direction to the same extent as that of the shift induced by the immobilization of probe DNA and following target DNA application. These results suggest that the further shift of VDirac after application of the target DNA to the GFET was caused by the hybridization between the probe DNA and target DNA.

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Direct Electrical Detection of DNA Hybridization Based on Electrolyte-Gated Graphene Field-Effect Transistor

Cited by 45 publications

References 31 publications

RNA Detection Based on Graphene Field-Effect Transistor Biosensor

RNA Detection Based on Graphene Field-Effect Transistor Biosensor

Label‐Free DNA Sensors Based on Field‐Effect Transistors with Semiconductor of Carbon Materials

Simplified detection of the hybridized DNA using a graphene field effect transistor

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