Direct DNA Amplification and Restriction Pattern Analysis of Helicobacter pylori in Patients with Duodenal Ulcer and Their Families

Wang, Jann-Tay; Sheu, Jin‐Chuan; Lin, Jaw‐Town; Wang, Teh‐Hong; Wu, Ming–Shiang

doi:10.1093/infdis/168.6.1544

Cited by 78 publications

(50 citation statements)

References 13 publications

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“…By using DNA typing technique, the same or similar strains of H. pylori have been identified among family members (4,10,16,23). Roma-Giannikou et al (16) supported the intrafamilial transmission by conducting genetic analysis among 32 members of 11 families.…”

Section: Discussionmentioning

confidence: 99%

“…Those previous epidemiological studies, however, were not based on molecular biological analysis of H. pylori strains, but based mainly on serologic tests or the urea breath test; therefore, the evidence for this possible path of infection has been indirect. In only a few studies the same or similar strains of H. pylori have been identified among families by using the DNA typing technique (4,10,16,23).…”

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confidence: 99%

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Five-Year Follow-Up Study of Mother-to-Child Transmission ofHelicobacter pyloriInfection Detected by a Random Amplified Polymorphic DNA Fingerprinting Method

et al. 2005

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Recent studies have speculated on the possible role of the mother in transmitting Helicobacter pylori infection to their children. In an attempt to either prove or disprove this supposition, we investigated the rates of infection of children born to H. pylori-positive mothers from birth to 5 years of age using serology and the stool antigen test. When infection of the children did occur, the strains from the children were compared to those of their mothers using DNA analysis. Sixty-nine of the 350 pregnant mothers (19.7%) had a positive serology for H. pylori. Fifty-one children underwent serological examinations and stool antigen tests at 4 to 6 days after birth, followed by 1, 3, and 6 months. They were continuously given the stool antigen test at 4-to 6-month intervals until the age of 5 years. Gastric juice samples were collected from the infected children and their mothers for culture and DNA analyses using a random amplified polymorphic DNA fingerprinting method. None of the 51 children acquired H. pylori infection during the first year of life. Of the 44 children enrolled in a 5-year follow-up study, five (11%) acquired H. pylori infection. They acquired the infection at the age of 1 year 2 months, 1 year 3 months, 1 year 6 months, 1 year 8 months, and 4 years 4 months. Random amplified polymorphic DNA fingerprinting confirmed that the strains of the five children exhibited DNA fingerprinting patterns identical to those of their mothers. These findings suggest that mother-to-child transmission is the most probable cause of intrafamilial spread of H. pylori.

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confidence: 99%

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Five-Year Follow-Up Study of Mother-to-Child Transmission ofHelicobacter pyloriInfection Detected by a Random Amplified Polymorphic DNA Fingerprinting Method

et al. 2005

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“…Shared strains among individuals indicate person-to-person transmission or acquisition from a common source. Unrelated individuals harbor distinct H. pylori isolates (1), while clonal lineages can be discerned within families (2,7,11,16,20,22,24,29,33,38,39). These observations are in line with familial transmission and an epidemic clonal population structure (37).…”

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confidence: 97%

Concordance ofHelicobacter pyloriStrains within Families

et al. 2003

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Helicobacter pylori infection is typically acquired in early childhood, and a predominantly intrafamilial transmission has been postulated. To what extent family members share the same strains is poorly documented. Our aim was to explore patterns of shared strains within families by using molecular typing. Family members of H. pylori-infected 10-to 12-year-old index children identified in a school survey were invited to undergo gastroscopy. Bacterial isolates were typed with random amplified polymorphic DNA and PCRrestriction fragment length polymorphism of the genes ureA-B, glmM, or flaA. The presence or absence of the cag pathogenicity island, a bacterial virulence factor, was determined by PCR. GelCompar II software, supplemented with visual inspection, was used in the cluster analysis. In 39 families, 104 individuals contributed 208 bacterial isolates from the antrum and corpus. A large proportion, 29 of 36 (81%) of the offspring in a sibship, harbored the same strain as at least one sibling. Mother-offspring strain concordance was detected in 10 of 18 (56%) of the families. Of 17 investigated father-offspring relations in eight families, none were strain concordant. Spouses were infected with the same strains in 5 of 23 (22%) of the couples. Different strains in the antrum and corpus were found in 8 of 104 (8%) of the subjects. Our family-based fingerprinting study demonstrates a high proportion of shared strains among siblings. Transmission between spouses seems to be appreciable. The data support mother-child and sib-sib transmission as the primary transmission pathways of H. pylori.Helicobacter pylori colonizes half of the world's population, and the infection is associated with chronic gastritis, peptic ulcer, gastric cancer, and gastric lymphoma. In-depth knowledge of the transmission patterns may constitute important information for future intervention strategies.In the absence of consistent and verified environmental reservoirs, a predominantly person-to-person transmission has been postulated. H. pylori infection is associated with poor living conditions, and possible transmission routes are fecaloral, oral-oral, or gastro-oral, but firm evidence is lacking (36). The infection clusters in families and is usually acquired in early childhood. A child's risk of being infected is largely determined by the presence or absence of infected family members. Having an infected mother has been suggested to be a more prominent risk factor than having an infected father (30,35). Indications of sib-sib transmission have been reported (14). Transmission between spouses occurs but its significance is unclear (5, 9, 19).Molecular typing of pathogens can corroborate and further characterize the transmission pathways suggested by epidemiologic data based on infection status. Shared strains among individuals indicate person-to-person transmission or acquisition from a common source. Unrelated individuals harbor distinct H. pylori isolates (1), while clonal lineages can be discerned within families (2,7,11,16,20,22,24,2...

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“…The observation of transmissions from a mother to each of her children should be the means by which the route of infection is evaluated such that one can distinguish socially mediated transmission from a strictly vertical pathway. Third, transmission studies have typically used presence/absence measures of H. pylori infection via 13 C urea breath tests (Rothenbacher et al 1999;Tindberg et al 2001), presence/absence of antibodies (Tindberg et al 2001;Samir et al 2004), restriction enzyme digestion of amplified genes (Wang et al 1993), or analyses of peptide sequences (Prewett et al 1992). Although the latter two methods are an advance over 13 C urea breath tests as they allow comparison of H. pylori genotypes in parents and children, these methods do not provide the high resolution of nucleotide-sequencebased studies and are more susceptible to convergent changes.…”

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A Population Genetics Pedigree Perspective on the Transmission of Helicobacter pylori

et al. 2006

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The inference of transmission pathways for medicinally important bacteria is important to our understanding of pathogens. Here we report analyses of transmission in Helicobacter pylori, a major carcinogen. Our study is novel in that the focal community comprises detailed family pedigrees and has a high prevalence of H. pylori. To infer transmission, we performed high-resolution analyses of nucleotide sequences for three genes and accounted for the occurrence of mutation and recombination through the use of simulation modeling. Our results demonstrate that transmission has a strong nonfamilial component potentially the result of a large proportion of infections derived from the community. These results are interesting from both a medical and an evolutionary standpoint. First, efficient control measures and beliefs about the sources of H. pylori infection should be reevaluated. Evolutionarily, our results contradict the hypothesis of strict vertical transmission, presented as an explanation for the strong correlation between human population history and H. pylori diversity. Thus the paradox of persistent phylogenetic structure, despite a permissive mode of transmission and high recombination rates, must be solved elsewhere. Here we consider the potential for recombination events to maintain genetic structure in light of horizontal transmission.

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Direct DNA Amplification and Restriction Pattern Analysis of Helicobacter pylori in Patients with Duodenal Ulcer and Their Families

Cited by 78 publications

References 13 publications

Five-Year Follow-Up Study of Mother-to-Child Transmission ofHelicobacter pyloriInfection Detected by a Random Amplified Polymorphic DNA Fingerprinting Method

Five-Year Follow-Up Study of Mother-to-Child Transmission ofHelicobacter pyloriInfection Detected by a Random Amplified Polymorphic DNA Fingerprinting Method

Concordance ofHelicobacter pyloriStrains within Families

A Population Genetics Pedigree Perspective on the Transmission of Helicobacter pylori

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