Direct determination of serotonin in gut lavage fluid by liquid chromatographic ion trap tandem mass spectrometry

Gregersen, Kine; Frøyland, Livar; Berstad, Arnold; Araujo, Pedro

doi:10.1016/j.talanta.2007.11.043

Cited by 18 publications

(11 citation statements)

References 157 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Liquid chromatography (LC) coupled to electrospray ionization (ESI) and tandem mass spectrometry (MS/MS) is a selective and sensitive tool for the trace analysis of multiple compounds in various matrices. Methods have been developed using LC-MS/MS to quantify melatonin [13], serotonin [13][14][15], HIAA [16,17], IAA [13,[18][19][20], and 3-IS [21]. Amirkhani et al [22] described a method to determine Trp, KYN, and KA in human plasma by capillary LC-MS/MS.…”

Section: Introductionmentioning

confidence: 99%

Quantitative profiling of tryptophan metabolites in serum, urine, and cell culture supernatants by liquid chromatography–tandem mass spectrometry

et al. 2011

View full text Add to dashboard Cite

A sensitive, selective, and comprehensive method for the quantitative determination of tryptophan and 18 of its key metabolites in serum, urine, and cell culture supernatants was developed. The analytes were separated on a C18 silica column by reversed-phase liquid chromatography and detected by electrospray ionization tandem mass spectrometry in positive ion multiple reaction monitoring (MRM) mode, except for indoxyl sulfate which was measured in negative ion MRM mode in a separate run. The limits of detection and lower limits of quantification were in the range of 0.1-50 and 0.5-100 nM, respectively. Fully 13 C isotope-labeled and deuterated internal standards were used to achieve accurate quantification. The applicability of the method to analyze serum, urine, and cell culture supernatants was demonstrated by recovery experiments and the evaluation of matrix effects. Precision for the analysis of serum, urine, and cell culture supernatants ranged between 1.3% and 16.0%, 1.5% and 13.5%, and 1.0% and 17.4%, respectively. The method was applied to analyze changes in tryptophan metabolism in cell culture supernatants from IFN-γ-treated monocytes and immature or mature dendritic cells.

show abstract

Section: Introductionmentioning

confidence: 99%

Quantitative profiling of tryptophan metabolites in serum, urine, and cell culture supernatants by liquid chromatography–tandem mass spectrometry

et al. 2011

View full text Add to dashboard Cite

show abstract

“…Measurement of Ser is important for monitoring neurodegenerative diseases and psychiatric disorders [13][14][15][16][17][18][19][20]. Various methods like HPLC [19,21], reverse-phase ion-pair HPLC [22], capillary electrophoresis [23], fluorescence [24][25][26][27], coulometry [28] and mass spectrometry [29,30] have been used to determine the concentration of Ser. Electrochemical methods include advantages of low cost, high sensitivity and short measurement time.…”

Section: Introductionmentioning

confidence: 99%

A Selective Simultaneous Determination of Levodopa and Serotonin Using a Glassy Carbon Electrode Modified with Multiwalled Carbon Nanotube/Chitosan Composite

Babaei

Babazadeh

2011

Electroanalysis

View full text Add to dashboard Cite

A glassy carbon electrode (GCE) modified with carbon nanotube/chitosan (MWCNTs-CHT/GCE) was used for the sensitive voltammetric determination of levodopa (Lev) and serotonin (Ser). The measurements were carried out using cyclic voltammetry (CV), differential pulse voltammetry (DPV) and chronoamperometry (CA). Under the optimum conditions the electrode provides a linear response versus Lev and Ser concentrations in the range of 2.0-220.0 mM and 0.5-130.0 mM, respectively, using DPV. The modified electrode was satisfactorily used for determination of Lev and Ser in human serum and urine with satisfactory results.

show abstract

“…It is also possible that any markers found in lavage fluid could ultimately be translated into stool markers in a final test. [68] Other Studies Levels of serotonin [67] histamine, tryptase, calprotectin, and eosinophilic cationic protein (ECP) [68] have been measured in WGLF samples in studies of subjective food hypersensitivity but none of these turned out to be a reliable marker, although ECP and histamine may warrant further study.…”

Section: Cancermentioning

confidence: 99%

Rectal Effluent as a Research Tool

2014

View full text Add to dashboard Cite

Studies of localized secretions are generally superior to those of blood because they contain higher concentrations of molecules specific to the organ of interest. A common method used to analyze localized secretions is lavage. The flow of fluid over the lining of a cavity picks up both cells and soluble factors, and the effluent can be collected for study. Gastrointestinal (GI) lavage is easily and noninvasively performed by the administration of gut lavage solutions such as those routinely given to patients prior to colonoscopy, with GI lavage fluid being the copious, watery rectal effluent subsequently induced. Residual effluent is currently suctioned from the colon and discarded during colonoscopy. With millions of routine colonoscopies performed per year, GI lavage fluid is a rich and largely untapped resource for basic and clinical research. Rectal effluent can also be easily collected in a toilet receptacle without need for a colonoscopy. Rectal effluent generated in this manner has been used to study diarrheal disease, mucosal immunology, inflammatory bowel disease, celiac disease, and cancer. It is often referred to as gut lavage, colon lavage, GI lavage, or whole gut lavage fluid, which makes it challenging to locate previous studies in the literature and there are currently no comprehensive reviews of its use as a research tool. This review attempts to fill this void by discussing previous applications of rectal effluent in research and the methods that have been developed for its collection, stabilization, and analysis.

show abstract

Direct determination of serotonin in gut lavage fluid by liquid chromatographic ion trap tandem mass spectrometry

Cited by 18 publications

References 157 publications

Quantitative profiling of tryptophan metabolites in serum, urine, and cell culture supernatants by liquid chromatography–tandem mass spectrometry

Quantitative profiling of tryptophan metabolites in serum, urine, and cell culture supernatants by liquid chromatography–tandem mass spectrometry

A Selective Simultaneous Determination of Levodopa and Serotonin Using a Glassy Carbon Electrode Modified with Multiwalled Carbon Nanotube/Chitosan Composite

Rectal Effluent as a Research Tool

Contact Info

Product

Resources

About